Academic Journal
Nanozymes on paper: Transforming microfluidic devices for portable biosensing.
| Title: | Nanozymes on paper: Transforming microfluidic devices for portable biosensing. |
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| Authors: | Zhao J; Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Functional Proteins, College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China., Luo E; School of Chemistry and Chemical Engineering, Shanxi Normal University, Taiyuan, 030032, China., Wang W; College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China., Du Z; Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Functional Proteins, College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China. Electronic address: duzhi@tyut.edu.cn., Huang D; Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Functional Proteins, College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China. Electronic address: huangjw2067@163.com. |
| Source: | Talanta [Talanta] 2026 Aug 01; Vol. 305, pp. 129646. Date of Electronic Publication: 2026 Mar 07. |
| Publication Type: | Journal Article; Review |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| MeSH Terms: | Biosensing Techniques*/instrumentation , Biosensing Techniques*/methods , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods , Nanostructures*/chemistry , Paper* , Lab-On-A-Chip Devices*, Biomarkers/analysis ; Humans ; Rapid Diagnostic Tests ; Point-of-Care Systems |
| Abstract: | Paper-based microfluidic analytical devices (μPADs) form a hydrophilic channel by patterning a hydrophobic barrier, and use capillary action to achieve pumpless fluid control. As point-of-care testing (POCT) platforms, they have significant advantages such as low cost, ease of operation, and low sample consumption compared with traditional microfluidic chips. They are especially suitable for biomarker detection in resource-limited areas and home healthcare settings. To overcome the high cost and environmental instability of traditional natural enzymes as biorecognition elements, redox nanozymes with peroxidase (POD)-like activity have become a stable and economical alternative. Through the synergistic combination of physical adsorption and in situ growth techniques, nanozyme-μPAD constructs offer expedited, user-friendly detection capabilities for a broad spectrum of analytes, encompassing disease biomarkers, pathogenic agents, and environmental pollutants. This article reviews the research progress of the functional transformation of μPADs through the integration of nanozymes, systematically discusses the categories, design, screening, and regulation strategies of nanozymes, and elaborates on their catalytic mechanism in signal amplification. The article focuses on the key applications of nanozyme-functionalized μPADs in the fields of biomedical diagnosis and food safety detection, and outlines future development trends, existing challenges, and potential research directions of this technology. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Bioanalysis; Biosensor; Nanozyme; Non-enzymatic detection; Paper-based microfluidic analytical devices (μPADs); Point-of-care testing (POCT) |
| Substance Nomenclature: | 0 (Biomarkers) |
| Entry Date(s): | Date Created: 20260312 Date Completed: 20260711 Latest Revision: 20260711 |
| Update Code: | 20260711 |
| DOI: | 10.1016/j.talanta.2026.129646 |
| PMID: | 41819714 |
| Database: | MEDLINE |
| ISSN: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2026.129646 |