Academic Journal
Bicarbonate buffer enhances functional sperm selection compared to Zwitterionic buffers in sperm preparation.
| Τίτλος: | Bicarbonate buffer enhances functional sperm selection compared to Zwitterionic buffers in sperm preparation. |
|---|---|
| Συγγραφείς: | Meitei HY; Centre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India.; Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India., Predheepan D; Centre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India.; Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India., Uppangala S; Division of Reproductive Genetics, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India., R VL; Department of Data Science, Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, 576104, India., Kalthur G; Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India., Schlatt S; Centre of Reproductive Medicine and Andrology (CeRA), University of Münster, Münster, Germany. stefan.schlatt@ukmuenster.de., Adiga SK; Centre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India. satish.adiga@manipal.edu.; Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India. satish.adiga@manipal.edu.; Centre for Fertility Preservation, Department of Reproductive Science, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, India. satish.adiga@manipal.edu. |
| Πηγή: | Scientific reports [Sci Rep] 2026 Mar 18; Vol. 16 (1). Date of Electronic Publication: 2026 Mar 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Ιατρικοί όροι (MeSH): | Spermatozoa*/drug effects , Spermatozoa*/cytology , Spermatozoa*/physiology , Bicarbonates*/pharmacology , Bicarbonates*/chemistry, Sperm Motility/drug effects ; HEPES/pharmacology ; Acrosome Reaction/drug effects ; Male ; Humans ; Buffers ; Morpholines |
| Περίληψη: | Sperm preparation media used in assisted reproductive technology (ART) laboratories typically contain single or combined buffers to maintain pH and support spermatozoa homeostasis. Concerns exist that zwitterionic buffers, such as 4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid (HEPES) and 3-(N-morpholino) propane sulfonic acid (MOPS), may negatively affect intrinsic cellular functions. Hence, this prospective study evaluated the impact of various buffers on sperm selection by swim up from fifty-four sub-fertile men, which were then divided into normozoospermic samples (n = 27) and non-normozoospermic (n = 27) cohorts. Each ejaculate was split into six aliquots, and swim-up was performed with media buffered by bicarbonate, HEPES, MOPS, bicarbonate + HEPES, or bicarbonate + MOPS. Post-swim-up spermatozoa were assessed for motility, morphology, kinematics, acrosome reaction, mitochondrial integrity, and DNA damage. Bicarbonate-buffered medium yielded spermatozoa with significantly higher motility and mitochondrial activity in both normozoospermic and non-normozoospermic samples (p < 0.001). In contrast, media containing HEPES or MOPS were associated with reduced motility (p < 0.001), compromised sperm kinematic parameters (p < 0.05), and diminished mitochondrial potential (p < 0.05). The study findings highlight the importance of bicarbonate buffer over zwitterionic buffers in sperm preparation media for the selection of better spermatozoa during ART procedures. |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: The study was approved by the Institutional Ethics Committee (IEC 520/2019) of Kasturba Medical College, Manipal Academy of Higher Education, Manipal. Consent for publication: All authors have given final approval for publication. |
| References: | Andrologia. 2021 Feb;53(1):e13666. (PMID: 32510691) Andrology. 2019 Jan;7(1):24-30. (PMID: 30225889) Andrology. 2014 Sep;2(5):678-86. (PMID: 24947544) Clin Exp Reprod Med. 2015 Mar;42(1):22-9. (PMID: 25874170) Science. 1971 Oct 29;174(4008):500-3. (PMID: 5110427) Reprod Biol Endocrinol. 2014 Dec 02;12:121. (PMID: 25466430) Andrology. 2025 Feb;13(2):382-395. (PMID: 38804843) Hum Reprod. 2019 Jan 01;34(1):3-11. (PMID: 30428044) Nat Commun. 2023 Sep 5;14(1):5395. (PMID: 37669933) Am J Physiol Heart Circ Physiol. 2005 Dec;289(6):H2503-7. (PMID: 16100247) Biomolecules. 2024 Jun 12;14(6):. (PMID: 38927088) J Androl. 2000 Jul-Aug;21(4):515-24. (PMID: 10901437) Andrology. 2024 May;12(4):881-890. (PMID: 37801310) J Cell Sci. 2001 Oct;114(Pt 19):3543-55. (PMID: 11682613) Nat Commun. 2021 Jun 22;12(1):3855. (PMID: 34158477) Methods Enzymol. 1972;24:53-68. (PMID: 4206745) Mol Hum Reprod. 2024 Feb 29;30(3):. (PMID: 38341666) Reprod Biomed Online. 2010 Jul;21(1):6-16. (PMID: 20570214) Mol Hum Reprod. 2005 Sep;11(9):683-91. (PMID: 16192296) Gamete Res. 1988 Feb;19(2):123-9. (PMID: 3209176) J Physiol. 2025 May;603(9):2633-2653. (PMID: 40320899) Fertil Steril. 2006 May;85(5):1415-9. (PMID: 16600227) Fertil Steril. 1991 Sep;56(3):540-6. (PMID: 1894034) Anal Biochem. 1980 May 15;104(2):300-10. (PMID: 7446957) J Assist Reprod Genet. 2011 Aug;28(8):711-24. (PMID: 21614519) Sci Rep. 2024 Jun 21;14(1):14287. (PMID: 38907001) Brain Res. 1996 Apr 22;717(1-2):69-75. (PMID: 8738255) Mol Hum Reprod. 2016 Feb;22(2):83-92. (PMID: 26621869) Reprod Sci. 2021 Jan;28(1):134-143. (PMID: 32734563) Reprod Biomed Online. 2009 Jun;18(6):799-810. (PMID: 19490784) Int J Mol Sci. 2022 Jun 06;23(11):. (PMID: 35683013) J Biol Chem. 2014 Mar 28;289(13):8735-41. (PMID: 24515117) Int J Androl. 2010 Feb;33(1):e180-6. (PMID: 19751363) Biophys J. 2011 Jul 20;101(2):362-9. (PMID: 21767488) Biol Reprod. 1986 Mar;34(2):349-56. (PMID: 3082381) Anal Biochem. 2006 Feb 15;349(2):262-7. (PMID: 16289439) Syst Biol Reprod Med. 2014 Aug;60(4):239-44. (PMID: 24673547) J Assist Reprod Genet. 2024 May;41(5):1341-1356. (PMID: 38436798) J Androl. 2003 Jan-Feb;24(1):59-66. (PMID: 12514084) Fertil Steril. 1997 Jan;67(1):98-103. (PMID: 8986691) |
| Contributed Indexing: | Keywords: Assisted reproductive techniques; Infertility; Sperm recovery; Sperm wash; Spermatozoa; Swim-up technique |
| Substance Nomenclature: | 0 (Buffers) 0 (Bicarbonates) RWW266YE9I (HEPES) 273BP63NV3 (morpholinopropane sulfonic acid) 0 (Morpholines) |
| Entry Date(s): | Date Created: 20260319 Date Completed: 20260627 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13003109 |
| DOI: | 10.1038/s41598-026-44733-9 |
| PMID: | 41851339 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2045-2322 |
|---|---|
| DOI: | 10.1038/s41598-026-44733-9 |