Deciphering the mechanisms underlying cadmium tolerance in the endophytic fungus Trichoderma atroviride D16.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Deciphering the mechanisms underlying cadmium tolerance in the endophytic fungus Trichoderma atroviride D16.
Συγγραφείς: Bi XW; School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 311402, Zhejiang, PR China., Xie XG; School of Pharmacy, Naval Medical University, Shanghai, 200433, PR China. Electronic address: xgxie@smmu.edu.cn., Zhang WJ; School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 311402, Zhejiang, PR China., Zhang RD; School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 311402, Zhejiang, PR China., Rahman K; Faculty of Science, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK., Yu XZ; School of Pharmacy, Naval Medical University, Shanghai, 200433, PR China., Fan CY; School of Pharmacy, Naval Medical University, Shanghai, 200433, PR China., Ding SL; School of Pharmacy, Naval Medical University, Shanghai, 200433, PR China., Peng YQ; School of Pharmacy, Naval Medical University, Shanghai, 200433, PR China., Han T; School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 311402, Zhejiang, PR China; School of Pharmacy, Naval Medical University, Shanghai, 200433, PR China. Electronic address: hanting@smmu.edu.cn.
Πηγή: Fungal biology [Fungal Biol] 2026 Apr; Vol. 130 (2), pp. 101732. Date of Electronic Publication: 2026 Feb 04.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Published by Elsevier, copyright by British Mycological Society Country of Publication: Netherlands NLM ID: 101524465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-6146 (Print) NLM ISO Abbreviation: Fungal Biol Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Published by Elsevier, copyright by British Mycological Society, c2010-
Ιατρικοί όροι (MeSH): Cadmium*/metabolism , Cadmium*/toxicity , Endophytes*/metabolism , Endophytes*/drug effects , Hypocreales*/drug effects , Hypocreales*/metabolism , Hypocreales*/genetics , Hypocreales*/growth & development, Drug Tolerance ; Gene Expression Regulation, Fungal
Περίληψη: Human activities have caused substantial heavy metal contamination, with cadmium (Cd(II)) presenting a notable hazard due to its persistence and tendency to accumulate in living organisms, leading to severe toxicity across various ecological levels. This research investigates the tolerance of the endophytic fungus Trichoderma atroviride D16, derived from Salvia miltiorrhiza, to Cd(II) as a promising and economical approach for Cd(II) mitigation. At exogenous Cd(II) concentrations of ≥150 mg L-1, the colony growth diameter of D16 showed little change, with a tolerance index consistently maintained between 0.82 and 0.93 on the fifth day of cultivation. The Cd(II) tolerance EC50 (half-maximal effective concentration) concentration of D16 was determined to be 47.21 mg L-1 in liquid culture. Concurrently, the strain's Cd(II) adsorption efficiency was between 20.9% and 37.6%. Under elevated Cd(II) concentrations, D16 exhibited significant upregulation of superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione (GSH) actively contributing to Cd(II) detoxification. Transcriptome analysis identified three primary response pathways to Cd(II) stress: transmembrane transporter, chelation, and regulation of antioxidant enzyme activity. Genes related to cadmium transporter, glutathione synthesis, and antioxidant enzymes were notably upregulated at 50 mg L-1 Cd(II), indicating that gene expression regulation is crucial for D16's high Cd(II) tolerance. In Arabidopsis thaliana exposed to 50 mg kg-1 Cd(II), D16 inoculation significantly enhanced biomass and reduced Cd(II) accumulation. Mechanistically, D16 mitigated stress by enhancing host antioxidant activity and regulating key Cd-responsive genes to restrict Cd uptake and promote detoxification. In conclusion, D16 possesses robust self-resistance and actively fortifies host plant tolerance, offering essential microbial resources and theoretical support for the bioremediation of Cd(II) contamination.
(Copyright © 2026. Published by Elsevier Ltd.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Antioxidant activity; Cadmium; Glutathione; Transcriptome; Trichoderma atroviride D16
Molecular Sequence: BioProject PRJNA1320983
Substance Nomenclature: 00BH33GNGH (Cadmium)
SCR Organism: Trichoderma atroviride
Entry Date(s): Date Created: 20260301 Date Completed: 20260709 Latest Revision: 20260711
Update Code: 20260712
DOI: 10.1016/j.funbio.2026.101732
PMID: 41765615
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1878-6146
DOI:10.1016/j.funbio.2026.101732