Academic Journal
[Force-regulation mechanism of E-selectin mediated adhesion and activation of MDA-MB-231 cells under fluid shear stress].
| Τίτλος: | [Force-regulation mechanism of E-selectin mediated adhesion and activation of MDA-MB-231 cells under fluid shear stress]. |
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| Συγγραφείς: | Zhong P; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, P. R. China., Fang Y; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, P. R. China., Wu J; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, P. R. China. |
| Πηγή: | Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi [Sheng Wu Yi Xue Gong Cheng Xue Za Zhi] 2026 Feb 25; Vol. 43 (1), pp. 97-105. |
| Τύπος έκδοσης: | English Abstract; Journal Article |
| Γλώσσα: | Chinese |
| Στοιχεία περιοδικού: | Publisher: Sichuan Sheng sheng wu yi xue gong cheng xue hui Country of Publication: China NLM ID: 9426398 Publication Model: Print Cited Medium: Print ISSN: 1001-5515 (Print) Linking ISSN: 10015515 NLM ISO Abbreviation: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi Subsets: MEDLINE |
| Imprint Name(s): | Publication: Chengdu : Sichuan Sheng sheng wu yi xue gong cheng xue hui Original Publication: Chengdu : Sichuan Sheng sheng wu yi xue gong cheng xue hui : Hua xi yi ke da xue : Chengdu ke ji da xue |
| Ιατρικοί όροι (MeSH): | Cell Adhesion*/physiology , E-Selectin*/metabolism , Breast Neoplasms*/pathology , Breast Neoplasms*/metabolism , Stress, Mechanical*, Integrin alpha5beta1/metabolism ; Calcium/metabolism ; Fibronectins/metabolism ; Hyaluronan Receptors/metabolism ; Humans ; MDA-MB-231 Cells ; Female ; Shear Strength |
| Περίληψη: | The adhesion of cancer cells to the vascular endothelium during hematogenous metastasis is a crucial first step, involving the interaction of multiple adhesion molecules between cancer cells and endothelial cells. Here, a parallel-plate flow chamber combined with fluorescence microscopy was used to observe the adhesion behavior and subsequent calcium response of MDA-MB-231 cells on different functionalized substrates under flows, revealing the underlying force-regulation mechanism by analyzing and extracting relevant characteristic parameters. Our results demonstrated that fluid shear stress positively regulated the rolling velocity of cells by affecting the dissociation rate constant of CD44/E-selectin, and rapidly activated integrin α5β1 at the sub-second level, slowing down the rolling velocity of cells, but not enough to firm adhesion. Force triggered the calcium response of MDA-MB-231 cells on E-selectin. Furthermore, the activated integrin α5β1 binding with fibronectin enhanced and quickened cellular calcium response with higher activation ratio and peak intensity, and shorter delay time. This study can deepen the understanding of the hematogenous metastasis process of breast cancer cells, and provide reference for relevant clinical treatment strategies and drug development. |
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| Contributed Indexing: | Keywords: CD44; Cell adhesion; E-selectin; Fluid shear stress; Integrin Local Abstract: [Publisher, Chinese] 血行转移过程中肿瘤细胞在血管壁的黏附是关键的第一步，涉及多种黏附分子的相互作用。本文采用平行平板流动腔系统结合荧光显微镜，实时观察MDA-MB-231细胞在不同功能化底板上的黏附行为和钙响应过程，提取特征参数以揭示E-选择素介导的细胞黏附和活化的力调控机制。研究结果表明流体剪应力通过影响CD44/E-选择素的解离速率常数正向调控细胞的滚动速度，并在亚秒水平内快速激活整合素α5β1，减缓细胞的滚动速度，但不足以稳定黏附细胞。流体剪应力是启动E-选择素介导的MDA-MB-231细胞钙响应的开关，激活的整合素α5β1结合纤连蛋白进一步加强、加快了细胞钙响应，使激活比率和峰值强度提高，大大缩短延迟时间。本研究结果可深化对乳腺癌细胞血行转移过程的理解，为相关临床治疗策略的制定和药物研发提供参考。. |
| Substance Nomenclature: | 0 (E-Selectin) 0 (Integrin alpha5beta1) SY7Q814VUP (Calcium) 0 (Fibronectins) 0 (Hyaluronan Receptors) |
| Entry Date(s): | Date Created: 20260227 Date Completed: 20260709 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC12948538 |
| DOI: | 10.7507/1001-5515.202507049 |
| PMID: | 41760208 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1001-5515 |
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| DOI: | 10.7507/1001-5515.202507049 |