Engineering an Optogenetic pH-Modulator in Bacteria.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Engineering an Optogenetic pH-Modulator in Bacteria.
Συγγραφείς:	Kuang J; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Armendarez OJ; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Chang WT; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA., Hausladen MM; Kostas Research Institute, Northeastern University, Burlington, Massachusetts, USA., Bonanno S; Department of Bioengineering, Northeastern University, Boston, Massachusetts, USA., Wilson DJ; Kostas Research Institute, Northeastern University, Burlington, Massachusetts, USA.; Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, USA., Joshi NS; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA.; Department of Bioengineering, Northeastern University, Boston, Massachusetts, USA., Deravi LF; Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA.
Πηγή:	Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2026 Jul; Vol. 13 (42), pp. e24319. Date of Electronic Publication: 2026 May 07.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE
Imprint Name(s):	Original Publication: Weinheim : WILEY-VCH, [2014]-
Ιατρικοί όροι (MeSH):	Optogenetics/methods , Urease/genetics , Urease/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , Genetic Engineering/methods , Bacteria/genetics , Bacteria/metabolism, Synthetic Biology/methods ; Promoter Regions, Genetic/genetics ; Hydrogen-Ion Concentration
Περίληψη:	Cells in many naturally occurring organisms routinely cooperate to control their extracellular pH in a dynamic and reversible manner, but this capability has been underexplored in synthetic biology. Here, we sought to engineer a microbial system that switches between two states -high and low extracellular pH- with minimal human intervention. We accomplished this by combining: (1) a genetic circuit that produces recombinant urease under the control of a light-inducible promoter; (2) a degradation tag on urease to accelerate the high-to-low pH transition; and (3) optimization of several environmental factors, including media composition, replenishment rate, and light exposure patterns. The system raises the pH when urease is produced and hydrolyzes urea in the media to produce ammonia; it lowers the pH as a byproduct of the cell's native metabolism when urease production ceases. We demonstrate that the optimized system cycles continuously for up to 14 days with minimal performance loss. Overall, our system demonstrates synthetic pH control in an engineered living system and highlights challenges and potential solutions for using such systems outside of the context of typical laboratory manipulation. (© 2026 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
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Grant Information:	Government of the United States
Contributed Indexing:	Keywords: deployable living systems; engineered living material; light‐responsive bacteria; optogenetics; pH modulation
Substance Nomenclature:	EC 3.5.1.5 (Urease)
Entry Date(s):	Date Created: 20260507 Date Completed: 20260729 Latest Revision: 20260729
Update Code:	20260730
PubMed Central ID:	PMC13335442
DOI:	10.1002/advs.202524319
PMID:	42095488
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	2198-3844
DOI:	10.1002/advs.202524319