Academic Journal
Nitric Oxide-releasing Nanofibers Prevent Restenosis After Arterial Injury in a Renal Failure Model.
| Τίτλος: | Nitric Oxide-releasing Nanofibers Prevent Restenosis After Arterial Injury in a Renal Failure Model. |
|---|---|
| Συγγραφείς: | Binion CC; Department of Surgery, University of Virginia, Charlottesville, Virginia; Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, Virginia; University of North Carolina Chapel Hill School of Medicine, Chapel Hill, North Carolina., Tannhauser PA; Department of Surgery, University of Virginia, Charlottesville, Virginia., Barlek MH; Department of Surgery, University of Virginia, Charlottesville, Virginia., Gillis DC; Department of Surgery, University of Virginia, Charlottesville, Virginia., Yu L; Department of Surgery, University of Virginia, Charlottesville, Virginia., Karver MR; Northwestern University Center for Regenerative Medicine, Chicago, Illinois; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois., Stupp SI; Northwestern University Center for Regenerative Medicine, Chicago, Illinois; Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois; Department of Chemistry, Northwestern University, Evanston, Illinois; Department of Materials Science & Engineering, Northwestern University, Evanston, Illinois; Department of Biomedical Engineering, Northwestern University, Technological Institute, Evanston, Illinois., Tsihlis ND; Department of Surgery, University of Virginia, Charlottesville, Virginia; Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, Virginia., Kibbe MR; Department of Surgery, University of Virginia, Charlottesville, Virginia; Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, Virginia; Department of Cardiothoracic and Vascular Surgery, University of Texas Health Science Center at Houston, Houston, Texas. Electronic address: melina.kibbe@uth.tmc.edu. |
| Πηγή: | The Journal of surgical research [J Surg Res] 2026 Sep; Vol. 325, pp. 746-756. Date of Electronic Publication: 2026 May 05. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Academic Press Country of Publication: United States NLM ID: 0376340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8673 (Electronic) Linking ISSN: 00224804 NLM ISO Abbreviation: J Surg Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York, NY : Academic Press Original Publication: Philadelphia [etc.] |
| Ιατρικοί όροι (MeSH): | Carotid Artery Injuries*/complications , Carotid Artery Injuries*/pathology , Carotid Artery Injuries*/drug therapy , Neointima*/prevention & control , Neointima*/pathology , Neointima*/etiology , Nitric Oxide*/administration & dosage , Renal Insufficiency*/complications , Nanofibers*, Hyperplasia/prevention & control ; Animals ; Male ; Rats, Sprague-Dawley ; Rats ; Disease Models, Animal |
| Περίληψη: | Background: Restenosis from neointimal hyperplasia is a common cause of failure for vascular interventions. Targeted nitric oxide (NO)-releasing peptide amphiphile (PA) nanofibers have been successfully developed to prevent neointimal hyperplasia after vascular injury; however, it remains unknown if comorbid renal failure will affect the efficacy of this nanotherapeutic. We hypothesize that NO-releasing targeted nanofibers will effectively inhibit neointimal hyperplasia following arterial injury in a rat model of renal failure. Methods: PA molecules were synthesized, purified, and co-assembled into nanofibers, which were visualized by transmission electron microscopy (TEM). Twelve-week-old male Sprague Dawley rats (n= 6-7/group) underwent a 5/6th nephrectomy, followed by carotid artery balloon injury. Rats received saline, targeted nanofiber, or NO-releasing targeted nanofiber. Hemodynamics and blood chemistries were assessed at baseline, 2 wk, and 4 wk, followed by morphometric analysis of carotid arteries. Results: TEM confirmed nanofiber formation for all co-assemblies. Renal failure was confirmed with elevated creatinine (0.70 versus 0.35 mg/dL, P< 0.05), blood urea nitrogen (27.3 versus 18.1 mg/dL, P< 0.05), and mean arterial pressure (116.3 versus 94.8 mmHg, P< 0.05) 2 wk post-nephrectomy compared to baseline. Rats with renal failure which underwent balloon arterial injury and were treated with the NO-releasing targeted nanofiber developed 57.6% less neointimal hyperplasia compared to saline controls (59,136 versus 139,356 μm2, P< 0.001). Conclusions: This study demonstrates a single injection of an NO-releasing targeted nanofiber inhibits neointimal hyperplasia in the setting of renal failure with efficacy comparable to historic data in healthy rats. These data have positive implications for translation of this NO-releasing nanotherapeutic to clinical care. (Copyright © 2026 Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: Nanofiber; Neointimal hyperplasia; Nitric oxide; Renal failure; Restenosis |
| Substance Nomenclature: | 31C4KY9ESH (Nitric Oxide) |
| Entry Date(s): | Date Created: 20260506 Date Completed: 20260729 Latest Revision: 20260729 |
| Update Code: | 20260730 |
| DOI: | 10.1016/j.jss.2026.03.115 |
| PMID: | 42091348 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1095-8673 |
|---|---|
| DOI: | 10.1016/j.jss.2026.03.115 |