Academic Journal
Protocol for gene knockdown in Trichoplax adhaerens using a silica nanoparticle-mediated RNA interference technique.
| Τίτλος: | Protocol for gene knockdown in Trichoplax adhaerens using a silica nanoparticle-mediated RNA interference technique. |
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| Συγγραφείς: | Guo Q; Fang Zongxi Center for Marine EvoDevo, MoE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China., Li W; Fang Zongxi Center for Marine EvoDevo, MoE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: wanqing_li@ouc.edu.cn., Zheng W; Key Laboratory of Marine Chemistry Theory and Technology, Ocean University of China, Ministry of Education, Qingdao 266100, China., Qiu M; Key Laboratory of Marine Chemistry Theory and Technology, Ocean University of China, Ministry of Education, Qingdao 266100, China., Zhao C; Fang Zongxi Center for Marine EvoDevo, MoE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: chengtian_zhao@ouc.edu.cn. |
| Πηγή: | STAR protocols [STAR Protoc] 2026 Jun 19; Vol. 7 (2), pp. 104484. Date of Electronic Publication: 2026 Apr 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| Ιατρικοί όροι (MeSH): | Silicon Dioxide*/chemistry , Nanoparticles*/chemistry , Gene Knockdown Techniques*/methods , RNA Interference* |
| Περίληψη: | Here, we present a protocol for gene knockdown in Trichoplax adhaerens using a silica nanoparticle-mediated RNA interference (RNAi) technique. We describe steps for preparing silica nanoparticles, assembling RNAi complexes, and delivering them to live specimens. This protocol enables reproducible suppression of target gene expression in T. adhaerens and achieves transcript knockdown efficiencies of approximately 50%-70%, as assessed by quantitative PCR. For complete details on the use and execution of this protocol, please refer to Li et al.1. (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of interests The authors declare no competing interests. |
| References: | Science. 2018 Sep 28;361(6409):1377-1380. (PMID: 30262503) Nat Genet. 2012 Mar 04;44(4):381-9, S1-2. (PMID: 22387996) Nature. 2008 Aug 21;454(7207):955-60. (PMID: 18719581) Front Neurosci. 2023 Apr 13;17:1125624. (PMID: 37123368) Physiol Rep. 2022 Mar;10(5):e15206. (PMID: 35274831) Curr Biol. 2025 Sep 22;35(18):4577-4586.e4. (PMID: 40876458) Research (Wash D C). 2022 Dec 21;2022:0014. (PMID: 39290973) |
| Contributed Indexing: | Keywords: Evolutionary biology; Gene Expression; Molecular Biology |
| Substance Nomenclature: | 7631-86-9 (Silicon Dioxide) |
| Entry Date(s): | Date Created: 20260403 Date Completed: 20260623 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13084400 |
| DOI: | 10.1016/j.xpro.2026.104484 |
| PMID: | 41931393 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2026.104484 |