Academic Journal
Rapid and Dense Immobilization of Nucleic Acids on Lanthanide-Doped Nanoparticles for Enhanced-Sensitivity Förster Resonance Energy Transfer-Based Diagnostics.
| Τίτλος: | Rapid and Dense Immobilization of Nucleic Acids on Lanthanide-Doped Nanoparticles for Enhanced-Sensitivity Förster Resonance Energy Transfer-Based Diagnostics. |
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| Συγγραφείς: | Song M; Department of Applied Physics, The Hong Kong Polytechnic University, Kowloon, Hong Kong 999077, P. R. China.; Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, People's Republic of China., Li L; Future Institute of Technology, MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510630, People's Republic of China., Zhang B; Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, People's Republic of China., Wong MC; Department of Applied Physics, The Hong Kong Polytechnic University, Kowloon, Hong Kong 999077, P. R. China., Lao X; Department of Applied Physics, The Hong Kong Polytechnic University, Kowloon, Hong Kong 999077, P. R. China., Liu J; Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada., Wei B; Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, People's Republic of China., Hao J; Department of Applied Physics, The Hong Kong Polytechnic University, Kowloon, Hong Kong 999077, P. R. China.; Research Center for Nanoscience and Nanotechnology, The Hong Kong Polytechnic University, Kowloon, Hong Kong 999077, People's Republic of China. |
| Πηγή: | ACS sensors [ACS Sens] 2026 Apr 24; Vol. 11 (4), pp. 3309-3321. Date of Electronic Publication: 2026 Apr 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Chemical Society, [2016]- |
| Ιατρικοί όροι (MeSH): | Fluorescence Resonance Energy Transfer*/methods , Lanthanoid Series Elements*/chemistry , Biosensing Techniques*/methods , SARS-CoV-2*/isolation & purification , SARS-CoV-2*/genetics , COVID-19*/diagnosis , COVID-19*/virology , Immobilized Nucleic Acids*/chemistry , Nanoparticles*/chemistry, Metal Nanoparticles/chemistry ; Humans ; Limit of Detection |
| Περίληψη: | Rapid and dense DNA functionalization of upconversion nanoparticles (UCNPs) remains a critical bottleneck, as conventional covalent and electrostatic methods suffer from low labeling efficiency and time-consuming processing. We report a microwave-assisted rapid dehydration method for efficient DNA immobilization on UCNPs within 3 min, drastically outperforming conventional strategies. This rapid dehydration method demonstrates broad applicability across diverse UCNP compositions, morphologies, and different DNA chain lengths. Molecular dynamics simulations confirm the underlying mechanism: strong noncovalent interactions between DNA phosphate groups/bases and lanthanide ions. Utilizing this approach, we constructed a FRET biosensor by integrating the DNA-functionalized UCNPs with Nb |
| Contributed Indexing: | Keywords: FRET; MXene; fast dehydrating; nucleic acid; ultrasensitive detection; upconverison nanoparticles |
| Substance Nomenclature: | 0 (Lanthanoid Series Elements) 0 (Immobilized Nucleic Acids) |
| Entry Date(s): | Date Created: 20260415 Date Completed: 20260715 Latest Revision: 20260715 |
| Update Code: | 20260715 |
| DOI: | 10.1021/acssensors.5c04782 |
| PMID: | 41983568 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2379-3694 |
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| DOI: | 10.1021/acssensors.5c04782 |