Academic Journal
Se nanoparticles-coated, PLGA-based spheres for biomedical applications: Cytotoxicity, genotoxicity, oxidative stress, biodistribution, and scintigraphic study.
| Τίτλος: | Se nanoparticles-coated, PLGA-based spheres for biomedical applications: Cytotoxicity, genotoxicity, oxidative stress, biodistribution, and scintigraphic study. |
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| Συγγραφείς: | Stevanović M; Group for Biomedical Engineering and Nanobiotechnology, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Belgrade, Serbia., Nunić J; Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia., Filipič M; Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia., Digilio G; Department of Science and Technologic Innovation, Università Del Piemonte Orientale 'Amedeo Avogadro', Alessandria, Italy., Fragogeorgi E; National Center for Scientific Research (NCSR) 'Demokritos', Institute of Nuclear & Radiological Sciences & Technology, Energy &Safety, Athens, Greece., Loudos G; BIOEMTECH, Gerakas, Greece. |
| Πηγή: | Journal of biomaterials applications [J Biomater Appl] 2026 Sep; Vol. 41 (3), pp. 431-446. Date of Electronic Publication: 2026 Feb 09. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Sage Publications Country of Publication: England NLM ID: 8813912 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-8022 (Electronic) Linking ISSN: 08853282 NLM ISO Abbreviation: J Biomater Appl Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2002- : London : Sage Publications Original Publication: Lancaster, PA : Technomic Pub. Co., Inc., [c1986- |
| Ιατρικοί όροι (MeSH): | Polyglycolic Acid*/chemistry , Polyglycolic Acid*/pharmacokinetics , Lactic Acid*/chemistry , Lactic Acid*/pharmacokinetics , Nanoparticles*/chemistry , Nanoparticles*/toxicity , Selenium*/chemistry , Selenium*/pharmacokinetics , Selenium*/toxicity, Oxidative Stress/drug effects ; Cell Survival/drug effects ; Reactive Oxygen Species/metabolism ; Humans ; Polylactic Acid-Polyglycolic Acid Copolymer ; Animals ; Hep G2 Cells ; Tissue Distribution ; Mice ; DNA Damage |
| Περίληψη: | Conventional approaches to prevent and treat diseases, particularly liver disorders, often fall short, highlighting the urgent need for innovative strategies and materials in RNA therapeutics and genetic drug delivery. This study investigates the synthesis, characterization, and biological evaluation of poly (DL-lactide-co-glycolide) (PLGA) spherical particles as a novel drug delivery system for selenium nanoparticles (SeNp), presenting a promising (PLGA/SeNp) platform for enhancing the efficacy of genetic therapies aimed at liver diseases. We assessed the effects of PLGA/SeNp nanoparticles in vitro using human hepatoma cell lines (HepG2 cells), focusing on (i) cell viability, (ii) intracellular reactive oxygen species (ROS) generation, and (iii) genotoxic response. The findings indicated that PLGA/SeNp nanoparticles maintained cell viability, exhibited minimal ROS generation, and demonstrated low genotoxicity, underscoring their biocompatibility for therapeutic applications. Furthermore, this study explored the in vivo biodistribution and pharmacokinetics of PLGA and PLGA/SeNp particles through non-invasive dynamic imaging techniques. By radiolabeling with technetium-99m (Tc99m), we conducted scintigraphic imaging to analyze biodistribution. Our in vivo results revealed significant differences in the biodistribution profiles of PLGA and PLGA/SeNp formulations at 24 h post-injection, with PLGA/SeNp showing enhanced hepatic, splenic, and pulmonary uptake compared to PLGA. These findings emphasize the unique pharmacokinetic properties of the PLGA/SeNp system, presenting a viable option for RNA-based therapeutics in liver disease management. |
| Competing Interests: | Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. |
| Contributed Indexing: | Keywords: PLGA; biomedical applications; nanotechnology; selenium nanoparticles |
| Substance Nomenclature: | 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer) 26009-03-0 (Polyglycolic Acid) 33X04XA5AT (Lactic Acid) H6241UJ22B (Selenium) 0 (Reactive Oxygen Species) |
| Entry Date(s): | Date Created: 20260209 Date Completed: 20260825 Latest Revision: 20260825 |
| Update Code: | 20260825 |
| DOI: | 10.1177/08853282261422858 |
| PMID: | 41661698 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1530-8022 |
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| DOI: | 10.1177/08853282261422858 |