Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Polarized Phase-Sensitive Fluorescence-Image Correlation Spectroscopy. |
| Συγγραφείς: |
Clayton AHA; Optical Sciences Centre, Department of Physics and Astronomy, School of Science, Computing and Emerging Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia. |
| Πηγή: |
Biomolecules [Biomolecules] 2026 Mar 13; Vol. 16 (3). Date of Electronic Publication: 2026 Mar 13. |
| Τύπος έκδοσης: |
Journal Article |
| Γλώσσα: |
English |
| Στοιχεία περιοδικού: |
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE |
| Imprint Name(s): |
Original Publication: Basel, Switzerland : MDPI, 2011- |
| Ιατρικοί όροι (MeSH): |
Fluorescence Polarization*/methods, Spectrometry, Fluorescence/methods ; Microscopy, Fluorescence/methods |
| Περίληψη: |
Molecular interactions underpin the functioning of the living cell. Molecules exist in distinct quaternary structural forms, associate with molecular partners in signaling cascades, form transient quinary interactions, localize in membrane domains, and cluster in membrane-less condensates. Measuring the concentration, size, and dynamics of these molecular assemblies remains an enduring biophysical challenge, particularly in cells, where heterogeneity is the rule rather than the exception. Orthogonal signals derived from fluorescence lifetime, fluorescence fluctuations, and fluorescence polarization provide valuable metrics for probing interactions and environments, concentration and size, and rotational dynamics, respectively. This paper combines fluorescence lifetime imaging microscopy with image correlation analysis and polarization to determine the concentrations, brightness, lifetime, and rotational correlation time of different fluorescent states. A two-population model is examined as a prototypical example of a heterogeneous system. The analysis is illustrated on a simple fluorescence model system, where cluster densities, relative brightnesses, lifetimes, and rotational correlation times are extracted. |
| Contributed Indexing: |
Keywords: fluorescence lifetime imaging microscopy; image correlation spectroscopy; polarization |
| Entry Date(s): |
Date Created: 20260328 Date Completed: 20260713 Latest Revision: 20260713 |
| Update Code: |
20260714 |
| PubMed Central ID: |
PMC13023811 |
| DOI: |
10.3390/biom16030433 |
| PMID: |
41897368 |
| Βάση Δεδομένων: |
MEDLINE |