Academic Journal
Microbial-induced calcite precipitation by indigenous alkaliphilic bacteria: a dual-enzyme strategy for crack-healing in cementitious materials.
| Title: | Microbial-induced calcite precipitation by indigenous alkaliphilic bacteria: a dual-enzyme strategy for crack-healing in cementitious materials. |
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| Authors: | Shiri M; Department of Civil Engineering, University of Zanjan, Zanjan, Iran., Bahari A; Research Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran. Bahari@znu.ac.ir., Sarbangholi MMK; Department of Civil Engineering, University of Zanjan, Zanjan, Iran., IvanovichVatin N; Advanced Engineering School for Digital Engineering, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia., Rahmani H; Department of Civil Engineering, University of Zanjan, Zanjan, Iran., Bozorg AE; Department of Civil Engineering, University of Zanjan, Zanjan, Iran., Gholamian E; Research Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran., Kordlou M; Research Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran. |
| Source: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2026 Mar 06; Vol. 110 (1). Date of Electronic Publication: 2026 Mar 06. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin ; New York : Springer International, c1984- |
| MeSH Terms: | Calcium Carbonate*/metabolism , Calcium Carbonate*/chemistry , Urease*/metabolism , Construction Materials*/microbiology , Carbonic Anhydrases*/metabolism , Bacteria*/enzymology , Bacteria*/metabolism , Bacteria*/isolation & purification, Sporosarcina/enzymology ; Sporosarcina/metabolism ; Sporosarcina/isolation & purification ; Bacillus subtilis/enzymology ; Bacillus subtilis/metabolism ; Bacillus subtilis/isolation & purification ; Bacillus/enzymology ; Bacillus/metabolism ; Hydrogen-Ion Concentration ; Chemical Precipitation ; Bacillaceae |
| Abstract: | Microbial-induced calcite precipitation (MICP) offers a sustainable strategy for extending the service life of concrete through autonomous crack healing, yet the high alkalinity of cementitious environments restricts microbial viability. In this study, more than 200 indigenous bacterial isolates collected from extreme environments across Iran were systematically screened for urease and carbonic anhydrase (CA) activities. A dual-enzyme activity index (EAI) was developed to quantitatively rank their calcification potential. Four robust spore-forming strains-Bacillus subtilis, Sporosarcina pasteurii, Bacillus sphaericus, and the environmental isolate E10.2-were identified as top candidates based on high EAI values, sporulation capacity, and survival at pH 13.5. These strains retained at least 70% of their enzymatic activity after alkaline exposure and precipitated up to 89% more CaCO (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Contributed Indexing: | Keywords: Alkaliphilic spore-forming bacteria; Carbonic anhydrase activity; MICP; Native microbial isolates; Self-healing concrete; Urease activity |
| Substance Nomenclature: | H0G9379FGK (Calcium Carbonate) EC 3.5.1.5 (Urease) EC 4.2.1.1 (Carbonic Anhydrases) |
| SCR Organism: | Sporosarcina pasteurii; Lysinibacillus sphaericus |
| Entry Date(s): | Date Created: 20260306 Date Completed: 20260710 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC12967677 |
| DOI: | 10.1007/s00253-026-13764-7 |
| PMID: | 41792256 |
| Database: | MEDLINE |
| ISSN: | 1432-0614 |
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| DOI: | 10.1007/s00253-026-13764-7 |