Academic Journal
Thiosulfate-Driven redox buffering enables efficient nitrogen removal and norfloxacin degradation in mixed denitrifying systems.
| Τίτλος: | Thiosulfate-Driven redox buffering enables efficient nitrogen removal and norfloxacin degradation in mixed denitrifying systems. |
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| Συγγραφείς: | Qian J; Research & Development Institute in Shenzhen, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, China., Li X; Research & Development Institute in Shenzhen, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, China., Xu X; The 2nd Geological Brigade of Sichuan, Chengdu, China; Observation and·Research Station of Ecological Restoration of Ruoergai Wetland in the Upper Reaches of the Yellow River, MNR, China., Zhang D; The 2nd Geological Brigade of Sichuan, Chengdu, China; Observation and·Research Station of Ecological Restoration of Ruoergai Wetland in the Upper Reaches of the Yellow River, MNR, China., Xiang G; The 2nd Geological Brigade of Sichuan, Chengdu, China; Observation and·Research Station of Ecological Restoration of Ruoergai Wetland in the Upper Reaches of the Yellow River, MNR, China., Wang Z; Research & Development Institute in Shenzhen, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, China., Zhang Z; College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China; Wens Foodstuff Group Co., Ltd, Yunfu 527400, China. Electronic address: zzhangal@scau.edu.cn., Liu M; Wens Foodstuff Group Co., Ltd, Yunfu 527400, China., Hao W; Research & Development Institute in Shenzhen, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, China. Electronic address: haowen@nwpu.edu.cn., Wu D; Department of Green Chemistry and Technology, Ghent University, Belgium. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Jul; Vol. 451, pp. 134550. Date of Electronic Publication: 2026 Apr 01. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Norfloxacin*/metabolism , Norfloxacin*/isolation & purification , Denitrification*/drug effects , Thiosulfates*/pharmacology , Thiosulfates*/chemistry , Nitrogen*/isolation & purification, Biodegradation, Environmental/drug effects ; Bioreactors/microbiology ; RNA, Ribosomal, 16S/genetics ; Oxidation-Reduction |
| Περίληψη: | Wastewaters often contain both conventional pollutants and recalcitrant antibiotics, posing challenges to biological treatment. This study investigated a mixed autotrophic-heterotrophic denitrification system driven by sodium acetate and sodium thiosulfate for simultaneous nitrate and norfloxacin removal. A sequencing batch reactor was operated in four stages, culminating in norfloxacin exposure (0.5 mg/L). Results showed stable nitrogen removal (>95%) and norfloxacin degradation (>90%) under sustained antibiotic stress. Batch tests confirmed that the co-presence of thiosulfate and acetate enhanced norfloxacin biodegradation via co-metabolic pathways, with negligible abiotic removal. Three-dimensional excitation-emission matrix spectroscopy revealed a shift toward humic-like extracellular polymeric substances under norfloxacin, supporting biofilm integrity. 16S rRNA sequencing and metagenomics indicated dynamic microbial restructuring, with persistent core taxa (Thauera, Desulfofustis) and enrichment of stress-tolerant groups (norank_o_SJA-15). Functional analysis showed upregulation of carbon metabolism (pta, ackA), denitrification (nirS, nosZ), and sulfur oxidation (SUOX, SoxX, SoxA) genes, alongside oxidative stress mitigation genes (catB, gst) and xenobiotic degradation genes (HGD, E1.13.11.4). Antibiotic resistance gene profiles shifted toward multidrug (>29%), peptide resistance (14.0%→15.4%), and glycopeptide resistance (7.0%→9.4%), dominated by multidrug efflux and target alteration mechanisms, enabling community resilience while minimizing energetically costly defenses. This work elucidates the synergistic roles of dual electron donors in pollutant co-removal and stress mitigation, offering a robust, sustainable strategy for treating antibiotic-laden wastewater. (Copyright © 2026. Published by Elsevier Ltd.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Antibiotic resistance genes; Extracellular polymeric substances; Metagenomic analysis; Mixed denitrification; Norfloxacin removal |
| Substance Nomenclature: | N0F8P22L1P (Norfloxacin) 0 (Thiosulfates) N762921K75 (Nitrogen) 0 (RNA, Ribosomal, 16S) |
| Entry Date(s): | Date Created: 20260403 Date Completed: 20260714 Latest Revision: 20260714 |
| Update Code: | 20260714 |
| DOI: | 10.1016/j.biortech.2026.134550 |
| PMID: | 41932524 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2026.134550 |