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Rapid in-situ enrichment of anammox bacteria in a thiosulfate-driven partial denitrification and anammox biofilter packed with iron sponge.

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Τίτλος: Rapid in-situ enrichment of anammox bacteria in a thiosulfate-driven partial denitrification and anammox biofilter packed with iron sponge.
Συγγραφείς: Gu Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China., Zeng W; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: zengwei@bjut.edu.cn., Miao H; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China., Peng Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
Πηγή: Bioresource technology [Bioresour Technol] 2026 Jun; Vol. 449, pp. 134359. Date of Electronic Publication: 2026 Mar 06.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s): Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
Ιατρικοί όροι (MeSH): Denitrification*/drug effects , Thiosulfates*/pharmacology , Anaerobic Ammonia Oxidation*/drug effects, Sewage/microbiology ; Iron/chemistry ; Nitrogen/chemistry ; Nitrogen/isolation & purification ; Nitrogen/metabolism ; Bacteria/drug effects ; Bacteria/metabolism ; Heme/analogs & derivatives ; Oxidation-Reduction
Περίληψη: Despite its high efficiency and low-carbon advantages, the engineering application of anaerobic ammonium oxidation (anammox) technology has long been constrained by two major bottlenecks: slow start-up and limited sources of microbial inoculum. This study established a thiosulfate-driven partial denitrification/anammox biofilter (TDDA-BF) system, achieving rapid start-up using conventional activated sludge and sulfur‑autotrophic denitrifying sludge, without the need for external anammox inoculation. The TDDA-BF system successfully achieved in-situ enrichment of anammox bacteria within 78 days, with a total nitrogen removal efficiency of 85%. Microbial community analysis revealed a substantial increase in the abundance of Candidatus_Brocadia from 0.38% to 21.01%. Hydrazine dehydrogenase activity assays and predictions suggested that Fe (II) from iron sponge boosted anammox key enzyme activity by enhancing heme c synthesis. This study successfully demonstrates the feasibility of rapid in-situ enrichment of anammox bacteria in a TDDA-BF system. The implementation of this strategy not only substantially shortens the conventional start-up period but also promotes a more carbon-efficient pathway for nitrogen removal, highlighting its potential for sustainable wastewater treatment applications.
(Copyright © 2026. Published by Elsevier Ltd.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Anammox; In-situ self-enrichment; Iron sponge; Microbial community; Nitrogen removal mechanism; Thiosulfate-driven partial denitrification andanammox
Substance Nomenclature: 0 (Thiosulfates)
0 (Sewage)
E1UOL152H7 (Iron)
26598-29-8 (heme C)
N762921K75 (Nitrogen)
42VZT0U6YR (Heme)
Entry Date(s): Date Created: 20260309 Date Completed: 20260807 Latest Revision: 20260807
Update Code: 20260807
DOI: 10.1016/j.biortech.2026.134359
PMID: 41796810
Βάση Δεδομένων: MEDLINE
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  Data: Rapid in-situ enrichment of anammox bacteria in a thiosulfate-driven partial denitrification and anammox biofilter packed with iron sponge.
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  Data: <searchLink fieldCode="AU" term="%22Gu+Y%22">Gu Y</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.<br /><searchLink fieldCode="AU" term="%22Zeng+W%22">Zeng W</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: zengwei@bjut.edu.cn.<br /><searchLink fieldCode="AU" term="%22Miao+H%22">Miao H</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.<br /><searchLink fieldCode="AU" term="%22Peng+Y%22">Peng Y</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
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  Data: <searchLink fieldCode="MM" term="%22Denitrification%22">Denitrification*</searchLink>/<searchLink fieldCode="MM" term="%22Denitrification+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Thiosulfates%22">Thiosulfates*</searchLink>/<searchLink fieldCode="MM" term="%22Thiosulfates+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Anaerobic+Ammonia+Oxidation%22">Anaerobic Ammonia Oxidation*</searchLink>/<searchLink fieldCode="MM" term="%22Anaerobic+Ammonia+Oxidation+drug+effects%22">drug effects</searchLink><br /><searchLink fieldCode="MH" term="%22Sewage%22">Sewage</searchLink>/<searchLink fieldCode="MH" term="%22Sewage+microbiology%22">microbiology</searchLink> ; <searchLink fieldCode="MH" term="%22Iron%22">Iron</searchLink>/<searchLink fieldCode="MH" term="%22Iron+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrogen%22">Nitrogen</searchLink>/<searchLink fieldCode="MH" term="%22Nitrogen+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrogen%22">Nitrogen</searchLink>/<searchLink fieldCode="MH" term="%22Nitrogen+isolation+%26+purification%22">isolation & purification</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrogen%22">Nitrogen</searchLink>/<searchLink fieldCode="MH" term="%22Nitrogen+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Heme%22">Heme</searchLink>/<searchLink fieldCode="MH" term="%22Heme+analogs+%26+derivatives%22">analogs & derivatives</searchLink> ; <searchLink fieldCode="MH" term="%22Oxidation-Reduction%22">Oxidation-Reduction</searchLink>
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  Data: Despite its high efficiency and low-carbon advantages, the engineering application of anaerobic ammonium oxidation (anammox) technology has long been constrained by two major bottlenecks: slow start-up and limited sources of microbial inoculum. This study established a thiosulfate-driven partial denitrification/anammox biofilter (TDDA-BF) system, achieving rapid start-up using conventional activated sludge and sulfur‑autotrophic denitrifying sludge, without the need for external anammox inoculation. The TDDA-BF system successfully achieved in-situ enrichment of anammox bacteria within 78 days, with a total nitrogen removal efficiency of 85%. Microbial community analysis revealed a substantial increase in the abundance of Candidatus_Brocadia from 0.38% to 21.01%. Hydrazine dehydrogenase activity assays and predictions suggested that Fe (II) from iron sponge boosted anammox key enzyme activity by enhancing heme c synthesis. This study successfully demonstrates the feasibility of rapid in-situ enrichment of anammox bacteria in a TDDA-BF system. The implementation of this strategy not only substantially shortens the conventional start-up period but also promotes a more carbon-efficient pathway for nitrogen removal, highlighting its potential for sustainable wastewater treatment applications.<br /> (Copyright © 2026. Published by Elsevier Ltd.)
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  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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  Data: <i>Keywords: </i>Anammox; In-situ self-enrichment; Iron sponge; Microbial community; Nitrogen removal mechanism; Thiosulfate-driven partial denitrification andanammox
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  Data: 0 (Thiosulfates)<br />0 (Sewage)<br />E1UOL152H7 (Iron)<br />26598-29-8 (heme C)<br />N762921K75 (Nitrogen)<br />42VZT0U6YR (Heme)
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        Value: 10.1016/j.biortech.2026.134359
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        Text: English
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        Type: general
      – SubjectFull: Iron chemistry
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      – TitleFull: Rapid in-situ enrichment of anammox bacteria in a thiosulfate-driven partial denitrification and anammox biofilter packed with iron sponge.
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