Academic Journal
Impacts of microchannel parameters on CLEAs immobilization.
| Τίτλος: | Impacts of microchannel parameters on CLEAs immobilization. |
|---|---|
| Συγγραφείς: | Liu C; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China., Yin Z; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China., Ma C; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China., Wang S; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: wangsg@buct.edu.cn. |
| Πηγή: | Enzyme and microbial technology [Enzyme Microb Technol] 2026 Jun; Vol. 197, pp. 110835. Date of Electronic Publication: 2026 Feb 23. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: United States NLM ID: 8003761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0909 (Electronic) Linking ISSN: 01410229 NLM ISO Abbreviation: Enzyme Microb Technol Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York, NY : Elsevier Original Publication: [Guildford, Eng.] IPC Science and Technology Press. |
| Ιατρικοί όροι (MeSH): | Enzymes, Immobilized*/metabolism , Enzymes, Immobilized*/chemistry , Urease*/metabolism , Urease*/chemistry, Cross-Linking Reagents/chemistry ; Bioreactors ; Hydrodynamics ; Particle Size ; Protein Aggregates ; Continuous Flow Chemistry |
| Περίληψη: | Cross-linked enzyme aggregates (CLEAs) are suitable to use in microchemical technology, but CLEAs shortcomings limit their application. The combination of CLEAs and microchannel can overcome CLEAs shortcomings. However, it is unclear how CLEAs immobilization is affected by microchannel parameters. The effects of microchannel (geometry, diameter, length) and its groove (shape, depth, size, density) on urease-CLEAs immobilization were investigated. Moreover, CFD simulation was conducted to reveal the effects of CLEAs particle size on fluid dynamic in microchannel. Results showed that microchannel reactor with immobilized urease-CLEAs (MRIC) had the highest activity under the following optimal conditions: Wave-type microchannel, 50.0 cm in microchannel length, 2.0 mm in microchannel diameter, and triangular groove that was 1.0 mm in depth, 1.2 mm2 in size, and 2.0 individuals/cm2 in density. MRIC activity was up to 1.450 U/cm2, which was 4.63 times higher than that of the microchannel reactor with free urease (MRFU). Moreover, MRIC showed better reusability than MRFU. CFD simulation confirmed that the presence of CLEAs disturbed local flow velocity in microchannel. This study indicates that the combination of CLEAs and microchannel is a promising strategy to prepare enzymatic microchannel reactor. (Copyright © 2026 Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Cross-linked enzyme aggregates; Groove; Microchannel; Microreactor; Urease |
| Substance Nomenclature: | 0 (Enzymes, Immobilized) EC 3.5.1.5 (Urease) 0 (Cross-Linking Reagents) 0 (Protein Aggregates) |
| Entry Date(s): | Date Created: 20260225 Date Completed: 20260709 Latest Revision: 20260709 |
| Update Code: | 20260711 |
| DOI: | 10.1016/j.enzmictec.2026.110835 |
| PMID: | 41740220 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-0909 |
|---|---|
| DOI: | 10.1016/j.enzmictec.2026.110835 |