Academic Journal
Toxic effects of 5-hydroxymethylfurfural on intestinal epithelial cell and its impact on the expression of MAPK pathway-related genes.
| Τίτλος: | Toxic effects of 5-hydroxymethylfurfural on intestinal epithelial cell and its impact on the expression of MAPK pathway-related genes. |
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| Συγγραφείς: | Qin D; Scientific Research Base of Tea Comprehensive Utilization Technology Integration of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Chen X; Scientific Research Base of Tea Comprehensive Utilization Technology Integration of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Pan K; Scientific Research Base of Tea Comprehensive Utilization Technology Integration of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Tang Z; Scientific Research Base of Tea Comprehensive Utilization Technology Integration of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Chen Z; Scientific Research Base of Tea Comprehensive Utilization Technology Integration of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Lin X; Scientific Research Base of Tea Comprehensive Utilization Technology Integration of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China., Zhang Y; Scientific Research Base of Tea Comprehensive Utilization Technology Integration of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: zhangyy@scau.edu.cn. |
| Πηγή: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2026 Aug; Vol. 214, pp. 116112. Date of Electronic Publication: 2026 Apr 24. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8207483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6351 (Electronic) Linking ISSN: 02786915 NLM ISO Abbreviation: Food Chem Toxicol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Exeter : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, c1982- |
| Ιατρικοί όροι (MeSH): | Furaldehyde*/analogs & derivatives , Furaldehyde*/toxicity , Epithelial Cells*/drug effects , Epithelial Cells*/metabolism , Intestinal Mucosa*/drug effects , Intestinal Mucosa*/cytology , Intestinal Mucosa*/metabolism , MAP Kinase Signaling System*/drug effects , MAP Kinase Signaling System*/genetics , Intestines*/cytology , Intestines*/drug effects, Apoptosis/drug effects ; Reactive Oxygen Species/metabolism ; Oxidative Stress/drug effects ; Cell Proliferation/drug effects ; Gene Expression Regulation/drug effects ; NF-kappa B/metabolism ; NF-kappa B/genetics ; Interleukin-6/metabolism ; Interleukin-6/genetics ; Humans ; Cell Line |
| Περίληψη: | 5-Hydroxymethylfurfural (5-HMF), a common by-product of thermal-processed foods, poses a potential risk of intestinal toxicity upon ingestion. However, its underlying toxic mechanisms remain incompletely understood. In this study, we investigated the effects and potential molecular pathways of 5-HMF using normal human intestinal epithelial cells (HIEC-6). The results showed that 5-HMF inhibited the proliferation of HIEC-6 cells in a concentration-dependent manner and induced apoptosis possibly through both the death receptor and mitochondrial pathways, as evidenced by increased activities of caspase-8 and caspase-9.5-HMF increased intracellular reactive oxygen species (ROS) levels by 1.42 to 2.12 fold, disrupted antioxidant enzymes activities, and increased malondialdehyde content, indicating oxidative damage. Meanwhile, 5-HMF upregulated the mRNA expression of the pro-inflammatory cytokines TNF-α and IL-6 and downregulated the mRNA and protein expression of key tight junction proteins ZO-1, Occludin and Claudin-1, indicating promotion of inflammation and impairment of epithelial barrier. 5-HMF also altered the mRNA expression of MAPK pathway-associated genes and NF-κB, indicating a possible correlation between this signaling cascade and the toxic effects of 5-HMF. Collectively, these findings provide a theoretical foundation for the risk assessment of dietary 5-HMF exposure and for future mechanistic studies on the role of MAPK signaling in 5-HMF-induced intestinal toxicity. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: 5-Hydroxymethylfurfural; Apoptosis; HIEC-6 cells; Inflammation; Intestinal toxicity; Oxidative stress; Tight junction |
| Substance Nomenclature: | DJ1HGI319P (Furaldehyde) 70ETD81LF0 (5-hydroxymethylfurfural) 0 (Reactive Oxygen Species) 0 (NF-kappa B) 0 (Interleukin-6) |
| Entry Date(s): | Date Created: 20260426 Date Completed: 20260610 Latest Revision: 20260610 |
| Update Code: | 20260610 |
| DOI: | 10.1016/j.fct.2026.116112 |
| PMID: | 42035979 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-6351 |
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| DOI: | 10.1016/j.fct.2026.116112 |