Academic Journal
Protein A/G-based microfluidic platform for preparative microseparation of IgG from human serum.
| Τίτλος: | Protein A/G-based microfluidic platform for preparative microseparation of IgG from human serum. |
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| Συγγραφείς: | Porycka K; Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664, Warsaw, Poland., Drozd M; Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664, Warsaw, Poland; Department of Medical Diagnostics, Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822, Warsaw, Poland. Electronic address: marcin.drozd.ch@pw.edu.pl., Tokarska K; Department of Medical Diagnostics, Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822, Warsaw, Poland., Żukowski K; Department of Medical Diagnostics, Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822, Warsaw, Poland., Rastawicki W; Department of Bacteriology and Biocontamination, National Institute of Public Health NIH-National Research Institute, 00-791, Warsaw, Poland., Pietrzak M; Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664, Warsaw, Poland; Department of Medical Diagnostics, Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822, Warsaw, Poland., Malinowska E; Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664, Warsaw, Poland; Department of Medical Diagnostics, Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822, Warsaw, Poland. |
| Πηγή: | Talanta [Talanta] 2026 Aug 01; Vol. 305, pp. 129682. Date of Electronic Publication: 2026 Mar 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| Ιατρικοί όροι (MeSH): | Immunoglobulin G*/blood , Immunoglobulin G*/isolation & purification , Staphylococcal Protein A*/chemistry , COVID-19*/diagnosis , COVID-19*/blood , COVID-19*/immunology , SARS-CoV-2*/immunology , COVID-19 Serological Testing*/methods , COVID-19 Serological Testing*/instrumentation , Antibodies, Viral*/blood , Antibodies, Viral*/isolation & purification , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods , Lab-On-A-Chip Devices*, Humans ; Enzyme-Linked Immunosorbent Assay |
| Περίληψη: | IgG antibodies represent the principal analytical target in serological assays due to their high antigen specificity and persistence in blood following infection or vaccination. However, such assays often suffer from matrix-derived background and variability that obscures diagnostic cut-offs. A pre-analytical sample treatment pathway offers a potential solution to these challenges, but existing IgG purification tools are often incompatible with miniaturized detection systems. This gap motivates the development of miniaturized, automation-ready pre-analytical modules for antibody separation. We developed an open-channel PET Lab-on-a-Foil cassette in which protein A/G-functionalized microchannels are able to reversibly capture IgG. An automated flow-control unit runs a bind-wash-elute sequence with pH-triggered IgG release, yielding ∼30 μL of eluate per run. ELISA showed that IgG-enriched fractions had C-reactive protein, used as an internal standard, reduced to background levels, and only residual free protein A/G was detected, consistent with minimal matrix carryover and ligand leaching. SARS-CoV-2 serology was employed as a validation model due to its clinical relevance and well-characterized antibody responses. In the assay detecting IgG anti-SARS-CoV-2 nucleoprotein, positive-serum eluates gave a 7.6-fold higher antigen-specific signal than negative-serum eluates, while blank signals were lower than in matched raw sera. In SPR experiments, reference-channel responses for three sera decreased by 80-90% after pre-treatment, confirming reduced nonspecific adsorption. By integrating IgG capture and acidic elution in a disposable PET cassette that can be interfaced with ELISA and SPR, the platform lowers blank signal and its variance near diagnostic cut-offs and offers a practical route toward automation-compatible point-of-care immunodiagnostics. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Marcin Drozd and co-authors have patent #P.453652, "Microfluidic cassette for pre-analytical separation of immunoglobulin G (IgG) from serum samples, method of manufacturing the microfluidic cassette, and use of the microfluidic cassette for pre-analytical separation of immunoglobulin G (IgG) from serum samples" pending to Warsaw University of Technology. |
| Contributed Indexing: | Keywords: IgG separation; Lab-on-a-Foil; Microfluidic system; Protein A/G; Serological immunoassay; Surface plasmon resonance |
| Substance Nomenclature: | 0 (Immunoglobulin G) 0 (Staphylococcal Protein A) 0 (Antibodies, Viral) |
| Entry Date(s): | Date Created: 20260321 Date Completed: 20260711 Latest Revision: 20260711 |
| Update Code: | 20260711 |
| DOI: | 10.1016/j.talanta.2026.129682 |
| PMID: | 41863926 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2026.129682 |