Academic Journal
Click chemistry reveals sialylated glycoprotein biosynthesis in Ixodes ricinus ticks and tick-derived cells.
| Τίτλος: | Click chemistry reveals sialylated glycoprotein biosynthesis in Ixodes ricinus ticks and tick-derived cells. |
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| Συγγραφείς: | Sterbova J; University of South Bohemia, Faculty of Science, Department of Chemistry, Ceske Budejovice, Czech Republic., Ondrus J; University of South Bohemia, Faculty of Science, Department of Chemistry, Ceske Budejovice, Czech Republic., Fussy Z; University of South Bohemia, Faculty of Science, Department of Chemistry, Ceske Budejovice, Czech Republic; Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, USA., Vechtova P; University of South Bohemia, Faculty of Science, Department of Chemistry, Ceske Budejovice, Czech Republic., Grubhoffer L; University of South Bohemia, Faculty of Science, Department of Chemistry, Ceske Budejovice, Czech Republic; Biology Centre of the Czech Academy of Sciences, Institute of Parasitology, Ceske Budejovice, Czech Republic., Sterba J; University of South Bohemia, Faculty of Science, Department of Chemistry, Ceske Budejovice, Czech Republic. Electronic address: sterbaj@prf.jcu.cz. |
| Πηγή: | International journal of biological macromolecules [Int J Biol Macromol] 2026 Jun; Vol. 365, pp. 152305. Date of Electronic Publication: 2026 Apr 30. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| Ιατρικοί όροι (MeSH): | Ixodes*/metabolism , Ixodes*/genetics , Glycoproteins*/biosynthesis , Glycoproteins*/chemistry , N-Acetylneuraminic Acid*/metabolism , N-Acetylneuraminic Acid*/chemistry , Click Chemistry*, Sialyltransferases/metabolism ; Sialyltransferases/genetics ; Animals ; Female ; Cell Line |
| Περίληψη: | Sialic acid commonly decorates the surface of cells and secreted proteins of Eukaryotes. It plays roles in cell signalling and adhesion, affects immune reactions and alternative complement pathways, and neurogenesis. While sialic acid is a saccharide present in high abundance in vertebrates, its minor representation was reported in arthropods and among medically necessary parasites. Ixodes ricinus tick is a significant vector of pathogens causing severe infections in humans and livestock. It has been known mainly through indirect evidence that sialylated glycoproteins are present in tick tissues. As a blood-feeding parasite, the host's blood, full of sialylated molecules, could be the source of such glycoproteins within the tick's body. To detect solely the tick sialylated glycoproteins, we used an azide-modified precursor of sialic acid to be metabolically incorporated by the tick cell line IRE/CTVM20 originating in this tick or by ticks if the corresponding biosynthetic pathway is present and active. Our I. ricinus genome screen revealed two sialyltransferase genes, which were expressed in all ticks' life stages, and we confirmed sialyltransferase activity in tick cells. We combined in vitro feeding along with Click chemistry to track the presence of sialylated glycoproteins from fed female ticks through their eggs to the larvae. Similarly, sialylated glycoproteins were produced by the IRE/CTVM20 cell line. Thus, we confirmed the ability of ticks to produce their own sialylated glycoproteins in addition to those originating from the host blood. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Click chemistry; Sialic acid; Tick |
| Substance Nomenclature: | 0 (Glycoproteins) GZP2782OP0 (N-Acetylneuraminic Acid) EC 2.4.99.- (Sialyltransferases) |
| Entry Date(s): | Date Created: 20260501 Date Completed: 20260716 Latest Revision: 20260716 |
| Update Code: | 20260716 |
| DOI: | 10.1016/j.ijbiomac.2026.152305 |
| PMID: | 42067082 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2026.152305 |