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Dehydration-driven organization of metabolites into NaDES-like assemblies in orthodox seeds.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Dehydration-driven organization of metabolites into NaDES-like assemblies in orthodox seeds.
Συγγραφείς: Haddad Y; IGEPP UMR-1349, INRAE, University of Rennes, Institut Agro Rennes-Angers, Le Rheu, F-35650, France.; ISCR UMR-6226, University of Rennes, CNRS, ENSCR, Rennes, F-35042, France., Delhaye T; IETR UMR-6164, University of Rennes, Rennes, F-35042, France., Limanton E; ISCR UMR-6226, University of Rennes, CNRS, ENSCR, Rennes, F-35042, France., Morineau D; IPR UMR-6251, University of Rennes, CNRS, Rennes, F-35042, France., Le Corvec M; ScanMAT, University of Rennes, CNRS, ScanMAT-UAR2025, Rennes, F-35042, France., Deponge C; ScanMAT, University of Rennes, CNRS, ScanMAT-UAR2025, Rennes, F-35042, France., Levrel A; IGEPP UMR-1349, INRAE, University of Rennes, Institut Agro Rennes-Angers, Le Rheu, F-35650, France., Moréac A; IPR UMR-6251, University of Rennes, CNRS, Rennes, F-35042, France., Nazabal V; ISCR UMR-6226, University of Rennes, CNRS, ENSCR, Rennes, F-35042, France., Rondeau D; IETR UMR-6164, University of Rennes, Rennes, F-35042, France., Paquin L; ISCR UMR-6226, University of Rennes, CNRS, ENSCR, Rennes, F-35042, France., Bouchereau A; IGEPP UMR-1349, INRAE, University of Rennes, Institut Agro Rennes-Angers, Le Rheu, F-35650, France.; Metabolic Profiling and Metabolomic Platform (P2M2), METABOHUB, IGEPP, Le Rheu, F-35000, France.
Πηγή: The New phytologist [New Phytol] 2026 Aug; Vol. 251 (3), pp. 1172-1187. Date of Electronic Publication: 2026 Mar 12.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
Imprint Name(s): Publication: Oxford : Wiley on behalf of New Phytologist Trust
Original Publication: London, New York [etc.] Academic Press.
Ιατρικοί όροι (MeSH): Seeds*/metabolism , Seeds*/physiology , Brassica napus*/metabolism , Brassica napus*/physiology , Desiccation* , Metabolome*, Sucrose/metabolism ; Water/metabolism ; Amino Acids/metabolism ; Dehydration
Περίληψη: Desiccation tolerance in plants, especially during orthodox seed dehydration, relies on compatible solute accumulation, complex molecular mechanisms, and intermolecular organizations that remain poorly understood. We combined metabolite profiling, mass spectrometry imaging, and electrospray and cold-spray ionization mass spectrometry to investigate metabolite organization into natural deep eutectic solvent-like assemblies during oilseed rape seed dehydration. We show that sucrose colocalizes and interacts with organic and amino acids in seed tissues to form hydration-dependent, sucrose-centered assemblies, whose formation is promoted by water loss and reproduced in bioinspired artificial mixtures displaying NaDES-like physicochemical properties. These findings support the idea that seed metabolites, with sucrose as preferential matrix, organize into eutectic-like supramolecular networks during dehydration, suggesting a physicochemical basis for cytoplasmic stabilization in desiccation-tolerant seeds.
(© 2026 The Author(s). New Phytologist © 2026 New Phytologist Foundation.)
References: Angelovici R, Galili G, Fernie AR, Fait A. 2010. Seed desiccation: a bridge between maturation and germination. Trends in Plant Science 15: 211–218.
Berjak P, Pammenter N. 2013. Implications of the lack of desiccation tolerance in recalcitrant seeds. Frontiers in Plant Science 4: 478.
Bertrand E, Cousseau C, Kunapuli SVS, Delhaye T, Schurhammer R, Chaumont A, Limanton E, Legouin B, Paquin L, Castel X et al. 2025. Evaluating the strength of molecular interactions in a deep eutectic solvent (DES) by means of ionization mechanisms involved in cold‐spray ionization mass spectrometry and by DFT calculations. Physical Chemistry Chemical Physics (PCCP) 28: 1924–1938.
Bianchetti G, Baron C, Carrillo A, Berardocco S, Marnet N, Wagner M‐H, Demilly D, Ducournau S, Manzanares‐Dauleux MJ, Cahérec FL et al. 2021. Dataset for the metabolic and physiological characterization of seeds from oilseed rape (Brassica napus L.) plants grown under single or combined effects of drought and clubroot pathogen Plasmodiophora brassicae. Data in Brief 37: 107247.
Billi D, Potts M. 2002. Life and death of dried prokaryotes. Research in Microbiology 153: 7–12.
Bragagnolo FS, Strieder MM, Pizani RS, de Souza Mesquita LM, González‐Miquel M, Rostagno MA. 2024. Revisiting natural deep eutectic solvents (NADES) as extraction media and ready‐to‐use purposes. TrAC Trends in Analytical Chemistry 175: 117726.
Bubalo MC, Andreou T, Panić M, Radović M, Radošević K, Redovniković IR. 2023. Natural multi‐osmolyte cocktails form deep eutectic systems of unprecedented complexity: discovery, affordances and perspectives. Green Chemistry 25: 3398–3417.
Buitink J, Leprince O. 2004. Glass formation in plant anhydrobiotes: survival in the dry state. Cryobiology 48: 215–228.
Buitink J, Leprince O. 2008. Intracellular glasses and seed survival in the dry state. Comptes Rendus Biologies 331: 788–795.
Buzatu AR, Todea A, Peter F, Boeriu CG. 2024. The role of reactive natural deep eutectic solvents in sustainable biocatalysis. ChemCatChem 16: e202301597.
Carpenter JF, Crowe JH. 1988. Modes of stabilization of a protein by organic solutes during desiccation. Cryobiology 25: 459–470.
Choi YH, van Spronsen J, Dai Y, Verberne M, Hollmann F, Arends IWCE, Witkamp G‐J, Verpoorte R. 2011. Are natural deep eutectic solvents the missing link in understanding cellular metabolism and physiology? Plant Physiology 156: 1701–1705.
Choi YH, Verpoorte R. 2019. Green solvents for the extraction of bioactive compounds from natural products using ionic liquids and deep eutectic solvents. Current Opinion in Food Science 26: 87–93.
Dai Y, van Spronsen J, Witkamp G‐J, Verpoorte R, Choi YH. 2013. Natural deep eutectic solvents as new potential media for green technology. Analytica Chimica Acta 766: 61–68.
Dai Y, Varypataki EM, Golovina EA, Jiskoot W, Witkamp G‐J, Choi YH, Verpoorte R. 2021. Chapter Seven – Natural deep eutectic solvents in plants and plant cells: In vitro evidence for their possible functions. In: Verpoorte R, Witkamp G‐J, Choi YH, eds. Advances in Botanical Research. Eutectic solvents and stress in plants. Amsterdam, The Netherlands: Academic Press, 159–184.
Dai Y, Witkamp G‐J, Verpoorte R, Choi YH. 2015. Tailoring properties of natural deep eutectic solvents with water to facilitate their applications. Food Chemistry 187: 14–19.
Dekkers BJW, Costa MCD, Maia J, Bentsink L, Ligterink W, Hilhorst HWM. 2015. Acquisition and loss of desiccation tolerance in seeds: from experimental model to biological relevance. Planta 241: 563–577.
Durand E, Villeneuve P, Bourlieu‐lacanal C, Carrière F. 2021. Chapter six – Natural deep eutectic solvents: hypothesis for their possible roles in cellular functions and interaction with membranes and other organized biological systems. In: Verpoorte R, Witkamp G‐J, Choi YH, eds. Advances in botanical research. Eutectic solvents and stress in plants. Amsterdam, The Netherlands: Academic Press, 133–158.
Farrant JM, Hilhorst HWM. 2021. What is dry? Exploring metabolism and molecular mobility at extremely low water contents. Journal of Experimental Botany 72: 1507–1510.
Gallina A, Stocco N, Mutinelli F. 2010. Karl Fischer Titration to determine moisture in honey: a new simplified approach. Food Control 21: 942–944.
Gómez AV, Tadini CC, Biswas A, Buttrum M, Kim S, Boddu VM, Cheng HN. 2019. Microwave‐assisted extraction of soluble sugars from banana puree with natural deep eutectic solvents (NADES). LWT 107: 79–88.
Gravot A, Dittami SM, Rousvoal S, Lugan R, Eggert A, Collén J, Boyen C, Bouchereau A, Tonon T. 2010. Diurnal oscillations of metabolite abundances and gene analysis provide new insights into central metabolic processes of the brown alga Ectocarpus siliculosus. New Phytologist 188: 98–110.
He H, Gao H, Xue X, Ren J, Chen X, Niu B. 2024. Variation of sugar compounds in Phoebe chekiangensis seeds during natural desiccation. PLoS ONE 19: e0299669.
Hoekstra FA, Golovina EA, Buitink J. 2001. Mechanisms of plant desiccation tolerance. Trends in Plant Science 6: 431–438.
Ivanović M, Islamčević Razboršek M, Kolar M. 2020. Innovative extraction techniques using deep eutectic solvents and analytical methods for the isolation and characterization of natural bioactive compounds from plant material. Plants 9: 1428.
Khodaverdian S, Dabirmanesh B, Heydari A, Dashtban‐moghadam E, Khajeh K, Ghazi F. 2018. Activity, stability and structure of laccase in betaine based natural deep eutectic solvents. International Journal of Biological Macromolecules 107: 2574–2579.
Knudsen C, Bavishi K, Viborg KM, Drew DP, Simonsen HT, Motawia MS, Møller BL, Laursen T. 2020. Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent. Phytochemistry 170: 112214.
Leprince O, Buitink J. 2010. Desiccation tolerance: From genomics to the field. Plant Science 179: 554–564.
Li X, Zhuo J, Jing Y, Liu X, Wang X. 2011. Expression of a GALACTINOL SYNTHASE gene is positively associated with desiccation tolerance of Brassica napus seeds during development. Journal of Plant Physiology 168: 1761–1770.
Lindberg D, De La Fuente Revenga M, Widersten M. 2010. Deep eutectic solvents (DESs) are viable cosolvents for enzyme‐catalyzed epoxide hydrolysis. Journal of Biotechnology 147: 169–171.
Marks RA, Ekwealor JTB, Artur MAS, Bondi L, Boothby TC, Carmo OMS, Centeno DC, Coe KK, Dace HJW, Field S et al. 2025. Life on the dry side: a roadmap to understanding desiccation tolerance and accelerating translational applications. Nature Communications 16: 3284.
Martins MAR, Pinho SP, Coutinho JAP. 2019. Insights into the nature of eutectic and deep eutectic mixtures. Journal of Solution Chemistry 48: 962–982.
Matilla AJ. 2022. The orthodox dry seeds are alive: a clear example of desiccation tolerance. Plants 11: 20.
Møller BL, Laursen T. 2021. Chapter eight – Metabolons and bio‐condensates: the essence of plant plasticity and the key elements in development of green production systems. In: Verpoorte R, Witkamp G‐J, Choi YH, eds. Advances in botanical research. Eutectic solvents and stress in plants. Amsterdam, The Netherlands: Academic Press, 185–223.
Nicholson V, Meese E, Boothby TC. 2025. Osmolyte‐IDP interactions during desiccation. Progress in Molecular Biology and Translational Science 211: 39–61.
Percevault L, Delhaye T, Chaumont A, Schurhammer R, Paquin L, Rondeau D. 2021. Cold‐spray ionization mass spectrometry of the choline chloride‐urea deep eutectic solvent (reline). Journal of Mass Spectrometry 56: e4725.
Renault H, Roussel V, El Amrani A, Arzel M, Renault D, Bouchereau A, Deleu C. 2010. The Arabidopsis pop2‐1mutant reveals the involvement of GABA transaminase in salt stress tolerance. BMC Plant Biology 10: 20.
Rolletschek H, Mayer S, Boughton B, Wagner S, Ortleb S, Kiel C, Roessner U, Borisjuk L. 2021. The metabolic environment of the developing embryo: A multidisciplinary approach on oilseed rapeseed. Journal of Plant Physiology 265: 153505.
Roszkowska M, Gołdyn B, Wojciechowska D, Księżkiewicz Z, Fiałkowska E, Pluskota M, Kmita H, Kaczmarek Ł. 2023. How long can tardigrades survive in the anhydrobiotic state? A search for tardigrade Anhydrobiosis patterns. PLoS ONE 18: e0270386.
Smith EL, Abbott AP, Ryder KS. 2014. Deep eutectic solvents (DESs) and their applications. Chemical Reviews 114: 11060–11082.
du Toit SF, Bentley J, Farrant JM. 2021. Chapter nine – NADES formation in vegetative desiccation tolerance: Prospects and challenges. In: Verpoorte R, Witkamp G‐J, Choi YH, eds. Advances in botanical research. Eutectic solvents and stress in plants. Amsterdam, The Netherlands: Academic Press, 225–252.
VanBuren R, Wai CM, Ou S, Pardo J, Bryant D, Jiang N, Mockler TC, Edger P, Michael TP. 2018. Extreme haplotype variation in the desiccation‐tolerant clubmoss Selaginella lepidophylla. Nature Communications 9: 13.
Vanda H, Mustafa NR, Verpoorte R, Klinkhamer PGL, Choi YH. 2021. Chapter ten – Natural deep eutectic solvents present in plant exudates? A case study on the saps of Drosera species. In: Verpoorte R, Witkamp G‐J, Choi YH, eds. Advances in botanical research. Eutectic solvents and stress in plants. Amsterdam, The Netherlands: Academic Press, 253–269.
Verdier J, Lalanne D, Pelletier S, Torres‐Jerez I, Righetti K, Bandyopadhyay K, Leprince O, Chatelain E, Vu BL, Gouzy J et al. 2013. A regulatory network‐based approach dissects late maturation processes related to the acquisition of desiccation tolerance and longevity of Medicago truncatula seeds. Plant Physiology 163: 757–774.
Walters C, Ballesteros D, Vertucci VA. 2010. Structural mechanics of seed deterioration: standing the test of time. Plant Science 179: 565–573.
Wang W‐Q, Liu S‐J, Song S‐Q, Møller IM. 2015. Proteomics of seed development, desiccation tolerance, germination and vigor. Plant Physiology and Biochemistry 86: 1–15.
Yamaguchi K. 2003. Cold‐spray ionization mass spectrometry: principle and applications. Journal of Mass Spectrometry 38: 473–490.
Yamaguchi K. 2013. Cold‐spray ionization mass spectrometry: applications in structural coordination chemistry. Mass Spectrometry 2: S0012.
You X, Lee E, Xu C, Baiz CR. 2021. Molecular mechanism of cell membrane protection by sugars: a study of interfacial H‐bond networks. Journal of Physical Chemistry Letters 12: 9602–9607.
Grant Information: Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement; Centre National de la Recherche Scientifique; Université de Rennes; Région Bretagne
Contributed Indexing: Keywords: DESmolytes; NaDES; dehydration; desiccation tolerance; molecular association; oilseed rape (Brassica napusL.); seeds; vitrification
Substance Nomenclature: 57-50-1 (Sucrose)
059QF0KO0R (Water)
0 (Amino Acids)
Entry Date(s): Date Created: 20260312 Date Completed: 20260702 Latest Revision: 20260726
Update Code: 20260726
PubMed Central ID: PMC13326504
DOI: 10.1111/nph.71080
PMID: 41820017
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1469-8137
DOI:10.1111/nph.71080