Academic Journal
Therapeutic and toxicity targets for Euphorbia Kansui L. in treating hepatocellular carcinoma ascites revealed by ingredients pattern analysis accompanying vinegar processing.
| Τίτλος: | Therapeutic and toxicity targets for Euphorbia Kansui L. in treating hepatocellular carcinoma ascites revealed by ingredients pattern analysis accompanying vinegar processing. |
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| Συγγραφείς: | Ma M; Synergy Innovation Center of Biological Peptide Antidiabetics of Hubei Province, College of Life Science, Wuchang University of Technology, Wuhan 430223, China., Wu F; College of Traditional Chinese Medicine, Department of Medicine, Jianghan University, Wuhan 430056, China; Institute of Acupuncture and Moxibustion, Jianghan University, Wuhan 430056, China. Electronic address: Fanwu@jhun.edu.cn., Feng Y; Synergy Innovation Center of Biological Peptide Antidiabetics of Hubei Province, College of Life Science, Wuchang University of Technology, Wuhan 430223, China., Liu H; Synergy Innovation Center of Biological Peptide Antidiabetics of Hubei Province, College of Life Science, Wuchang University of Technology, Wuhan 430223, China., Jiang H; Pharmaceutical Research Institute, School of Chemical and Pharmaceutical Engineering, Wuhan Institute of Technology, Wuhan 430205, China. Electronic address: hapyia2100@sina.com. |
| Πηγή: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2026 Jun 01; Vol. 1277, pp. 125053. Date of Electronic Publication: 2026 Apr 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101139554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-376X (Electronic) Linking ISSN: 15700232 NLM ISO Abbreviation: J Chromatogr B Analyt Technol Biomed Life Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| Ιατρικοί όροι (MeSH): | Euphorbia*/chemistry , Ascites*/drug therapy , Ascites*/metabolism , Carcinoma, Hepatocellular*/drug therapy , Carcinoma, Hepatocellular*/metabolism , Liver Neoplasms*/drug therapy , Liver Neoplasms*/metabolism , Plant Extracts*/pharmacology , Plant Extracts*/chemistry , Plant Extracts*/toxicity , Drugs, Chinese Herbal*/chemistry , Drugs, Chinese Herbal*/pharmacology , Drugs, Chinese Herbal*/toxicity, Acetic Acid/chemistry ; Cell Proliferation/drug effects ; Humans ; Chromatography, High Pressure Liquid ; Cell Line, Tumor ; Molecular Docking Simulation |
| Περίληψη: | Euphorbia Kansui L. is effective in managing ascites resulting from hepatocirrhosis and hepatoma, however, it has some toxicity. Vinegar processing, a traditional detoxification method, can effectively reduce the toxicity of raw Kansui. However, the underlying mechanisms of Kansui's therapeutic and toxicological effects remain unclear. This topic was investigated through an integrated approach in current study. Firstly, the extract of Kansui was proved to have an inhibitory effect on the proliferation of various tumor cells, particularly liver cancer cells. Then HPLC-based ingredients pattern analysis on raw and processed Kansui indicated that there was a significant reduction for triterpenoids and jatrophane-type diterpenoids during the processing, in contrast, a mild reduction for ingenane-type diterpenoids was observed during the same processing. From network pharmacology analysis inferred that terpenoids from Kansui are involved in tumor signaling pathways and immune activation pathways and so on, may contribute to therapeutic effect of alleviate malignant ascites. Inflammatory response pathway and cellular response to chemical stimulus may contribute to the herb's toxicity such as gastrointestinal irritation. The core targets in the protein-protein interaction network were then validated by molecular docking, SPR experiments and enzymatic experiments. Enzyme inhibition assays confirming that extract of vinegar-processed Kansui exhibits reduced inhibitory effects on cholinesterases (BCHE and ACHE), aligning with predicted detoxification mechanisms. Besides, SPR analysis revealing weaker binding affinity of processed Kansui to the toxicological target ACHE, supporting the detoxification mechanism at the molecular level. These findings are meaningful for understanding the mechanism for Kansui's therapeutic and toxicological effects, meanwhile to ensure the safety of Kansui in medication. (Copyright © 2024. Published by Elsevier B.V.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Enzyme inhibition experiments; Euphorbia Kansui L.; Network pharmacology; SPR analysis; Target prediction |
| Substance Nomenclature: | Q40Q9N063P (Acetic Acid) 0 (Plant Extracts) 0 (Drugs, Chinese Herbal) |
| Entry Date(s): | Date Created: 20260409 Date Completed: 20260714 Latest Revision: 20260714 |
| Update Code: | 20260715 |
| DOI: | 10.1016/j.jchromb.2026.125053 |
| PMID: | 41955719 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-376X |
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| DOI: | 10.1016/j.jchromb.2026.125053 |