Academic Journal
Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers.
| Τίτλος: | Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers. |
|---|---|
| Συγγραφείς: | Bharti; Council of Scientific and Industrial Research (CSIR), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160036, India.; Department of Biotechnology, Panjab University, Chandigarh 160014, India., Bhardwaj P; Council of Scientific and Industrial Research (CSIR), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160036, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India., Bisht B; Council of Scientific and Industrial Research (CSIR), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160036, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India., Bala R; Council of Scientific and Industrial Research (CSIR), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160036, India., Sagrika; Council of Scientific and Industrial Research (CSIR), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160036, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India., Rohit M; Department of Cardiology, PGIMER, Chandigarh 160012, India., Singh J; Department of Biotechnology, Panjab University, Chandigarh 160014, India., Bhalla V; Council of Scientific and Industrial Research (CSIR), Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160036, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. |
| Πηγή: | ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2026 Mar 04; Vol. 18 (8), pp. 12423-12431. Date of Electronic Publication: 2026 Feb 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, D.C. : American Chemical Society |
| Ιατρικοί όροι (MeSH): | Colorimetry*/methods , Troponin I*/blood , Troponin I*/analysis , Metal Nanoparticles*/chemistry , Myocardial Infarction*/diagnosis, Biomarkers/blood ; Biomarkers/analysis ; Silver/chemistry ; Aptamers, Nucleotide/chemistry ; Biosensing Techniques/methods ; Urease/chemistry ; Urease/metabolism ; Humans ; Limit of Detection |
| Περίληψη: | Cardiac troponin I (cTnI) is a key biomarker for acute myocardial infarction (AMI); however, the available detection methods are limited by slow responses, complex steps, and costly instrumentation, restricting their suitability for rapid or point-of-care testing. Herein, we report a rapid and sensitive colorimetric assay for the detection of cTnI based on a nanoparticle shielding layer and its interfacial interactions with an enzyme. To achieve high sensitivity, we employed a dual-affinity silver nanoparticle (AgNP) probe coated with two highly specific affinity molecules, i.e., aptamer (Apt) and antibody (Ab), offering effective detection of the target molecule. The affinity layer coating was inherently imperfect, leaving exposed regions at the interface of the AgNP probe to inhibit the enzyme (urease) by Ag-SH interaction. Upon introduction of cTnI, the probe selectively binds to it, and a target-induced shielding layer is formed that prevents the interaction of the enzyme with the AgNP surface, rendering the enzyme active in solution. Consequently, the active enzyme generates a colored endpoint for visual detection in <15 min with a detection limit of 0.088 ng/mL. We assessed the assay performance in a complex matrix to evaluate the stringency. The working of the assay was studied using microscale thermophoresis (MST), which showed complete masking of the urease-particle interaction in the presence of cTnI. Unlike surface-based methods, such as ELISA, our solution-phase assay eliminates immobilization and blocking steps and achieves enhanced sensitivity, representing a paradigm shift in biomarker detection. |
| Contributed Indexing: | Keywords: cardiac troponin I (cTnI); colorimetric assay; microscale thermophoresis (MST); nanoparticle shielding layer; rapid detection |
| Substance Nomenclature: | 0 (Troponin I) 0 (Biomarkers) 3M4G523W1G (Silver) 0 (Aptamers, Nucleotide) EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 20260219 Date Completed: 20260708 Latest Revision: 20260708 |
| Update Code: | 20260708 |
| DOI: | 10.1021/acsami.5c21395 |
| PMID: | 41709411 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1944-8252 |
|---|---|
| DOI: | 10.1021/acsami.5c21395 |