Academic Journal
C-type natriuretic peptide pre-maturation culture affects cytoplasmic maturation in bovine oocytes†.
| Τίτλος: | C-type natriuretic peptide pre-maturation culture affects cytoplasmic maturation in bovine oocytes†. |
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| Συγγραφείς: | Girka E; School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA., Brewer A; School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA., Ming H; Department of Animal Sciences, University of Florida, Gainesville, Florida 32608, USA., Sheikh E; Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, USA., Gartia MR; Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, USA., Jiang Z; Department of Animal Sciences, University of Florida, Gainesville, Florida 32608, USA., Looney CR; Department of Animal Science, Division of Agriculture, University of Arkansas, Hope, Arkansas 71801, USA., Bondioli KR; School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA. |
| Πηγή: | Biology of reproduction [Biol Reprod] 2026 Jun 15; Vol. 114 (6), pp. 2037-2052. |
| Τύπος έκδοσης: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2017- : New York : Oxford University Press Original Publication: Champaign, Ill. : Society for the Study of Reproduction |
| Ιατρικοί όροι (MeSH): | Natriuretic Peptide, C-Type*/pharmacology , Oocytes*/drug effects , Oocytes*/physiology , In Vitro Oocyte Maturation Techniques*/veterinary , In Vitro Oocyte Maturation Techniques*/methods , Cytoplasm*/drug effects , Cytoplasm*/physiology, Animals ; Cattle ; Female ; Transcriptome |
| Περίληψη: | In vitro oocyte maturation (IVM) is a vital step for many assisted reproductive technologies including in vitro embryo production. Modulation of ribonucleic acid (RNA) transcripts, lipids, mitochondria, and other molecules during maturation influences development significantly. To improve current maturation systems, inclusion of pre-maturation culture has been investigated to delay resumption of meiosis and allow appropriate progression of cytoplasmic maturation using C-type natriuretic peptide (CNP). In this study, the transcriptome and molecular fingerprint of germinal vesicle, conventional IVM, and CNP-IVM oocytes were compared to identify transcripts and associated pathways altered by CNP treatment. There were similar pathways associated with upregulated transcripts in both types of mature oocytes involved with mitochondrial function and translation. Oocytes that were matured under conventional conditions contained transcripts associated with messenger RNA surveillance, Polycomb repressive complex, and reactive oxygen species. Oocytes matured with CNP treatment contained transcripts associated with cofactor biosynthesis, autophagy, apoptosis, arginine and proline metabolism, and mechanistic target of rapamycin (mTOR). Compared to immature oocytes, all mitochondrial-derived protein encoding genes were upregulated after conventional IVM but not after CNP-IVM. The molecular fingerprint of lipid droplets was also significantly altered in CNP-treated oocytes. Overall, results of these experiments have demonstrated measurable transcriptomic and molecular differences in CNP treated oocytes that may enhance oocyte and embryo quality. (© The Author(s) 2026. Published by Oxford University Press on behalf of the Society for the Study of Reproduction. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| Grant Information: | W4171 USDA National Institute of Food and Agriculture Multi State projects; W5171 USDA National Institute of Food and Agriculture Multi State projects; R01HD102533 NIH Eunice Kennedy Shriver National Institute of Child Health and Human Development; 2019-67016-29863 USDA National Institute of Food and Agriculture; R35GM150564 United States GM NIGMS NIH HHS |
| Contributed Indexing: | Keywords: C-type natriuretic peptide; Raman spectroscopy; bovine; cytoplasmic maturation; maturation; oocyte |
| Molecular Sequence: | GEO GSE322639 |
| Substance Nomenclature: | 127869-51-6 (Natriuretic Peptide, C-Type) |
| Entry Date(s): | Date Created: 20260227 Date Completed: 20260617 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13273294 |
| DOI: | 10.1093/biolre/ioag053 |
| PMID: | 41757495 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1529-7268 |
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| DOI: | 10.1093/biolre/ioag053 |