Academic Journal
Neuroprotective effects of pepsin-hydrolyzed ovotransferrin on H2O2-induced oxidative damage in SH-SY5Y neuronal cells.
| Τίτλος: | Neuroprotective effects of pepsin-hydrolyzed ovotransferrin on H |
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| Συγγραφείς: | Kim HJ; Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea., Yu SW; Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea., Kim KT; Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea; Research Institute, WithBio Inc., Seoul 05029, Republic of Korea., Ahn DU; Department of Animal Science, Iowa State University, Ames, IA, 50011, USA., Lee NK; Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea; Research Institute, WithBio Inc., Seoul 05029, Republic of Korea., Paik HD; Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea; Research Institute, WithBio Inc., Seoul 05029, Republic of Korea. Electronic address: hdpaik@konkuk.ac.kr. |
| Πηγή: | Bioorganic chemistry [Bioorg Chem] 2026 Jun 05; Vol. 173, pp. 109613. Date of Electronic Publication: 2026 Feb 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: United States NLM ID: 1303703 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2120 (Electronic) Linking ISSN: 00452068 NLM ISO Abbreviation: Bioorg Chem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: New York, London, Academic Press. |
| Ιατρικοί όροι (MeSH): | Neuroprotective Agents*/pharmacology , Neuroprotective Agents*/chemistry , Oxidative Stress*/drug effects , Conalbumin*/pharmacology , Conalbumin*/chemistry , Pepsin A*/metabolism , Pepsin A*/chemistry , Hydrogen Peroxide*/pharmacology , Hydrogen Peroxide*/antagonists & inhibitors , Neurons*/drug effects , Neurons*/metabolism , Antioxidants*/pharmacology , Antioxidants*/chemistry, Cell Survival/drug effects ; Apoptosis/drug effects ; Reactive Oxygen Species/metabolism ; Humans ; Hydrolysis ; Dose-Response Relationship, Drug ; Cell Line, Tumor ; Structure-Activity Relationship |
| Περίληψη: | Neurodegenerative diseases are considered critical disorders due to the absence of effective treatments and serious damage to brain function. Ovotransferrin, an egg white protein, and its hydrolysate obtained using proteolytic enzymes have been reported to have beneficial effects on various bioactive functions. Therefore, the purpose of this study was to investigate the neuroprotective effects of enzymatically hydrolyzed ovotransferrin in SH-SY5Y cells exposed to oxidative stress. Hydrolyzed ovotransferrin showed higher antioxidative activities than pure ovotransferrin. Furthermore, 0.5 mg/mL of pepsin-derived ovotransferrin hydrolysate (OHPE) exhibited the highest cell viability (81.89%), and OHPE effectively reduced cellular ROS accumulation (72%) and decreased total apoptosis rate (46.41%). These anti-apoptotic and antioxidative effects were influenced by the regulation of the mRNA and protein expression of Bax, Bcl-2, caspase-9, caspase-3, Nrf2, and HO-1. Additionally, nine peptides comprising OHPE were identified by LC-MS/MS and compared with the sequences reported in DFBP to explore their potential as antioxidants. These results indicate that OHPE can be used as a nature-derived compound to prevent neurodegenerative diseases by inhibiting ROS interactions and regulating protein expression in neuronal cells. (Copyright © 2024. Published by Elsevier Inc.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Enzyme hydrolysis; Hydrogen peroxide; Neuroprotective effect; Ovotransferrin; Pepsin |
| Substance Nomenclature: | 0 (Neuroprotective Agents) 1391-06-6 (Conalbumin) EC 3.4.23.1 (Pepsin A) BBX060AN9V (Hydrogen Peroxide) 0 (Reactive Oxygen Species) 0 (Antioxidants) |
| Entry Date(s): | Date Created: 20260212 Date Completed: 20260707 Latest Revision: 20260707 |
| Update Code: | 20260708 |
| DOI: | 10.1016/j.bioorg.2026.109613 |
| PMID: | 41679204 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1090-2120 |
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| DOI: | 10.1016/j.bioorg.2026.109613 |