Academic Journal
Canonical human blood dendritic cells are distinguished by robust E-selectin binding.
| Τίτλος: | Canonical human blood dendritic cells are distinguished by robust E-selectin binding. |
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| Συγγραφείς: | Ales E; Department of Translational Medicine and Translational Glycobiology Institute, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, United States., Sackstein R; Department of Translational Medicine and Translational Glycobiology Institute, Herbert Wertheim College of Medicine, Florida International University, Miami, FL, United States. |
| Πηγή: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2026 Feb 09; Vol. 215 (2). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Association of Immunologists Country of Publication: England NLM ID: 2985117R Publication Model: Print Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Bethesda, MD : American Association of Immunologists Original Publication: Baltimore : Williams & Wilkins, c1950- |
| Ιατρικοί όροι (MeSH): | E-Selectin*/metabolism , E-Selectin*/immunology , Dendritic Cells*/immunology , Dendritic Cells*/metabolism, Sialyl Lewis X Antigen/metabolism ; Humans ; Protein Binding |
| Περίληψη: | Human blood leukocytes continually flux between blood and tissues. The first step in extravasation of blood-borne cells involves shear-resistant binding to the endothelial surface, a process mediated by adhesive interactions between an endothelial lectin, E-selectin, and a tetrasaccharide known as sialyl Lewis X (sLeX) displayed on circulating cells. Human neutrophils and monocytes express high levels of "E-selectin ligands" (glycoconjugates that contain sLeX) and are thereby operationally primed to extravasate at inflammatory loci. However, the capacity of human blood dendritic cells (DCs) to similarly engage E-selectin is unknown. To address this question, we performed multiparameter flow cytometry, biochemical analyses, and measurements of transcripts encoding key glycosyltransferases programming sLeX biosynthesis. Our results reveal that a unifying feature of all subsets of human blood DCs is the display of carbohydrates that endow robust E-selectin binding. This feature is also characteristic of their ontogenic precursors. Notably, while bulk RNA sequencing analysis is mechanistically informative, single-cell RNA sequencing does not provide insights into the critical chemoenzymatic effectors of sLeX expression by DCs. These results thus reconceptualize our understanding of an entire class of human immune cells that converge the innate and adaptive immune systems, providing key insights into the molecular basis of their tissue recruitment in both steady-state and at the onset of an inflammatory response. (© The Author(s) 2026. Published by Oxford University Press on behalf of The American Association of Immunologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| Grant Information: | U01 CA225730 United States CA NCI NIH HHS; NCI "Alliance of Glycobiologists for Cancer Research |
| Contributed Indexing: | Keywords: E-selectin; E-selectin ligand; dendritic cell; immunosurveillance; translational glycobiology |
| Substance Nomenclature: | 0 (E-Selectin) 0 (Sialyl Lewis X Antigen) |
| Entry Date(s): | Date Created: 20260301 Date Completed: 20260709 Latest Revision: 20260709 |
| Update Code: | 20260711 |
| DOI: | 10.1093/jimmun/vkaf307 |
| PMID: | 41764722 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1550-6606 |
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| DOI: | 10.1093/jimmun/vkaf307 |