Integrated Genetic Profiling for Cattle Parentage Testing via Next-Generation Sequencing.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Integrated Genetic Profiling for Cattle Parentage Testing via Next-Generation Sequencing.
Συγγραφείς:	Geng J; Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou, China., Liu J; Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou, China., Liu Z; Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou, China., Liu Z; Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou, China., Bao C; Saihan District Branch of Hohhot Public Security Bureau, Hohhot, China., Yan X; Saihan District Branch of Hohhot Public Security Bureau, Hohhot, China., Wang X; Saihan District Branch of Hohhot Public Security Bureau, Hohhot, China., Wu Z; Saihan District Branch of Hohhot Public Security Bureau, Hohhot, China., Sun H; Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou, China., Wu R; Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.; Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Sun Yat-sen University, Guangzhou, China.
Πηγή:	Electrophoresis [Electrophoresis] 2026 Apr; Vol. 47 (4), pp. 339-352. Date of Electronic Publication: 2026 Feb 28.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE
Imprint Name(s):	Publication: : Weinheim : Wiley-VCH Original Publication: [Weinheim, Germany] : Verlag Chemie, [1980-
Ιατρικοί όροι (MeSH):	High-Throughput Nucleotide Sequencing/methods , Genotyping Techniques/methods , Genetic Profile*, Cattle/genetics ; Microsatellite Repeats/genetics ; Animals ; Male ; Polymorphism, Single Nucleotide ; Reproducibility of Results ; Paternity ; Female
Περίληψη:	Cattle are frequently involved in civil disputes and offenses. Currently, the paternity testing of cattle is mainly done by analysis of dinucleotide STR (di-STR) or SNPs. However, the di-STR marker usually produces many stutter artifacts that affect the accurate genotyping, and the polymorphic level of SNPs is not high enough. To overcome these limitations, we developed and validated a novel integrated next-generation sequencing (NGS) panel for cattle. It comprises 33 reported di-STRs and 30 newly identified tetranucleotide STRs (tetra-STRs), the mitochondrial displacement loop (D-loop) region, as well as cytochrome b (CYTB) and recombination-activating gene 1 (RAG1). The results showed a high genotyping success rate (99.6% on average) and balanced amplification efficiency across most STR loci. In addition, the panel's average genotyping consistency rate reached 96.4% between blood and hair samples. The total discrimination power and cumulative probability of exclusion for duos of this panel were 1-1.8 × 10^-13 and 1-1.7 × 10^-8, respectively. Application in paternity testing caseworks demonstrates its applicability and reliability for resolving paternity disputes. Overall, this study provided a cattle profiling NGS panel with a substantial advancement over current capillary electrophoresis (CE)-based systems, offering superior accuracy, multiplex capability, and efficiency for forensic genetic testing in cattle. (© 2026 Wiley‐VCH GmbH.)
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Grant Information:	2023A1515030223 Natural Science Foundation of Guangdong Province; 81801878 National Natural Science Foundation of China
Contributed Indexing:	Keywords: Bos taurus; STR; animal forensic genetics; individual identification; paternity testing
Entry Date(s):	Date Created: 20260228 Date Completed: 20260710 Latest Revision: 20260715
Update Code:	20260715
DOI:	10.1002/elps.70082
PMID:	41761921
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1522-2683
DOI:	10.1002/elps.70082