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Radioactive Springs and Archaeal Life in Deep Groundwater Systems.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Radioactive Springs and Archaeal Life in Deep Groundwater Systems.
Συγγραφείς: Eckertová T; Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynská Dolina F-1, Bratislava, 841 04, Slovak Republic., Palyzová A; Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, Prague, 142 00, Czech Republic., Műllerová M; Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynská Dolina F-1, Bratislava, 841 04, Slovak Republic., Řezanka T; Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, Prague, 142 00, Czech Republic. rezanka@biomed.cas.cz.
Πηγή: Microbial ecology [Microb Ecol] 2026 Mar 14; Vol. 89 (1). Date of Electronic Publication: 2026 Mar 14.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: United States NLM ID: 7500663 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-184X (Electronic) Linking ISSN: 00953628 NLM ISO Abbreviation: Microb Ecol Subsets: MEDLINE
Imprint Name(s): Original Publication: New York, Springer-Verlag.
Ιατρικοί όροι (MeSH): Archaea*/chemistry , Archaea*/isolation & purification , Archaea*/metabolism , Archaea*/classification , Groundwater*/microbiology , Groundwater*/chemistry , Natural Springs*/microbiology , Natural Springs*/chemistry, Lipids/analysis ; Glyceryl Ethers/analysis ; Lipidomics ; Temperature
Περίληψη: This study investigates the archaeal lipid distribution in freshwater springs with a particular focus on lipidomic profiles as ecological indicaters. Cultivation-independent approaches were employed to analyze organisms that had not yet been cultivated in the laboratory. Shotgun lipidomics of 21 springs in western and central part of Slovakia revealed more than 100 characteristic archaeal lipids, from which three biomarker groups were selected: (i) core lipids containing archaeol and glycerol dialkyl glycerol tetraethers (GDGT), including their mono and dihydroxy derivatives; (ii) mono- to tetra-glycosides of archaeol and GDGTs; and (iii) six phosphoarchaeols (archaeol-based phospholipids). Statistical analyses classified springs into three categories: cold (temperature < 20 °C), warm (> 30 °C), and radioactive (a subset of cold springs with ˃100 Bq/L radioactivity). Significant shifts in the ratios of archaeal lipids were correlated with the temperature and radioactivity, demonstrating the sensitivity of lipidomic profiling to environmental parameters. Moreover, tandem mass spectrometry identified a previously undescribed metabolite, archaeol-based dimethylphosphatidylethanolamine. The applied method provides rapid and highly sensitive tools for screening the presence of archaea, detecting as few as several thousand cells per liter, and offers new insights into the ecology of archaeal communities in groundwater environments.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
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Grant Information: (VEGA project No. 1/0019/22 Scientific Grant Agency; CZ.02.01.01/00/22_008/0004597 Grant Talking Microbes; APVV-21-0356 Slovak Research and Development Agency; RVO 61388971 Institutional Research Concept
Contributed Indexing: Keywords: Archaea; Groundwater ecology; Mass spectrometry; Microbial communities; Radioactive springs; Shotgun lipidomics
Substance Nomenclature: 0 (Lipids)
0 (Glyceryl Ethers)
0 (archaeol lipid)
Entry Date(s): Date Created: 20260314 Date Completed: 20260627 Latest Revision: 20260627
Update Code: 20260627
PubMed Central ID: PMC13032954
DOI: 10.1007/s00248-026-02720-7
PMID: 41826531
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1432-184X
DOI:10.1007/s00248-026-02720-7