Academic Journal
Rapid in-situ enrichment of anammox bacteria in a thiosulfate-driven partial denitrification and anammox biofilter packed with iron sponge.
| Τίτλος: | Rapid in-situ enrichment of anammox bacteria in a thiosulfate-driven partial denitrification and anammox biofilter packed with iron sponge. |
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| Συγγραφείς: | Gu Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China., Zeng W; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: zengwei@bjut.edu.cn., Miao H; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China., Peng Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Jun; Vol. 449, pp. 134359. Date of Electronic Publication: 2026 Mar 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Denitrification*/drug effects , Thiosulfates*/pharmacology , Anaerobic Ammonia Oxidation*/drug effects, Sewage/microbiology ; Iron/chemistry ; Nitrogen/chemistry ; Nitrogen/isolation & purification ; Nitrogen/metabolism ; Bacteria/drug effects ; Bacteria/metabolism ; Heme/analogs & derivatives ; Oxidation-Reduction |
| Περίληψη: | Despite its high efficiency and low-carbon advantages, the engineering application of anaerobic ammonium oxidation (anammox) technology has long been constrained by two major bottlenecks: slow start-up and limited sources of microbial inoculum. This study established a thiosulfate-driven partial denitrification/anammox biofilter (TDDA-BF) system, achieving rapid start-up using conventional activated sludge and sulfur‑autotrophic denitrifying sludge, without the need for external anammox inoculation. The TDDA-BF system successfully achieved in-situ enrichment of anammox bacteria within 78 days, with a total nitrogen removal efficiency of 85%. Microbial community analysis revealed a substantial increase in the abundance of Candidatus_Brocadia from 0.38% to 21.01%. Hydrazine dehydrogenase activity assays and predictions suggested that Fe (II) from iron sponge boosted anammox key enzyme activity by enhancing heme c synthesis. This study successfully demonstrates the feasibility of rapid in-situ enrichment of anammox bacteria in a TDDA-BF system. The implementation of this strategy not only substantially shortens the conventional start-up period but also promotes a more carbon-efficient pathway for nitrogen removal, highlighting its potential for sustainable wastewater treatment applications. (Copyright © 2026. Published by Elsevier Ltd.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Anammox; In-situ self-enrichment; Iron sponge; Microbial community; Nitrogen removal mechanism; Thiosulfate-driven partial denitrification andanammox |
| Substance Nomenclature: | 0 (Thiosulfates) 0 (Sewage) E1UOL152H7 (Iron) 26598-29-8 (heme C) N762921K75 (Nitrogen) 42VZT0U6YR (Heme) |
| Entry Date(s): | Date Created: 20260309 Date Completed: 20260807 Latest Revision: 20260807 |
| Update Code: | 20260807 |
| DOI: | 10.1016/j.biortech.2026.134359 |
| PMID: | 41796810 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2026.134359 |