Academic Journal
A validated chiral LC-MS/MS method for enantioselective quantification of aromatic lactic acids in human faeces.
| Τίτλος: | A validated chiral LC-MS/MS method for enantioselective quantification of aromatic lactic acids in human faeces. |
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| Συγγραφείς: | Gaafar AM; Department of Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg C, Denmark., Stolberg-Mathieu G; Department of Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg C, Denmark., Roager HM; Department of Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg C, Denmark. Electronic address: hero@nexs.ku.dk., La Barbera G; Department of Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg C, Denmark. Electronic address: glb@nexs.ku.dk. |
| Πηγή: | Journal of chromatography. A [J Chromatogr A] 2026 Jul 19; Vol. 1779, pp. 466997. Date of Electronic Publication: 2026 Apr 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3778 (Electronic) Linking ISSN: 00219673 NLM ISO Abbreviation: J Chromatogr A Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, 1993- |
| Ιατρικοί όροι (MeSH): | Feces*/chemistry , Liquid Chromatography-Mass Spectrometry*/methods , Tandem Mass Spectrometry*/methods , Lactates*/analysis , Lactates*/chemistry, Lactic Acid/analysis ; Lactic Acid/chemistry ; Humans ; Stereoisomerism ; Reproducibility of Results ; Limit of Detection |
| Περίληψη: | Bifidobacterium, a key genus of the infant gut microbiome, produces d- and l- enantiomers of aromatic lactic acids that may influence early-life immune development through stereochemistry-dependent biological activity. No validated analytical methods currently enable their accurate enantio‑separation and quantification in human biological samples. We report the first validated, targeted, liquid chromatography-mass spectrometry method using a chiral column for the enantioselective separation and quantification of d- and l- forms of phenyllactic acid (PLA), 4-hydroxyphenyllactic acid (4OH-PLA), and indolelactic acid (ILA) in faecal samples. The method achieves baseline separation of all enantiomers within 10 min, with resolution values of 2.66 (PLA), 1.77 (4OH-PLA), and 2.42 (ILA). Solid-phase extraction reduces matrix effects (>80 %) and improves analyte recovery (>80 %). Limits of quantification range from 2.9 to 6.7 ng mL-1, and calibration curves show excellent linearity (R² > 0.99). Inter- and intra-day precision expressed as %RSD are < 15 % for most analytes. The method was successfully applied to infant faecal samples, enabling sensitive and stereospecific quantification of aromatic lactic acids, thus establishing a foundation for exploring the biological relevance of these enantiomers in infant health. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: 4-hydroxyphenyllactic acid; Aromatic lactic acids; Chiral chromatography; Gut microbiome; Indolelactic acid; Infants; LC-MS/MS; Method validation; Phenyllactic acid; Targeted metabolomics |
| Substance Nomenclature: | 0 (Lactates) 33X04XA5AT (Lactic Acid) |
| Entry Date(s): | Date Created: 20260428 Date Completed: 20260716 Latest Revision: 20260716 |
| Update Code: | 20260716 |
| DOI: | 10.1016/j.chroma.2026.466997 |
| PMID: | 42048934 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-3778 |
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| DOI: | 10.1016/j.chroma.2026.466997 |