Academic Journal
Poly(3-hydroxybutyrate) production from swine wastewater and waste vegetable oil by Cupriavidus necator in nonsterile bioreactors.
| Τίτλος: | Poly(3-hydroxybutyrate) production from swine wastewater and waste vegetable oil by Cupriavidus necator in nonsterile bioreactors. |
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| Συγγραφείς: | Cordero-Jorquera B; Biosystems Engineering Laboratory, Department of Chemical and Bioprocess Engineering, Universidad de Santiago de Chile (USACH), Santiago, Chile., Gutierrez-Urrutia I; Biosystems Engineering Laboratory, Department of Chemical and Bioprocess Engineering, Universidad de Santiago de Chile (USACH), Santiago, Chile., Guerra-Arredondo S; Biosystems Engineering Laboratory, Department of Chemical and Bioprocess Engineering, Universidad de Santiago de Chile (USACH), Santiago, Chile., Orellana-Saez M; Biosystems Engineering Laboratory, Department of Chemical and Bioprocess Engineering, Universidad de Santiago de Chile (USACH), Santiago, Chile., Fuentes I; Biosystems Engineering Laboratory, Department of Chemical and Bioprocess Engineering, Universidad de Santiago de Chile (USACH), Santiago, Chile., Jofre-Dupre P; Biosystems Engineering Laboratory, Department of Chemical and Bioprocess Engineering, Universidad de Santiago de Chile (USACH), Santiago, Chile; Departamento de Ingeniería Química y Ambiental, Universidad Técnica Federico Santa María, Santiago, Chile., Lebrero R; Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina s/n., Valladolid 47011, Spain; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n., Valladolid 47011, Spain., Rivero-Buceta V; Biotechnology Department, Margarita Salas Center for Biological Research (CIB Margarita Salas-CSIC), Madrid, Spain., Carvajal A; Departamento de Ingeniería Química y Ambiental, Universidad Técnica Federico Santa María, Santiago, Chile., Poblete-Castro I; Biosystems Engineering Laboratory, Department of Chemical and Bioprocess Engineering, Universidad de Santiago de Chile (USACH), Santiago, Chile. Electronic address: ignacio.poblete.c@usach.cl. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Jul; Vol. 451, pp. 134554. Date of Electronic Publication: 2026 Apr 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Bioreactors*/microbiology , Cupriavidus necator*/metabolism , Wastewater*/microbiology , Wastewater*/chemistry , Hydroxybutyrates*/metabolism , Polyesters*/metabolism , Plant Oils*/metabolism, Animals ; Polyhydroxybutyrates ; Swine |
| Περίληψη: | Swine wastewater (SW), rich in nitrogen and phosphorus, and waste vegetable oil (WVO) are abundant feedstocks with potential for microbial biopolymers production. However, high polyhydroxyalkanoates (PHA) yields under nonsterile conditions remain challenging due to inhibitory compounds and microbial competition. Here, we cultivated Cupriavidus necator DSM545 under nonsterile conditions with varying SW dilutions combined with WVO for PHA synthesis. Batch bioreactors containing two-thirds (v/v) non-centrifuged wastewater yielded the highest polyhydroxybutyrate titers of 6.8 ± 0.1 gPHB/L, with C. necator comprising over 80% of the microbial community. A nonsterile fed-batch reactor further enhanced PHB titers by 10-fold, reaching a volumetric productivity of 1.37 gPHB/L⋅h, where Cupriavidus dominated the community (>98%). The produced PHB exhibited a melting temperature of 170 °C, molecular weight of 437 KDa, and a polydispersity index of 2.1. These findings demonstrate that C. necator can outcompete native microorganisms in wastewater and convert nutrient-rich waste streams into bioplastics. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Cupriavidusnecator; Fed-batch; Nonsterile bioreactors; Poly(3-hydroxybutyrate); Swine wastewater; Waste vegetable oil |
| Substance Nomenclature: | 0 (Wastewater) 0 (Hydroxybutyrates) 0 (Polyesters) 0 (Plant Oils) 0 (Polyhydroxybutyrates) 26063-00-3 (poly-beta-hydroxybutyrate) |
| Entry Date(s): | Date Created: 20260405 Date Completed: 20260714 Latest Revision: 20260714 |
| Update Code: | 20260715 |
| DOI: | 10.1016/j.biortech.2026.134554 |
| PMID: | 41935698 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2026.134554 |