Academic Journal
SIRT3 ameliorates hepatic inflammation, oxidative stress, and fibrosis in HFD- or MCD diet-fed mice.
| Τίτλος: | SIRT3 ameliorates hepatic inflammation, oxidative stress, and fibrosis in HFD- or MCD diet-fed mice. |
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| Συγγραφείς: | Lv H; Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China., Sun W; Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China., Tian C; Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China., Bian C; Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China., Lu Z; Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China., Guo X; Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Electronic address: xguo@sdu.edu.cn. |
| Πηγή: | The Journal of nutritional biochemistry [J Nutr Biochem] 2026 Jun; Vol. 152, pp. 110276. Date of Electronic Publication: 2026 Jan 29. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 9010081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4847 (Electronic) Linking ISSN: 09552863 NLM ISO Abbreviation: J Nutr Biochem Subsets: MEDLINE |
| Imprint Name(s): | Publication: <1996->: New York, NY : Elsevier Science Original Publication: Stoneham, MA, USA : Butterworths, c1990- |
| Ιατρικοί όροι (MeSH): | Sirtuin 3*/metabolism , Sirtuin 3*/genetics , Diet, High-Fat*/adverse effects , Liver Cirrhosis*/metabolism , Oxidative Stress*, Liver/metabolism ; Liver/pathology ; Inflammation/metabolism ; Animals ; Male ; Mice, Inbred C57BL ; Mice |
| Περίληψη: | Metabolic dysfunction-associated steatotic liver disease and metabolic dysfunction-associated steatohepatitis (MASLD/MASH), has emerged as one of the leading causes of cirrhosis and hepatocellular carcinoma. Currently, no specific pharmacological treatment is available for MASLD/MASH patients. Sirtuin 3 (SIRT3) plays a pivotal role in regulating mitochondrial metabolism, antioxidant defenses, and the maintenance of cellular homeostasis. To investigate the effects of SIRT3 overexpression on inflammation, oxidative stress, and fibrosis in mice. Mice were injected an SIRT3 overexpressed adeno-associated virus (AAV-SIRT3) vector or a control vector (AAV-GFP) by intravenous tail vein injection. Six-week-old mice were randomly assigned to four groups: CD-GFP, CD-SIRT3, HFD-GFP, and HFD-SIRT3. Mice received tail vein injections according to the group assignment, followed by a 12-week feeding regimen with either a chow diet (CD) or a high-fat diet (HFD). Eleven-week-old mice were randomly divided into four groups: MCS-GFP, MCS-SIRT3, MCD-GFP, and MCD-SIRT3 groups. Tail vein injections were administered prior to a 5-week dietary intervention using either a methionine-choline-sufficient diet (MCS) or a methionine-choline-deficient diet (MCD). In both HFD-and MCD diet-fed mice, SIRT3 overexpression improved serum ALT and AST levels, decreased hepatic TG levels, reduced macrophage infiltration, and decreased inflammatory signals and cytokines. In addition, hepatic SIRT3 overexpression reduced ROS production and increased antioxidative protein levels, such as HO-1, NQO1, and NRF2, as well as increased Mitochondrial SOD2 deacetylation. Furthermore, SIRT3 overexpression decreased the levels of fibrogenic genes including Col1α1, Col3α1, and α-SMA. SIRT3 overexpression significantly reduced hepatic lipid accumulation, inflammation, oxidative stress, and fibrosis in HFD-and MCD diet-fed mice. (Copyright © 2026 Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that there are no conflicts of interest. |
| Contributed Indexing: | Keywords: Fibrosis; Inflammation; MASLD/MASH; ROS; SIRT3 |
| Substance Nomenclature: | EC 3.5.1.- (Sirtuin 3) 0 (Sirt3 protein, mouse) |
| Entry Date(s): | Date Created: 20260131 Date Completed: 20260706 Latest Revision: 20260706 |
| Update Code: | 20260707 |
| DOI: | 10.1016/j.jnutbio.2026.110276 |
| PMID: | 41619818 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-4847 |
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| DOI: | 10.1016/j.jnutbio.2026.110276 |