Academic Journal
Development of immunoliposomes with E-Selectin affinity to target endothelial inflammation: direct comparison of antibody types.
| Τίτλος: | Development of immunoliposomes with E-Selectin affinity to target endothelial inflammation: direct comparison of antibody types. |
|---|---|
| Συγγραφείς: | Gkartziou F; Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece., Petegnief V; Department of Neuroscience and Experimental Therapeutics, Biomedical Research Institute of Barcelona (IIBB-CSIC), Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi Sunyer, Barcelona, Spain., Kannavou M; Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece., Flavián-Lázaro AC; Department of Neuroscience and Experimental Therapeutics, Biomedical Research Institute of Barcelona (IIBB-CSIC), Barcelona, Spain., Pascual N; Nanobiotechnology for Diagnostics (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain., Marquez-Kisinousky L; Department of Neuroscience and Experimental Therapeutics, Biomedical Research Institute of Barcelona (IIBB-CSIC), Barcelona, Spain., Marco P; Nanobiotechnology for Diagnostics (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain., Planas AM; Department of Neuroscience and Experimental Therapeutics, Biomedical Research Institute of Barcelona (IIBB-CSIC), Barcelona, Spain.; Institut d'Investigacions Biomèdiques August Pi Sunyer, Barcelona, Spain., Antimisiaris SG; Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.; Institute of Chemical Engineering Sciences, FORTH/ICE-HT, Patras, Greece. |
| Πηγή: | Journal of liposome research [J Liposome Res] 2026 Sep; Vol. 36 (3), pp. 303-315. Date of Electronic Publication: 2026 Mar 18. |
| Τύπος έκδοσης: | Journal Article; Comparative Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 9001952 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2394 (Electronic) Linking ISSN: 08982104 NLM ISO Abbreviation: J Liposome Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: London : Informa Healthcare Original Publication: New York, N.Y. : Dekker, c1988- |
| Ιατρικοί όροι (MeSH): | E-Selectin*/immunology , E-Selectin*/metabolism , Liposomes*/chemistry , Liposomes*/immunology , Antibodies, Monoclonal*/immunology , Antibodies, Monoclonal*/chemistry , Inflammation*/drug therapy , Inflammation*/immunology , Endothelial Cells*/immunology , Endothelial Cells*/metabolism , Endothelial Cells*/drug effects, Polyethylene Glycols/chemistry ; Animals ; Humans |
| Περίληψη: | Inflamed endothelium targeting via E-Selectin is a promising strategy for drug delivery across the neurovascular interface, however, exploitation of the strategy by immunoliposomes remains limited. Furthermore, the high cost of antibodies poses a practical barrier for iterative formulation optimization. In this study, we developed two E-Selectin-targeted immunoliposome types incorporating either a commercially available monoclonal antibody (mAb) or a mAb produced in-house from a proprietary hybridoma line. Pre-formed liposomes were functionalized through maleimide-PEG conjugation and characterized for size distribution (by DLS), zeta potential (electrophoretic mobility), and Mab attachment (%). Immunoliposome targeting efficacy was assessed on brain microvascular endothelial cells under basal conditions and also after LPS-induced inflammation (to mimic pathological E-Selectin upregulation), by flow cytometry. Both immunoliposome formulations had similar characteristics and demonstrated significantly enhanced binding to endothelial cells relative to non-targeted liposomes, under both conditions studied. Furthermore, uptake by inflamed cells was always significantly higher than by non-inflamed cells. No differences in (in vitro) targeting magnitude between the commercial and hybridoma-derived Mab's were quantified, proving their comparable performance despite substantial differences in mAb cost. The current findings highlight the feasibility of substituting commercial mAbs with hybridoma-derived ones during preclinical development of targeted nanocarriers, as a strategy to reduce expenses while preserving their specificity and functional performance. Furthermore, it is demonstrated that E-Selectin targeted immunoliposomes are promising drug delivery systems for treatment of inflamed vasculature, justifying further in vitro and in vivo exploitation. |
| Contributed Indexing: | Keywords: E-Selectin; antibody; brain endothelia; cell uptake; hybridoma; immunoliposomes; inflammation |
| Substance Nomenclature: | 0 (E-Selectin) 0 (Liposomes) 0 (Antibodies, Monoclonal) 3WJQ0SDW1A (Polyethylene Glycols) |
| Entry Date(s): | Date Created: 20260318 Date Completed: 20260901 Latest Revision: 20260901 |
| Update Code: | 20260901 |
| DOI: | 10.1080/08982104.2026.2645864 |
| PMID: | 41848579 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1532-2394 |
|---|---|
| DOI: | 10.1080/08982104.2026.2645864 |