PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens.
Συγγραφείς: Liu M; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China; Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China., Xi L; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China., Zhou Y; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China; Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China., Chen M; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China; Department of Bioengineering, College of Life Science, Henan Normal University, Xinxiang 453007, China., Qu H; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China., Ma B; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China., Zhang L; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China., Shi C; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China., Chen G; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China., Ren Z; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China., Cao J; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China; Shandong Haineng Bioengineering Co, Ltd, Rizhao 276800, China., Han J; Department of Animal Science, College of Smart Animal Husbandry, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China. Electronic address: j.c.han@hotmail.com.
Πηγή: Poultry science [Poult Sci] 2026 Jun; Vol. 105 (6), pp. 106762. Date of Electronic Publication: 2026 Mar 12.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE
Imprint Name(s): Publication: 2020- : [Cambridge, UK] : Elsevier
Original Publication: Champaign Il : Poultry Science Association
Ιατρικοί όροι (MeSH): Chickens*/metabolism , Chickens*/physiology , Chickens*/growth & development , Protein Kinase C*/metabolism , Protein Kinase C*/genetics , Cyclic AMP-Dependent Protein Kinases*/metabolism , Cyclic AMP-Dependent Protein Kinases*/genetics , Signal Transduction*/drug effects , Cholecalciferol*/metabolism , Cholecalciferol*/administration & dosage , Intestinal Absorption*/drug effects , Phosphorus, Dietary*/metabolism, Diet/veterinary ; Animal Feed/analysis ; Animals ; Male ; Random Allocation
Περίληψη: Three experiments were conducted to investigate the functional roles of the protein kinase A (PKA) and protein kinase C (PKC) signaling pathways in vitamin D₃ (VD₃)-regulated intestinal phosphorus (P) absorption in broiler chickens. Experiment 1 examined the effects of dietary VD3 levels on the PKA and PKC signaling pathways. Experiments 2 and 3 evaluated the effects of intraperitoneal injection of PKA and PKC inhibitors on intestinal P transporter expression. Results from Experiment 1 showed that 1000 IU/kg VD₃ improved growth performance (1-19 d), increased mRNA and protein expression of the jejunal NaPi-IIb phosphate transporter (P < 0.05), and affected the protein expression ratios of p-PKA/t-PKA, p-PI3K/t-PI3K, p-ERK/t-ERK, p-PKC/t-PKC and p-p38MAPK/t-p38MAPK (P < 0.05). In Experiment 2, the PKA inhibitor H-89 reduced mRNA and protein expression of jejunal NaPi-IIb in broilers (10-15 d) (P < 0.05). H-89 increased p-PI3K/t-PI3K and p-ERK/t-ERK protein expression ratios (P < 0.05), reduced the p-p38MAPK/t-p38MAPK ratio (P < 0.05), and had no effect on the p-PKC/t-PKC ratio (P > 0.05). Experiment 3 showed that the PKC inhibitor staurosporine decreased mRNA and protein expression of duodenal NaPi-IIb in broilers (9-13 d) (P < 0.05). Staurosporine increased the p-PKA/t-PKA protein expression ratio (P < 0.05), reduced the p-PI3K/t-PI3K and p-ERK/t-ERK ratios (P < 0.05), and had no effect on the p-p38MAPK/t-p38MAPK ratio (P > 0.05). Collectively, the PKA and PKC signaling pathways participate in VD₃-regulated intestinal P absorption in broiler chickens, and that the PKC pathway may function upstream of the PKA signaling pathway. This study lays a theoretical foundation for exploring the mineral homeostasis mechanisms by which VD₃ regulates intestinal P absorption in poultry.
(Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.)
Competing Interests: Disclosures The authors declare that they have no conflicts of interest.
Contributed Indexing: Keywords: NaPi-IIb; PKA signaling pathway; PKC signaling pathway; Vitamin D(3); broiler chicken
Substance Nomenclature: EC 2.7.11.13 (Protein Kinase C)
EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
1C6V77QF41 (Cholecalciferol)
0 (Phosphorus, Dietary)
Entry Date(s): Date Created: 20260319 Date Completed: 20260711 Latest Revision: 20260711
Update Code: 20260711
PubMed Central ID: PMC13011214
DOI: 10.1016/j.psj.2026.106762
PMID: 41855802
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1525-3171
DOI:10.1016/j.psj.2026.106762