Academic Journal
A functional deep cervical lymphovenous anastomosis model in APP/PS1 mice augmenting cerebral lymphatic drainage.
| Τίτλος: | A functional deep cervical lymphovenous anastomosis model in APP/PS1 mice augmenting cerebral lymphatic drainage. |
|---|---|
| Συγγραφείς: | Liu Y; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Jiang Y; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Zhao Z; Department of Neurosurgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China., Zhang H; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Li D; Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China., Chai G; Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China., Zhao P; Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China., Xu B; Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China., Jin Z; Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China., Qiu F; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Chen D; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Zhu E; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Shao W; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Zhu A; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Xu R; Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China., Gui S; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Liu Y; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China., Chen J; Department of Neurosurgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China., Hu R; Department of Neurosurgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China. Electronic address: huchrong@tmmu.edu.cn., Lu H; Department of Neurosurgery, Affiliated Hospital of Jiangnan University, Wuxi, China. Electronic address: 1414898701@qq.com. |
| Πηγή: | Journal of neuroscience methods [J Neurosci Methods] 2026 Aug; Vol. 432, pp. 110779. Date of Electronic Publication: 2026 Apr 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7905558 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-678X (Electronic) Linking ISSN: 01650270 NLM ISO Abbreviation: J Neurosci Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| Ιατρικοί όροι (MeSH): | Alzheimer Disease*/physiopathology , Anastomosis, Surgical*/methods , Lymphatic Vessels*/surgery , Lymph Nodes*/surgery , Jugular Veins*/surgery , Disease Models, Animal*, Amyloid beta-Protein Precursor/genetics ; Presenilin-1/genetics ; Animals ; Male ; Mice, Transgenic ; Indocyanine Green ; Cisterna Magna ; Mice |
| Περίληψη: | Background: Deep cervical lymphatics constitute the final pathway for cerebral lymphatic drainage into the circulatory system. Dysfunction in this pathway inevitably impairs the clearance of cerebral metabolic waste. Recent clinical study has reported the efficacy of deep cervical lymphovenous anastomosis (dcLVA) in Alzheimer's disease (AD) patients. However, the lack of a mouse animal model for LVA has hindered mechanistic research. New Method: To address this gap, we established a dcLVA model in APP/PS1 mice. Ten-month-old male APP/PS1 transgenic mice and wild-type (WT) littermates underwent side-to-side anastomosis between deep cervical lymph node and external jugular vein using supermicrosurgical techniques. Indocyanine green (ICG) was injected into the cisterna magna for evaluating cerebrospinal lymphatic drainage via near-infrared fluorescence tracking. Fluorescence intensity of deep cervical lymph node was dynamically recorded at 10 min, 30 min, 1 h, 2 h, 3 h, and 6 h post-ICG injection. Results: Results revealed significantly impaired ICG drainage in AD mice compared to WT littermates. Notably, dcLVA enhanced ICG drainage from subarachnoid space of cisterna magna in AD mice. Comparison With Existing Methods: This study is the first to establish a functional LVA model in AD mouse, demonstrating its ability to augment cerebral lymphatic drainage. Conclusions: Our findings provide a novel platform for future study to explore the effect and underlying mechanisms of LVA on AD and other neurodegenerative disorders associated with impaired lymphatic function. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest All authors have seen and approved the final version of the manuscript being submitted. They warrant that the article is the authors' original work, hasn't received prior publication and isn't under consideration for publication elsewhere. The authors declare that they have no conflict of interest. |
| Contributed Indexing: | Keywords: Alzheimer's Disease; Cerebral Lymphatic Drainage; Deep Cervical Lymphatics; Lymphovenous Anastomosis; Supermicrosurgery |
| Substance Nomenclature: | 0 (Amyloid beta-Protein Precursor) 0 (Presenilin-1) IX6J1063HV (Indocyanine Green) |
| Entry Date(s): | Date Created: 20260420 Date Completed: 20260715 Latest Revision: 20260715 |
| Update Code: | 20260715 |
| DOI: | 10.1016/j.jneumeth.2026.110779 |
| PMID: | 42009141 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1872-678X |
|---|---|
| DOI: | 10.1016/j.jneumeth.2026.110779 |