Academic Journal
HipSTR-UI: A cross-platform graphical interface for accessible str genotyping from next-generation sequencing data.
| Τίτλος: | HipSTR-UI: A cross-platform graphical interface for accessible str genotyping from next-generation sequencing data. |
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| Συγγραφείς: | Frontanilla TS; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14049-900, Brasil. Electronic address: tfronta@usp.br., de Sousa Ferrari M; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14049-900, Brasil., Ayala J; Facultad de Ingeniería Informática, Universidad de la Integración de las Américas, Asunción 00120-6, Paraguay., Mendes-Junior CT; Departamento de Química, Laboratório de Pesquisas Forenses e Genômicas, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14040-901, Brasil. |
| Πηγή: | Forensic science international. Genetics [Forensic Sci Int Genet] 2026 Jun; Vol. 84, pp. 103456. Date of Electronic Publication: 2026 Feb 19. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101317016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0326 (Electronic) Linking ISSN: 18724973 NLM ISO Abbreviation: Forensic Sci Int Genet Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam : Elsevier |
| Ιατρικοί όροι (MeSH): | High-Throughput Nucleotide Sequencing* , Microsatellite Repeats* , Software* , User-Computer Interface* , Computer Graphics* , Genotyping Techniques*, Humans ; Genotype ; Sequence Analysis, DNA |
| Περίληψη: | Next-generation sequencing (NGS) has expanded the scope of forensic genetics by providing sequence-level resolution of Short Tandem Repeats (STRs). We developed HipSTR-UI, a cross-platform graphical interface that integrates precompiled HipSTR binaries for Windows, macOS, and Linux. The interface automates the genotyping workflow, from alignment files (BAM/CRAM) and target regions with the reference genome (FASTA) to the generation of genotypes in VCF format. HipSTR-UI includes a graphical parameter panel and exportable results. The validation was performed using Phase 3 of the 1000 Genomes Project, previously analyzed with the HipSTR command-line version. HipSTR-UI accurately reproduced the results of HipSTR, achieving 100 % concordance in genotype calls. As expected, no discrepancies were observed in concordance rates, discordant loci, or quality scores, since the interface executes the same commands as the original HipSTR tool. HipSTR-UI combines the robustness of HipSTR with a user-friendly and multilingual interface, bridging the gap between advanced sequencing technologies and routine forensic applications. Additionally, it facilitates adoption in routine forensic workflows, including human identification and kinship analysis. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper. |
| Contributed Indexing: | Keywords: Bioinformatics tools; HipSTR; Next-generation sequencing; STR genotyping |
| Entry Date(s): | Date Created: 20260221 Date Completed: 20260708 Latest Revision: 20260708 |
| Update Code: | 20260708 |
| DOI: | 10.1016/j.fsigen.2026.103456 |
| PMID: | 41722243 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1878-0326 |
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| DOI: | 10.1016/j.fsigen.2026.103456 |