Academic Journal
A novel sterilization mechanism of cold plasma coupled with low-dose ZnO nanoparticles for food Decontamination: Synergistic reactive species generation and zinc ion release.
| Τίτλος: | A novel sterilization mechanism of cold plasma coupled with low-dose ZnO nanoparticles for food Decontamination: Synergistic reactive species generation and zinc ion release. |
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| Συγγραφείς: | Xu HB; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Henan Key Laboratory of Ion-beam Bioengineering, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Sanya Institute, Zhengzhou University, Zhengzhou 450001, China., Du MR; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China; Henan Key Laboratory of Ion-beam Bioengineering, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China., Jiao Z; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Henan Key Laboratory of Ion-beam Bioengineering, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Sanya Institute, Zhengzhou University, Zhengzhou 450001, China., Guan PF; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Henan Key Laboratory of Ion-beam Bioengineering, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Sanya Institute, Zhengzhou University, Zhengzhou 450001, China. Electronic address: guanpanfeng@zzu.edu.cn., Ma RN; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Henan Key Laboratory of Ion-beam Bioengineering, School of Agriculture and Biomanufacturing, Zhengzhou University, Zhengzhou, 450052, China; Sanya Institute, Zhengzhou University, Zhengzhou 450001, China. Electronic address: maruonan@zzu.edu.cn. |
| Πηγή: | Food microbiology [Food Microbiol] 2026 Aug; Vol. 137, pp. 105032. Date of Electronic Publication: 2025 Dec 27. |
| Τύπος έκδοσης: | Journal Article; Evaluation Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: England NLM ID: 8601127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9998 (Electronic) Linking ISSN: 07400020 NLM ISO Abbreviation: Food Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: London : Elsevier Original Publication: London ; Orlando : Academic Press, c1984- |
| Ιατρικοί όροι (MeSH): | Zinc Oxide*/pharmacology , Zinc Oxide*/chemistry , Plasma Gases*/pharmacology , Plasma Gases*/chemistry , Decontamination*/methods , Decontamination*/instrumentation , Sterilization*/methods , Zinc*/chemistry , Zinc*/metabolism , Zinc*/pharmacology , Metal Nanoparticles*/chemistry, Reactive Oxygen Species/metabolism ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/growth & development ; Nanoparticles/chemistry ; Food Microbiology |
| Περίληψη: | Zinc oxide nanoparticles (ZnO NPs) have emerged as novel eco-friendly method for food sterilization. However, the usage of high-concentration ZnO NPs exhibit biological toxicity. To overcome the challenge, this study proposed the dual sterilization strategy coupling cold atmospheric plasma (CAP) with hydrophilic ZnO (H-ZnO) NPs for food decontamination to reduce ZnO NPs potential risk and investigated the synergistic sterilization effects and mechanism of CAP coupled with H-ZnO NPs at different concentration (0.001, 0.01, and 0.1 g/L). Results show that the combined treatment can efficiently inactivate Gram-negative (E. coli and S. enterica) and Gram-positive bacteria (L.monocytogenes and S. aureus) on the surface of blueberries. CAP +0.01 g/L H-ZnO NPs had the most obvious synergistic sterilization, and the bacterial reduction was increased by 0.5 log at least among the four strains. The combined treatment of CAP and 0.01 g/L H-ZnO NPs led to the enhanced generation of reactive oxygen and nitrogen species (RONS) and Zn2+ release from H-ZnO NPs in the solution. Meanwhile, the released Zn2+ flowed into the cell and accordingly increased the intracellular ROS level (670 %), consequently resulting in the improved bacterial inactivation. The low concentration of H-ZnO NPs (0.001 g/L) could not effectively cause membrane potential depolarization, and thus leading to a poor result of coupled sterilization. The high concentration of H-ZnO NPs (0.1 g/L) attaching on S. aureus cell surface may obstruct the interaction between CAP and S. aureus, which showed the worst synergistic bactericidal efficiency. This study proposed CAP/ZnO synergy offers a scalable, low-chemical alternative to conventional decontamination methods, which will address the emerging challenges in food management. (Copyright © 2025 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors have no conflict of interest to declare. |
| Contributed Indexing: | Keywords: Cold atmospheric plasma; Food decontamination; Hydrophilic ZnO nanoparticles; Synergistic effect |
| Substance Nomenclature: | SOI2LOH54Z (Zinc Oxide) 0 (Plasma Gases) 0 (Reactive Oxygen Species) J41CSQ7QDS (Zinc) |
| Entry Date(s): | Date Created: 20260221 Date Completed: 20260708 Latest Revision: 20260708 |
| Update Code: | 20260708 |
| DOI: | 10.1016/j.fm.2025.105032 |
| PMID: | 41722977 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1095-9998 |
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| DOI: | 10.1016/j.fm.2025.105032 |