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A large-scale optogenetic neurophysiology platform for improving accessibility in non-human primate behavioral experiments.

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Τίτλος: A large-scale optogenetic neurophysiology platform for improving accessibility in non-human primate behavioral experiments.
Συγγραφείς: Griggs DJ; University of Washington Department of Electrical and Computer Engineering, Seattle, USA.; Washington National Primate Research Center, Seattle, USA., Stanis N; Washington National Primate Research Center, Seattle, USA.; University of Washington Department of Bioengineering, Seattle, USA., Bloch J; Washington National Primate Research Center, Seattle, USA.; University of Washington Department of Bioengineering, Seattle, USA., Zhou J; Washington National Primate Research Center, Seattle, USA.; University of Washington Department of Bioengineering, Seattle, USA., Khateeb K; Washington National Primate Research Center, Seattle, USA.; University of Washington Department of Bioengineering, Seattle, USA., Fisher S; University of Washington Department of Electrical and Computer Engineering, Seattle, USA.; Washington National Primate Research Center, Seattle, USA., Shupe L; University of Washington Department of Neurobiology and Biophysics, Seattle, USA., Schwock F; University of Washington Department of Electrical and Computer Engineering, Seattle, USA.; Washington National Primate Research Center, Seattle, USA., Fetz EE; Washington National Primate Research Center, Seattle, USA.; University of Washington Department of Neurobiology and Biophysics, Seattle, USA., Jahanian H; University of Washington Department of Radiology, Seattle, USA., Yazdan-Shahmorad A; University of Washington Department of Electrical and Computer Engineering, Seattle, USA. azadehy@uw.edu.; Washington National Primate Research Center, Seattle, USA. azadehy@uw.edu.; University of Washington Department of Bioengineering, Seattle, USA. azadehy@uw.edu.
Πηγή: Nature communications [Nat Commun] 2026 Feb 24; Vol. 17 (1). Date of Electronic Publication: 2026 Feb 24.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
Imprint Name(s): Original Publication: [London] : Nature Pub. Group
Ιατρικοί όροι (MeSH): Optogenetics*/methods , Optogenetics*/instrumentation , Behavior, Animal*/physiology , Neurophysiology*/methods , Neurophysiology*/instrumentation, Electrocorticography/methods ; Neurons/physiology ; Animals ; Macaca mulatta ; Male
Περίληψη: Optogenetics has been a powerful scientific tool for two decades, yet its integration with non-human primate (NHP) electrophysiology has been limited due to several technical challenges. These include a lack of electrode arrays capable of supporting large-scale and long-term optical access, inaccessible viral vector delivery methods for transfection of large regions of cortex, a paucity of hardware designed for large-scale patterned cortical illumination, and limited designs for multi-modal experimentation. To address these gaps, we introduce a highly accessible platform integrating optogenetics and electrophysiology for behavioral and neural modulation with neurophysiological recording in NHPs. We employed this platform in two rhesus macaques and showcased its capability of optogenetically disrupting reaches, while simultaneously monitoring ongoing electrocorticography activity underlying the stimulation-induced behavioral changes. The platform exhibits long-term stability and functionality, thereby facilitating large-scale electrophysiology, optical imaging, and optogenetics over months, which is crucial for translationally relevant multi-modal studies of neurological and neuropsychiatric disorders.
(© 2026. The Author(s).)
Competing Interests: Competing interests: The authors declare no competing interests.
Σχόλια: Update of: bioRxiv. 2025 Sep 02:2024.06.25.600719. doi: 10.1101/2024.06.25.600719.. (PMID: 38979206)
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Grant Information: 1T32 MH132518 U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH); P51 OD010425 United States CD ODCDC CDC HHS; R01 NS119395 United States NS NINDS NIH HHS; P51 OD0104 U.S. Department of Health & Human Services | NIH | NIH Office of the Director (OD); F99 NS130828 United States NS NINDS NIH HHS; R01 MH125429 United States MH NIMH NIH HHS; K25 AG086663 United States AG NIA NIH HHS; K01 AG071798 United States AG NIA NIH HHS; Graduate Research Fellowship Program (GRFP) National Science Foundation (NSF)
Entry Date(s): Date Created: 20260224 Date Completed: 20260709 Latest Revision: 20260927
Update Code: 20260927
PubMed Central ID: PMC13043788
DOI: 10.1038/s41467-026-69448-3
PMID: 41735306
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2041-1723
DOI:10.1038/s41467-026-69448-3