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Synthesis and Characterization of Polyethylenimine-Coated Magnetic Nanoparticles for DNA Binding Toward Pollen-Directed Gene Delivery Applications.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Synthesis and Characterization of Polyethylenimine-Coated Magnetic Nanoparticles for DNA Binding Toward Pollen-Directed Gene Delivery Applications.
Συγγραφείς: Esha KJ, Roy A, Shamsudduha M, Rashid TU, Alam MS, Das H, Islam T, Rahman M, Ullah SM, Habib A
Πηγή: IEEE transactions on nanobioscience [IEEE Trans Nanobioscience] 2026 Jul; Vol. 25 (3), pp. 312-320.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Institute of Electrical and Electronics Engineers Country of Publication: United States NLM ID: 101152869 Publication Model: Print Cited Medium: Internet ISSN: 1558-2639 (Electronic) Linking ISSN: 15361241 NLM ISO Abbreviation: IEEE Trans Nanobioscience Subsets: MEDLINE
Imprint Name(s): Original Publication: Piscataway, NJ : Institute of Electrical and Electronics Engineers, c2002-
Ιατρικοί όροι (MeSH): Polyethyleneimine*/chemistry , Magnetite Nanoparticles*/chemistry , Pollen*/chemistry , Pollen*/genetics , Pollen*/metabolism , DNA*/chemistry , DNA*/metabolism , DNA*/genetics , Gene Transfer Techniques*, Magnetic Iron Oxide Nanoparticles/chemistry
Περίληψη: Ensuring global food security remains a pressing challenge due to the growing world population and accelerating climate change. Genetic modification provides a pathway to developing crops with improved traits, yet conventional approaches rely heavily on tissue culture regeneration, which is complex, time-consuming, and often impractical for many crop species. Recently, pollen magnetofection has emerged as a tissue culture-independent alternative for plant genetic transformation. In this study, we investigated polyethylenimine (PEI)-coated Fe3O4 (Fe3O4@PEI) magnetic nanoparticles (MNPs) as potential carriers for pollen-mediated gene delivery, focusing on their synthesis, characterization, and DNA-binding capacity. The Fe3O4@PEI MNPs were synthesized via a one-step in situ coating method and their properties were compared with bare Fe3O4 MNPs synthesized separately. The nanostructures were systematically characterized in terms of size, morphology, crystal structure, surface functionalization, elemental composition, and magnetic behavior, confirming successful in situ PEI coating without post-synthesis modification. Compared to bare Fe3O4 MNPs, Fe3O4@PEI MNPs displayed reduced agglomeration, a more uniform size distribution, and improved colloidal stability. The nanoparticles had an average particle size of approximately 30 nm and an enhanced DNA-binding performance: up to 6 ng of linear DNA per $\mu $ g of MNPs (DNA/MNP mass ratio of 0.006:1) and complete retention of plasmid DNA across the tested range. These results support the feasibility of Fe3O4@PEI nanocarriers for pollen-directed gene delivery in plants.
Substance Nomenclature: 9002-98-6 (Polyethyleneimine)
0 (Magnetite Nanoparticles)
9007-49-2 (DNA)
Entry Date(s): Date Created: 20260223 Date Completed: 20260702 Latest Revision: 20260702
Update Code: 20260703
DOI: 10.1109/TNB.2026.3666861
PMID: 41729654
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1558-2639
DOI:10.1109/TNB.2026.3666861