Academic Journal
L‒asparaginase activity in some endophytic fungi: Glutaminase‒free and low urease co‒activities.
| Τίτλος: | L‒asparaginase activity in some endophytic fungi: Glutaminase‒free and low urease co‒activities. |
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| Συγγραφείς: | Zaeimian Z; Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran., Fotouhifar KB; Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran., Farzaneh M; Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran. |
| Πηγή: | PloS one [PLoS One] 2026 Feb 13; Vol. 21 (2), pp. e0339829. Date of Electronic Publication: 2026 Feb 13 (Print Publication: 2026). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: San Francisco, CA : Public Library of Science |
| Ιατρικοί όροι (MeSH): | Asparaginase*/metabolism , Urease*/metabolism , Glutaminase*/metabolism , Endophytes*/enzymology , Endophytes*/isolation & purification , Cladosporium*/enzymology , Fungi*/enzymology, Alternaria/enzymology ; Asparagine/metabolism ; Asparagine/pharmacology ; Culture Media |
| Περίληψη: | In this study, L‒asparaginase production in several endophytic fungi was evaluated along with their L‒glutaminase and urease co-activities. The effect of L‒asparagine and different culture media on L-asparaginase production were also evaluated. Among the 62 investigated isolates, 49 isolates exhibited L‒asparaginase activity, and the maximum zone index (6.58) was observed in Cladosporium perangustum EL1. Evaluation of L‒glutaminase and urease co-activities in L‒asparaginase-positive isolates screened 19 isolates with no L‒glutaminase activity and four isolates with minimum urease production. L‒asparaginase activity was quantified in 12 selected isolates using the Nesslerization method. Cladosporium cladosporioides Kr5-2 exhibited the maximum L‒asparaginase activity (10.78 U mL-1). Alternaria brassicae C showed high L‒asparaginase activity (7.07 U mL-1) free of L‒glutaminase, and low urease co-activity (1.97 U mL-1). Assessment of the effect of L‒asparagine on L‒asparaginase activity showed that the enzyme is inducible and substrate-regulated. Evaluation of ten different culture media showed that all isolates were able to produce L‒asparaginase on Mineral salts agar and Citrate agar culture media. Also, Cerelose ammonium nitrate agar, Kuehner basal culture medium, and Piefer, Humphrey, and Acree culture medium inhibited L‒asparaginase production in the majority of the isolates. This is the first report of L‒asparaginase production by endophytic fungi isolated from Taxus baccata, Pistacia vera, Prunus avium, Prunus cerasus, and Punica granatum, as well as the investigation of their L‒glutaminase and urease co-activities. Among the evaluated culture media, Mineral salts agar and Citrate agar culture media are suggested here as alternate for MCD. Moreover, Alternaria brassicae C is recommended as a promising isolate for future commercial L‒asparaginase production. (Copyright: © 2026 Zaeimian et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.) |
| Competing Interests: | The authors have declared that no competing interests exist. |
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| Substance Nomenclature: | EC 3.5.1.1 (Asparaginase) EC 3.5.1.5 (Urease) EC 3.5.1.2 (Glutaminase) 0 (Culture Media) 7006-34-0 (Asparagine) |
| Entry Date(s): | Date Created: 20260213 Date Completed: 20260707 Latest Revision: 20260707 |
| Update Code: | 20260708 |
| PubMed Central ID: | PMC12904411 |
| DOI: | 10.1371/journal.pone.0339829 |
| PMID: | 41686855 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1932-6203 |
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| DOI: | 10.1371/journal.pone.0339829 |