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Xuebijing alleviates septic myocardial injury by inhibiting ferroptosis mediated by the ICAM1/TLR signaling pathway.

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Τίτλος: Xuebijing alleviates septic myocardial injury by inhibiting ferroptosis mediated by the ICAM1/TLR signaling pathway.
Συγγραφείς: Meng X; Critical Care Medicine, Xuzhou Mining Group General Hospital, Xuzhou, PR China., Yan X; Emergency Department, Xuzhou Mining Group General Hospital, Xuzhou, PR China. yxm13593070445@163.com., Kan C; Occupational Disease Department, Xuzhou Mining Group General Hospital, Xuzhou, PR China., Li H; Critical Care Medicine, Xuzhou Mining Group General Hospital, Xuzhou, PR China., Han D; Critical Care Medicine, Xuzhou Mining Group General Hospital, Xuzhou, PR China., Guo Y; Critical Care Medicine, Xuzhou Mining Group General Hospital, Xuzhou, PR China., Xue P; Critical Care Medicine, Xuzhou Mining Group General Hospital, Xuzhou, PR China., Jiang Y; Critical Care Medicine, Xuzhou Mining Group General Hospital, Xuzhou, PR China.
Πηγή: Histology and histopathology [Histol Histopathol] 2026 Nov; Vol. 41 (11), pp. 1865-1876. Date of Electronic Publication: 2026 Apr 23.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Histology and Histopathology Country of Publication: Spain NLM ID: 8609357 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1699-5848 (Electronic) Linking ISSN: 02133911 NLM ISO Abbreviation: Histol Histopathol Subsets: MEDLINE
Imprint Name(s): Publication: Murcia : Histology and Histopathology
Original Publication: Murcia, Spain : Gutenberg, 1986-
Ιατρικοί όροι (MeSH): Signal Transduction*/drug effects , Sepsis*/metabolism , Sepsis*/drug therapy , Sepsis*/complications , Sepsis*/pathology , Intercellular Adhesion Molecule-1*/metabolism , Drugs, Chinese Herbal*/pharmacology , Toll-Like Receptors*/metabolism , Ferroptosis*/drug effects, Myocytes, Cardiac/drug effects ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/pathology ; Animals ; Mice ; Male ; Disease Models, Animal ; Mice, Inbred C57BL
Περίληψη: Background: Xuebijing (XBJ) has demonstrated efficacy in alleviating clinical symptoms in sepsis patients; however, its underlying mechanism requires further investigation.
Methods: In this study, we established mouse and cardiomyocyte sepsis models using LPS. Cardiac pathological changes and ICAM1 expression were assessed through HE staining and immunohistochemistry. The expression of ICAM1, TFR1, ASCL4, TLR1, TBK1, and IFR7 was analyzed via PCR and western blotting. Cardiomyocyte survival and apoptosis were evaluated using the MTT assay and flow cytometry, respectively. Additionally, cTnI, BNP, CK-MB, IL-1β, IL-6, and TNF-α levels, along with GSH and MDA levels, were measured.
Results: HE staining confirmed the successful construction of the LPS-induced mouse model. XBJ inhibited ICAM1 expression in myocardial tissue as well as cells. Overexpression of ICAM1 suppressed survival and GSH levels while promoting apoptosis along with increased levels of cTnI, BNP, CK-MB, IL-1β, IL-6, TNF-α, and MDA of myocardial cells. Furthermore, ICAM1 activated Toll-like receptor (TLR) signaling pathways. Conversely, ICAM shRNA exhibited opposite effects. XBJ inhibited the overactivated TLR signaling pathway, inflammatory responses, and apoptosis induced by ICAM overexpression.
Conclusion: XBJ ameliorates myocardial injury in sepsis by inhibiting the ICAM-regulated TLR signaling pathway.
(©The Author(s) 2026. Open Access. This article is licensed under a Creative Commons CC-BY International License.)
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Substance Nomenclature: 126547-89-5 (Intercellular Adhesion Molecule-1)
0 (Drugs, Chinese Herbal)
0 (Xuebijing)
0 (Toll-Like Receptors)
0 (Icam1 protein, mouse)
Entry Date(s): Date Created: 20260423 Date Completed: 20261008 Latest Revision: 20261008
Update Code: 20261009
DOI: 10.14670/HH-25-079
PMID: 42023484
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1699-5848
DOI:10.14670/HH-25-079