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Protective effect of alpha-tocopherol succinate on Al₂O₃-NPs induced damage in NMRI mice Sertoli cells: the role of inhibin B and Connexin 43.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Protective effect of alpha-tocopherol succinate on Al₂O₃-NPs induced damage in NMRI mice Sertoli cells: the role of inhibin B and Connexin 43.
Συγγραφείς: Naki MD; Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran., Amini E; Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. elaheh.amini@khu.ac.ir., Rohollah F; Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Πηγή: Molecular biology reports [Mol Biol Rep] 2026 Mar 03; Vol. 53 (1). Date of Electronic Publication: 2026 Mar 03.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
Imprint Name(s): Original Publication: Dordrecht, Boston, Reidel.
Ιατρικοί όροι (MeSH): Connexin 43*/metabolism , Connexin 43*/genetics , alpha-Tocopherol*/pharmacology , alpha-Tocopherol*/metabolism , Sertoli Cells*/drug effects , Sertoli Cells*/metabolism , Inhibins*/metabolism , Inhibins*/genetics , Aluminum Oxide*/toxicity, Apoptosis/drug effects ; Oxidative Stress/drug effects ; Cell Survival/drug effects ; Spermatogenesis/drug effects ; Vitamin E/pharmacology ; Antioxidants/pharmacology ; Animals ; Male ; Mice
Περίληψη: BACKGROUND: Sertoli cells are essential for spermatogenesis, controlling the microenvironment and interacting with germ cells. This study evaluates the preventive effect of alpha-tocopherol succinate against Al₂O₃-NPs-induced damage in NMRI mice Sertoli cells. METHODS: The effects of Al₂O₃-NPs and vitamin E were assessed on Sertoli cells using viability assays, oxidative stress markers, apoptosis detection, and gene expression analysis. RESULTS: Al₂O₃-NPs significantly reduced Sertoli cell viability and increased apoptosis, while vitamin E treatment mitigated these effects. Expression of INHBB and Cx43 was significantly downregulated by Al₂O₃-NPs and upregulated with vitamin E. CONCLUSION: Vitamin E alleviates Al₂O₃-NPs-induced Sertoli cell damage by reducing oxidative stress, inhibiting apoptosis, and restoring INHBB and Cx43 expression.
Competing Interests: Declarations. Conflict of interest: All authors declare that they have no conflicts of interest and agree with the content of the manuscript. Ethical approval: All animal procedures in this study commenced after ethical acceptance secured from the Ethics Committee of Islamic Azad University of Tehran, Iran, with ethical code (IR.IAU.PS.REC.1402.557).
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Contributed Indexing: Keywords: Alumina nanoparticles; Apoptosis; Connexin 43; Sertoli cells; alpha-tocopherol succinate; inhibin B
Substance Nomenclature: 0 (Connexin 43)
H4N855PNZ1 (alpha-Tocopherol)
57285-09-3 (Inhibins)
0 (inhibin B)
LMI26O6933 (Aluminum Oxide)
1406-18-4 (Vitamin E)
0 (Antioxidants)
Entry Date(s): Date Created: 20260303 Date Completed: 20260627 Latest Revision: 20260627
Update Code: 20260628
DOI: 10.1007/s11033-026-11566-8
PMID: 41774233
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1573-4978
DOI:10.1007/s11033-026-11566-8