Academic Journal
Temperature-responsive nanozyme-mediated enrichment-confinement dual strategy breaks the non-responsive barrier for Triphenols: Highly responsive detection and differentiation of tea.
| Τίτλος: | Temperature-responsive nanozyme-mediated enrichment-confinement dual strategy breaks the non-responsive barrier for Triphenols: Highly responsive detection and differentiation of tea. |
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| Συγγραφείς: | Li J; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China., Han Q; Jilin Provincial Key Laboratory of Water Resources and Water Environment, College of New Energy and Environment, Jilin University, Changchun, 130021, PR China., Zheng X; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China., Zhen L; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China., Li Y; Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, College of New Energy and Environment, Jilin University, Changchun, 130021, PR China; Jilin Provincial Key Laboratory of Water Resources and Water Environment, College of New Energy and Environment, Jilin University, Changchun, 130021, PR China., Huang H; College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China. Electronic address: huanghui@jlu.edu.cn. |
| Πηγή: | Biosensors & bioelectronics [Biosens Bioelectron] 2026 Jun 01; Vol. 301, pp. 118503. Date of Electronic Publication: 2026 Feb 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| Imprint Name(s): | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| Ιατρικοί όροι (MeSH): | Tea*/chemistry , Catechol Oxidase*/chemistry , Biosensing Techniques*/methods , Phenols*/analysis , Phenols*/chemistry , Polyphenols*/analysis , Polyphenols*/chemistry , Nanostructures*/chemistry, Temperature ; Hydrophobic and Hydrophilic Interactions ; Limit of Detection |
| Περίληψη: | The predominant ortho-trihydroxyphenyl structure in tea polyphenols requires highly specific sensing materials for their detection. However, both natural enzymes and current polyphenol oxidase-like nanozymes exhibit inadequate responsiveness to this specific configuration. To overcome this limitation, a dual "enrichment-confinement" strategy was proposed, effectively addressing the suboptimal detection performance for triphenolic compounds. The efficacy of this strategy relies on a switchable hydrophilic-to-hydrophobic transition: substrate enrichment is driven by extended hydrophilic chains at low temperatures, whereas heating induces hydrophobic collapse and nanoscale contraction, thereby activating spatial confinement. This conformational change not only shortens the interactive distance between the nanozyme and the substrate but also mitigates undesirable substrate aggregation. Theoretical calculations further reveal that the interactions between the nanozyme and the thermosensitive polymer are primarily governed by van der Waals forces and hydrogen bonding, which notably preserve the nanozyme intrinsic catalytic activity. This method enables the precise differentiation of tea polyphenols with a detection limit as low as 100 nM. Notably, this technology extends beyond the detection of tea triphenols, broadening the application scope of polyphenol oxidase-like nanozymes and providing a novel paradigm for the sensitive detection of diverse triphenolic compounds. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Hydrophilicity regulation; Mobile APP; Polyphenol oxidase-like activity; Temperature-responsive nanozyme; Triphenolic structure detection |
| Substance Nomenclature: | 0 (Tea) EC 1.10.3.1 (Catechol Oxidase) 0 (Phenols) 0 (Polyphenols) |
| Entry Date(s): | Date Created: 20260211 Date Completed: 20260707 Latest Revision: 20260707 |
| Update Code: | 20260707 |
| DOI: | 10.1016/j.bios.2026.118503 |
| PMID: | 41671932 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2026.118503 |