Academic Journal
Προβολή σε EDS

A Live-Cell Fluorescence High-Throughput Screening Method for Antimicrobial Peptides Based on the SpyTag/SpyCatcher System.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: A Live-Cell Fluorescence High-Throughput Screening Method for Antimicrobial Peptides Based on the SpyTag/SpyCatcher System.
Συγγραφείς: Li J; Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China.; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China., Wen S; Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China.; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China., Wang F; Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China.; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China.
Πηγή: Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Apr 22; Vol. 74 (15), pp. 12261-12270. Date of Electronic Publication: 2026 Apr 14.
Τύπος έκδοσης: Journal Article; Evaluation Study
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
Imprint Name(s): Original Publication: Washington, American Chemical Society.
Ιατρικοί όροι (MeSH): High-Throughput Screening Assays*/methods , High-Throughput Screening Assays*/instrumentation , Antimicrobial Peptides*/pharmacology , Antimicrobial Peptides*/chemistry , Fluorescence Resonance Energy Transfer*/methods , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry , Antimicrobial Cationic Peptides*/pharmacology , Antimicrobial Cationic Peptides*/chemistry, Escherichia coli/drug effects ; Escherichia coli/growth & development ; Bacillus subtilis/drug effects ; Microbial Sensitivity Tests ; Fluorescence
Περίληψη: Antimicrobial peptides (AMPs) are key to innate immunity but face challenges in rational design due to their diversity and complex mechanisms. The lack of sensitive and full function-based high-throughput screening methods for AMP variants has further impeded systematic exploration and optimization. To address this, we developed a high-throughput screening method for AMPs integrating SpyTag/SpyCatcher click biology and fluorescence resonance energy transfer (FRET). Attributing to the ultrahigh reaction rate and specificity of the SpyTag/SpyCatcher pair, this screening method allows real-time fluorometric detection of the cell lytic activity of AMPs against their natural targets, i.e., bacterial cells. Applied to an indolicidin proline-scanning library, this approach identified variants with enhanced activity against E. coli and B. subtilis. Collectively, this work establishes a function-based, high-throughput screening platform for AMPs that can fully reflect their functional complexity, thereby providing an efficient and scalable tool for screening AMP libraries and facilitating data-driven optimization and functional analysis.
Contributed Indexing: Keywords: FRET; SpyTag/SpyCatcher; antimicrobial peptides; high-throughput screening
Substance Nomenclature: 0 (Antimicrobial Peptides)
0 (Anti-Bacterial Agents)
0 (Antimicrobial Cationic Peptides)
Entry Date(s): Date Created: 20260414 Date Completed: 20260715 Latest Revision: 20260715
Update Code: 20260715
DOI: 10.1021/acs.jafc.5c17550
PMID: 41978950
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 41978950
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: A Live-Cell Fluorescence High-Throughput Screening Method for Antimicrobial Peptides Based on the SpyTag/SpyCatcher System.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Li+J%22">Li J</searchLink>; Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China.; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China.<br /><searchLink fieldCode="AU" term="%22Wen+S%22">Wen S</searchLink>; Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China.; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China.<br /><searchLink fieldCode="AU" term="%22Wang+F%22">Wang F</searchLink>; Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing 100048, China.; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%220374755%22">Journal of agricultural and food chemistry</searchLink> [J Agric Food Chem] 2026 Apr 22; Vol. 74 (15), pp. 12261-12270. <i>Date of Electronic Publication: </i>2026 Apr 14.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article; Evaluation Study
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22American+Chemical+Society%22">American Chemical Society </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>0374755 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1520-5118 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200218561%22">00218561 </searchLink><i>NLM ISO Abbreviation: </i>J Agric Food Chem <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: Washington, American Chemical Society.
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22High-Throughput+Screening+Assays%22">High-Throughput Screening Assays*</searchLink>/<searchLink fieldCode="MM" term="%22High-Throughput+Screening+Assays+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22High-Throughput+Screening+Assays%22">High-Throughput Screening Assays*</searchLink>/<searchLink fieldCode="MM" term="%22High-Throughput+Screening+Assays+instrumentation%22">instrumentation</searchLink> <br /><searchLink fieldCode="MM" term="%22Antimicrobial+Peptides%22">Antimicrobial Peptides*</searchLink>/<searchLink fieldCode="MM" term="%22Antimicrobial+Peptides+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Antimicrobial+Peptides%22">Antimicrobial Peptides*</searchLink>/<searchLink fieldCode="MM" term="%22Antimicrobial+Peptides+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Fluorescence+Resonance+Energy+Transfer%22">Fluorescence Resonance Energy Transfer*</searchLink>/<searchLink fieldCode="MM" term="%22Fluorescence+Resonance+Energy+Transfer+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Anti-Bacterial+Agents%22">Anti-Bacterial Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Anti-Bacterial+Agents+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Anti-Bacterial+Agents%22">Anti-Bacterial Agents*</searchLink>/<searchLink fieldCode="MM" term="%22Anti-Bacterial+Agents+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Antimicrobial+Cationic+Peptides%22">Antimicrobial Cationic Peptides*</searchLink>/<searchLink fieldCode="MM" term="%22Antimicrobial+Cationic+Peptides+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Antimicrobial+Cationic+Peptides%22">Antimicrobial Cationic Peptides*</searchLink>/<searchLink fieldCode="MM" term="%22Antimicrobial+Cationic+Peptides+chemistry%22">chemistry</searchLink><br /><searchLink fieldCode="MH" term="%22Escherichia+coli%22">Escherichia coli</searchLink>/<searchLink fieldCode="MH" term="%22Escherichia+coli+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Escherichia+coli%22">Escherichia coli</searchLink>/<searchLink fieldCode="MH" term="%22Escherichia+coli+growth+%26+development%22">growth & development</searchLink> ; <searchLink fieldCode="MH" term="%22Bacillus+subtilis%22">Bacillus subtilis</searchLink>/<searchLink fieldCode="MH" term="%22Bacillus+subtilis+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Microbial+Sensitivity+Tests%22">Microbial Sensitivity Tests</searchLink> ; <searchLink fieldCode="MH" term="%22Fluorescence%22">Fluorescence</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Antimicrobial peptides (AMPs) are key to innate immunity but face challenges in rational design due to their diversity and complex mechanisms. The lack of sensitive and full function-based high-throughput screening methods for AMP variants has further impeded systematic exploration and optimization. To address this, we developed a high-throughput screening method for AMPs integrating SpyTag/SpyCatcher click biology and fluorescence resonance energy transfer (FRET). Attributing to the ultrahigh reaction rate and specificity of the SpyTag/SpyCatcher pair, this screening method allows real-time fluorometric detection of the cell lytic activity of AMPs against their natural targets, i.e., bacterial cells. Applied to an indolicidin proline-scanning library, this approach identified variants with enhanced activity against E. coli and B. subtilis. Collectively, this work establishes a function-based, high-throughput screening platform for AMPs that can fully reflect their functional complexity, thereby providing an efficient and scalable tool for screening AMP libraries and facilitating data-driven optimization and functional analysis.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>FRET; SpyTag/SpyCatcher; antimicrobial peptides; high-throughput screening
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Antimicrobial Peptides)<br />0 (Anti-Bacterial Agents)<br />0 (Antimicrobial Cationic Peptides)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260414 <i>Date Completed: </i>20260715 <i>Latest Revision: </i>20260715
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260715
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1021/acs.jafc.5c17550
– Name: AN
  Label: PMID
  Group: ID
  Data: 41978950
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=41978950
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1021/acs.jafc.5c17550
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 12261
    Subjects:
      – SubjectFull: Escherichia coli drug effects
        Type: general
      – SubjectFull: Escherichia coli growth & development
        Type: general
      – SubjectFull: Bacillus subtilis drug effects
        Type: general
      – SubjectFull: Microbial Sensitivity Tests
        Type: general
      – SubjectFull: Fluorescence
        Type: general
      – SubjectFull: High-Throughput Screening Assays methods
        Type: general
      – SubjectFull: High-Throughput Screening Assays instrumentation
        Type: general
      – SubjectFull: Antimicrobial Peptides pharmacology
        Type: general
      – SubjectFull: Antimicrobial Peptides chemistry
        Type: general
      – SubjectFull: Fluorescence Resonance Energy Transfer methods
        Type: general
      – SubjectFull: Anti-Bacterial Agents pharmacology
        Type: general
      – SubjectFull: Anti-Bacterial Agents chemistry
        Type: general
      – SubjectFull: Antimicrobial Cationic Peptides pharmacology
        Type: general
      – SubjectFull: Antimicrobial Cationic Peptides chemistry
        Type: general
    Titles:
      – TitleFull: A Live-Cell Fluorescence High-Throughput Screening Method for Antimicrobial Peptides Based on the SpyTag/SpyCatcher System.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Li J
      – PersonEntity:
          Name:
            NameFull: Wen S
      – PersonEntity:
          Name:
            NameFull: Wang F
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 22
              M: 04
              Text: 2026 Apr 22
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1520-5118
          Numbering:
            – Type: volume
              Value: 74
            – Type: issue
              Value: 15
          Titles:
            – TitleFull: Journal of agricultural and food chemistry
              Type: main
ResultId 1