Academic Journal
A Sensitive Sandwich-type Electrochemical Immunosensor Based on Signal Amplification Strategy of Hollow CuS Nanocomposites for BNP Detection.
| Τίτλος: | A Sensitive Sandwich-type Electrochemical Immunosensor Based on Signal Amplification Strategy of Hollow CuS Nanocomposites for BNP Detection. |
|---|---|
| Συγγραφείς: | Zou L; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China., Hou J; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China., Shi Z; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China., Yin G; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China., Zhao H; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China. Electronic address: honglizhao@ecust.edu.cn., Lan M; Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China; Research Center of Analysis and Test, East China University of Science and Technology, Shanghai, 200237, People's Republic of China. Electronic address: minbolan@ecust.edu.cn. |
| Πηγή: | Talanta [Talanta] 2026 Aug 01; Vol. 305, pp. 129628. Date of Electronic Publication: 2026 Mar 04. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| Ιατρικοί όροι (MeSH): | Natriuretic Peptide, Brain*/blood , Nanocomposites*/chemistry , Electrochemical Techniques*/methods , Copper*/chemistry , Biosensing Techniques*/methods , Heart Failure*/diagnosis , Heart Failure*/blood, Gold/chemistry ; Metal Nanoparticles/chemistry ; Immunoassay/methods ; Graphite/chemistry ; Nanotubes, Carbon/chemistry ; Humans ; Animals ; Reproducibility of Results ; Limit of Detection ; Mice |
| Περίληψη: | Timely diagnosis and prognosis evaluation of heart failure (HF) urgently require highly sensitive, rapid, and reliable quantification of the critical biomarkers such as B-type natriuretic peptide (BNP). In this work, a novel sandwich-type electrochemical immunosensor was successfully constructed for highly sensitive and selective detection of BNP. Firstly, gold nanoparticles (AuNPs) and nitrogen-doped reduced graphene oxide/carboxylated multi-walled carbon nanotubes (N-rGO/CMWCNTs) were modified on the electrode surface for efficient capture antibody immobilization. Simultaneously, a core-shell structured HCuS@PDA@Pt nanocomposite was synthesized as a catalytic label. Quantification of BNP was achieved through specific immunorecognition and subsequent catalytic signal amplification. The proposed immunosensor demonstrated a wide linear detection range (1 pg mL-1-100 ng mL-1) and an ultra-low detection limit (0.25 pg mL-1) for BNP, along with superior selectivity, reproducibility, and stability. Especially, the biosensor exhibited excellent consistency with a commercial ELISA kit when analyzing real human serum samples. Furthermore, in a hypoxia-induced HF mice model, significantly elevated BNP levels due to cardiac stress were detected, confirming its ability to clearly distinguish between pathological and physiological states. The results validate the accuracy and reliability of the proposed biosensor for BNP detection in complex biological environments and highlight its promise for clinical diagnosis and monitoring applications. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: B-type natriuretic peptide; Pt nanoparticles; Sandwich-type; electrochemical immunosensor; hollow copper sulfide |
| Substance Nomenclature: | 114471-18-0 (Natriuretic Peptide, Brain) 789U1901C5 (Copper) 7440-57-5 (Gold) 7782-42-5 (Graphite) KL4YU612X7 (cupric sulfide) 0 (Nanotubes, Carbon) |
| Entry Date(s): | Date Created: 20260311 Date Completed: 20260710 Latest Revision: 20260710 |
| Update Code: | 20260711 |
| DOI: | 10.1016/j.talanta.2026.129628 |
| PMID: | 41812404 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-3573 |
|---|---|
| DOI: | 10.1016/j.talanta.2026.129628 |