Influences of oils on the persistence and recovery of DNA.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Influences of oils on the persistence and recovery of DNA.
Συγγραφείς:	Kaesler T; College of Science & Engineering, Flinders University, Adelaide, South Australia, Australia., Linacre A; College of Science & Engineering, Flinders University, Adelaide, South Australia, Australia., Kirkbride KP; College of Science & Engineering, Flinders University, Adelaide, South Australia, Australia.
Πηγή:	Journal of forensic sciences [J Forensic Sci] 2026 Mar; Vol. 71 (2), pp. 938-948. Date of Electronic Publication: 2026 Jan 11.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Blackwell Pub Country of Publication: United States NLM ID: 0375370 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1556-4029 (Electronic) Linking ISSN: 00221198 NLM ISO Abbreviation: J Forensic Sci Subsets: MEDLINE
Imprint Name(s):	Publication: 2006- : Malden, MA : Blackwell Pub. Original Publication: [Chicago, Ill.] : Callaghan and Co., 1956-
Ιατρικοί όροι (MeSH):	DNA/isolation & purification , Mineral Oil/chemistry , DNA Fingerprinting* , Touch* , Specimen Handling*, Humans ; Microsatellite Repeats ; Viscosity ; Polymerase Chain Reaction
Περίληψη:	Trace DNA can be deposited onto a wide range of surface types which can include substrates with a coating of oil: examples being firearm components, power tools, and kitchen utensils. We report on the impacts of oil on DNA recovery and downstream DNA processing. Thumbprints were made on 60 separate glass slides, then stained with Diamond Dye and fluorescent cellular material counted as an estimation of the cells deposited. Aliquots of five different mineral oils, chosen due to their common uses and variation in viscosity, were spread across the entire deposited thumbprint. The thumbprints coated with one of the five oils were left at room temperature for either 1 day or 7 days. A swab was used to collect cellular material which was then processed manually through a DNA extraction process, quantified and STR alleles amplified using the VeriFiler™ Plus STR kit. It was observed that all five oils impacted the initial DNA recovery process by saturating the swab. The presence of oils also impacted the DNA extraction process by interacting with the magnetic resin. Oils with higher viscosity accentuated these observed effects during the DNA extraction process, as the presence of a white precipitate was seen to be carried over into the final eluate. A total of 56% of the 1-day samples and 72% of the 7-day samples resulted in DNA profiles comprised of 12 or more alleles. The results of this study highlighted the possible impacts of collecting samples with oils present on the surface. (© 2026 American Academy of Forensic Sciences.)
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Grant Information:	Attorney-General's Department, Australian Government; Ross Vining Memorial Research Fund; College of Science and Engineering Research Award (CERSA)
Contributed Indexing:	Keywords: DNA IQ; Diamond Dye; PCR inhibition; STR DNA profile; oils; touch DNA
Substance Nomenclature:	9007-49-2 (DNA) 8020-83-5 (Mineral Oil)
Entry Date(s):	Date Created: 20260112 Date Completed: 20260626 Latest Revision: 20260626
Update Code:	20260626
DOI:	10.1111/1556-4029.70249
PMID:	41521515
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1556-4029
DOI:	10.1111/1556-4029.70249