Academic Journal
Engineering Bacillus subtilis as a sustainable platform for the production of functional bile acids.
| Τίτλος: | Engineering Bacillus subtilis as a sustainable platform for the production of functional bile acids. |
|---|---|
| Συγγραφείς: | Zang Y; School of Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; JITRI Future Food Technology Research Institute Co., Ltd, Yixing, Jiangsu 214200, China., Guo Z; School of Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; JITRI Future Food Technology Research Institute Co., Ltd, Yixing, Jiangsu 214200, China; Wuxi Food Safety Inspection and Test Center, Technology Innovation Center of Special Food for State Market Regulation, Wuxi, Jiangsu 214122, China. Electronic address: zpguo@jiangnan.edu.cn., Ledesma-Amaro R; Bezos Centre for Sustainable Protein, London SW7 2AZ, UK; Microbial Food Hub, Imperial College Centre for Engineering Biology, Imperial College London, London SW7 2AZ, UK; Department of Bioengineering, Imperial College London, London SW7 2AZ, UK., Xin Y; School of Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; JITRI Future Food Technology Research Institute Co., Ltd, Yixing, Jiangsu 214200, China., Wu Y; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China., Gu Z; School of Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; JITRI Future Food Technology Research Institute Co., Ltd, Yixing, Jiangsu 214200, China., Zhang L; School of Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; JITRI Future Food Technology Research Institute Co., Ltd, Yixing, Jiangsu 214200, China; Engineering Research Center of the Ministry of Education for Wound Repair Technology, Wuxi, China. Electronic address: zhangl@jiangnan.edu.cn. |
| Πηγή: | Trends in biotechnology [Trends Biotechnol] 2026 Sep; Vol. 44 (9), pp. 2679-2698. Date of Electronic Publication: 2026 Apr 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Publishers Country of Publication: England NLM ID: 8310903 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3096 (Electronic) Linking ISSN: 01677799 NLM ISO Abbreviation: Trends Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Barking : Elsevier Science Publishers Original Publication: [Amsterdam, Netherlands : Elsevier Science Publishers B.V. (Biomedical Division), c1983- |
| Ιατρικοί όροι (MeSH): | Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , Bile Acids and Salts*/biosynthesis , Bile Acids and Salts*/metabolism , Metabolic Engineering*/methods |
| Περίληψη: | Bile acids and their derivatives are key regulators of host metabolism, immunity, and microbiome interactions, with growing therapeutic potential. Conventional production methods of bile acids relying on animal extraction or chemical synthesis are inefficient, costly, and environmentally unfriendly. Microbial synthesis offers a sustainable alternative but is limited by the absence of genetic tools for native anaerobic producers. Here, we reconstructed the bile acid 7α-dehydroxylation pathway in Bacillus subtilis, a safe and tractable host. We characterized the key Bai enzymes and performed comprehensive analyses of the bile acids generated through in vitro assays and whole-cell catalysis. Multiple intermediates and end products, including cholyl-CoA, 3-oxo-cholic acid, 3-oxo-4,5-6,7-didehydro-deoxycholic acid, 3-oxo-4,5-dehydro-deoxycholic acid, 3-oxo-deoxycholic acid, and deoxycholic acid, were identified. This work establishes a functional heterologous platform for bile acid biosynthesis, enabling sustainable production and future applications in therapeutics and microbiome engineering. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of interests The authors declare no competing interests. |
| Contributed Indexing: | Keywords: Bacillus subtilis; bile acid 7α-dehydroxylation pathway; engineering biology; metabolic engineering; synthetic biology |
| Substance Nomenclature: | 0 (Bile Acids and Salts) |
| Entry Date(s): | Date Created: 20260416 Date Completed: 20260902 Latest Revision: 20260915 |
| Update Code: | 20260915 |
| DOI: | 10.1016/j.tibtech.2026.03.021 |
| PMID: | 41991462 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-3096 |
|---|---|
| DOI: | 10.1016/j.tibtech.2026.03.021 |