Academic Journal
Reproducible Quantification of Pseudomonas aeruginosa via MIP-Functionalized SPR-FPI Optical Fiber Sensor.
| Τίτλος: | Reproducible Quantification of Pseudomonas aeruginosa via MIP-Functionalized SPR-FPI Optical Fiber Sensor. |
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| Συγγραφείς: | Zheng W, Kong W, Zhang N, Li Z, Zhang YN |
| Πηγή: | IEEE transactions on nanobioscience [IEEE Trans Nanobioscience] 2026 Oct; Vol. 25 (4), pp. 435-442. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Institute of Electrical and Electronics Engineers Country of Publication: United States NLM ID: 101152869 Publication Model: Print Cited Medium: Internet ISSN: 1558-2639 (Electronic) Linking ISSN: 15361241 NLM ISO Abbreviation: IEEE Trans Nanobioscience Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Piscataway, NJ : Institute of Electrical and Electronics Engineers, c2002- |
| Ιατρικοί όροι (MeSH): | Pseudomonas aeruginosa*/isolation & purification , Surface Plasmon Resonance*/instrumentation , Surface Plasmon Resonance*/methods , Molecular Imprinting*/methods , Interferometry*/instrumentation , Interferometry*/methods , Biosensing Techniques*/instrumentation , Fiber Optic Technology*/instrumentation, Polymers/chemistry ; Indoles/chemistry ; Optical Fibers ; Reproducibility of Results ; Equipment Design |
| Περίληψη: | A reusable optical fiber surface plasmon resonance (SPR) sensor for quantitative detection of Pseudomonas aeruginosa is presented. To achieve repeatable measurement, this study used aptamer DNA as the functional monomer and polydopamine as the cross-linking agent to construct a molecularly imprinted nanofilm on the sensing surface. After elution treatment, molecularly imprinted cavities with high matching shape and size to the target molecule are formed in the nanofilm. Experimental results demonstrated that the sensors enhanced by molecular imprinting technology achieved an increase in resonance wavelength response from 10.677 nm to 24.98 nm, a reduction in response time from 22 minutes to 16 minutes, and an improvement in detection limit from 0.00985 OD to 0.00105 OD, compared with sensors only modified with functional monomers. Moreover, to address the problem of temperature interference in practical applications, a Fabry-Perot interferometer (FPI) is innovatively integrated. By taking advantage of the temperature sensitivity but bacterial insensitivity of FPI, the influence of temperature changes on SPR measurement is quantified, effectively providing temperature compensation and further improving the environmental adaptability of the sensor. This study provides a novel, reliable, and cost-effective detection solution for marine environmental microbial monitoring. |
| Substance Nomenclature: | 0 (Polymers) 0 (Indoles) 0 (polydopamine) |
| Entry Date(s): | Date Created: 20260428 Date Completed: 20261002 Latest Revision: 20261005 |
| Update Code: | 20261005 |
| DOI: | 10.1109/TNB.2026.3687229 |
| PMID: | 42048224 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1558-2639 |
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| DOI: | 10.1109/TNB.2026.3687229 |