Academic Journal
Immunocastration with recombinant GnRH6-CRM197 fusion protein improves reproductive suppression and meat tenderness in roosters.
| Τίτλος: | Immunocastration with recombinant GnRH6-CRM197 fusion protein improves reproductive suppression and meat tenderness in roosters. |
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| Συγγραφείς: | Li M; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Han K; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Fu D; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Zhang R; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Jiang X; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Lu J; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Yan Y; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Li J; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China., Liu Y; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China., Li Y; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China., Yu T; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China., Duan H; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China., Fang F; Department of Basic Veterinary, College of Veterinary Medicine, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: fgfang@ahau.edu.cn. |
| Πηγή: | Poultry science [Poult Sci] 2026 Aug; Vol. 105 (8), pp. 107046. Date of Electronic Publication: 2026 May 01. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2020- : [Cambridge, UK] : Elsevier Original Publication: Champaign Il : Poultry Science Association |
| Ιατρικοί όροι (MeSH): | Chickens*/physiology , Chickens*/growth & development , Chickens*/immunology , Gonadotropin-Releasing Hormone*/immunology , Gonadotropin-Releasing Hormone*/administration & dosage , Meat*/analysis , Orchiectomy*/veterinary , Orchiectomy*/methods , Reproduction*/drug effects , Bacterial Proteins*/administration & dosage, Recombinant Fusion Proteins/immunology ; Testosterone/blood ; Testis/drug effects ; Animals ; Male |
| Περίληψη: | This study evaluated the potential of the recombinant GnRH6-CRM197 fusion protein as an immunocastration vaccine for roosters. Forty 4-week-old roosters were divided into two groups: the immune group and the control group that received a saline injection. The vaccine (1 mg/mL) was administered at 4 and 8 weeks of age. Body weights and blood samples were collected at 4, 8, 12, 16, and 20 weeks of age, with culling performed at 20 weeks. Serum anti-GnRH antibody titers and testosterone concentrations were measured. The testicles were collected for morphological analysis, while pectoral and thigh muscle samples were assessed for muscle fiber characteristics, intramuscular fat, and meat quality indicators. The results demonstrated that the immune group exhibited a significant induction of anti-GnRH antibodies (P < 0.05). Vaccination significantly suppressed comb and wattle development (persisting until week 16) and markedly reduced testis size, weight, and serum testosterone levels (P < 0.05). Histological examination confirmed a severe reduction in germ cell numbers at all developmental stages. Regarding the quality of the meat, the tenderness of the breast exhibited a significant enhancement in the group that had been immunized. However, no significant differences were observed among groups in live weight, carcass yield, muscle pH, meat color, or intramuscular fat content (P > 0.05). In summary, the GnRH6-CRM197 vaccine effectively suppresses reproductive function in roosters while enhancing meat tenderness, demonstrating potential for application as an immunocastration agent in poultry without adversely affecting key production indicators. (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Disclosures The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
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| Contributed Indexing: | Keywords: Recombinant GnRH6-CRM197; Reproductive function; Rooster |
| Substance Nomenclature: | 33515-09-2 (Gonadotropin-Releasing Hormone) 0 (Recombinant Fusion Proteins) 3XMK78S47O (Testosterone) 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20260507 Date Completed: 20260724 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13157140 |
| DOI: | 10.1016/j.psj.2026.107046 |
| PMID: | 42096907 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1525-3171 |
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| DOI: | 10.1016/j.psj.2026.107046 |