Academic Journal
Προβολή σε EDS

Genetic Characterization of Hepatitis C Virus Among People Who Use Crack Cocaine: A Study Conducted on the Brazilian Amazon Coast.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Genetic Characterization of Hepatitis C Virus Among People Who Use Crack Cocaine: A Study Conducted on the Brazilian Amazon Coast.
Συγγραφείς: Heymbeeck JAA; Residência Multiprofissional de Saúde da Mulher e da Criança, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Grupo de Estudo e Pesquisa em Populações Vulneráveis, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil., do Nascimento WL; Residência Multiprofissional de Saúde da Mulher e da Criança, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Grupo de Estudo e Pesquisa em Populações Vulneráveis, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil., Freitas MCS; Grupo de Estudo e Pesquisa em Populações Vulneráveis, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Programa de Pós-Graduação em Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil., de Sousa Rocha L; Grupo de Estudo e Pesquisa em Populações Vulneráveis, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Programa de Pós-Graduação em Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil., Costa FF; Grupo de Estudo e Pesquisa em Populações Vulneráveis, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Programa de Pós-Graduação em Biologia Ambiental, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil., de Queiroz JPQ; Grupo de Estudo e Pesquisa em Populações Vulneráveis, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Programa de Pós-Graduação em Biologia Ambiental, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil., Simeone D; Afya Faculdade de Ciências Médicas, Bragança 68600-000, PA, Brazil., Martins LC; Residência Multiprofissional de Saúde da Mulher e da Criança, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Programa de Pós-Graduação em Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil.; Núcleo de Medicina Tropical, Universidade Federal do Pará, Belém 66055-240, PA, Brazil., Machado LFA; Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, PA, Brazil., Fischer B; Faculty of Health Sciences, Simon Fraser University, Vancouver, BC V6B 5K3, Canada.; Waypoint Research Institute, Waypoint Centre for Mental Health Care, Penetanguishene, ON L9M 1G3, Canada.; Research & Graduate Studies, University of the Fraser Valley, Abbotsford, BC V2S 7M8, Canada.; Department of Psychiatry, University of Toronto, Toronto, ON M5T 1R8, Canada.; School of Population Health, Faculty of Medical & Health Sciences, Grafton Campus, University of Auckland, Auckland 1023, New Zealand.; Department of Psychiatry, Federal University of São Paulo, São Paulo 04017-030, SP, Brazil., Kupek E; Departamento de Saúde Pública, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil., Oliveira-Filho AB; Residência Multiprofissional de Saúde da Mulher e da Criança, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Grupo de Estudo e Pesquisa em Populações Vulneráveis, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.; Programa de Pós-Graduação em Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil.; Programa de Pós-Graduação em Biologia Ambiental, Universidade Federal do Pará, Bragança 68600-000, PA, Brazil.
Πηγή: Pathogens (Basel, Switzerland) [Pathogens] 2025 Dec 17; Vol. 14 (12). Date of Electronic Publication: 2025 Dec 17.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596317 Publication Model: Electronic Cited Medium: Internet ISSN: 2076-0817 (Electronic) Linking ISSN: 20760817 NLM ISO Abbreviation: Pathogens Subsets: MEDLINE
Imprint Name(s): Original Publication: Basel, Switzerland : MDPI AG, 2012-
Ιατρικοί όροι (MeSH): Crack Cocaine*/adverse effects , Hepacivirus*/genetics , Hepacivirus*/drug effects , Hepacivirus*/classification , Hepatitis C*/epidemiology , Hepatitis C*/virology , Hepatitis C*/drug therapy , Cocaine-Related Disorders*/virology, Brazil/epidemiology ; Drug Resistance, Viral/genetics ; Antiviral Agents/therapeutic use ; Antiviral Agents/pharmacology ; RNA, Viral/genetics ; Humans ; Male ; Adult ; Female ; Genotype ; Middle Aged ; Young Adult ; Phylogeny
Περίληψη: People who use crack cocaine (PWUCC) constitute a key population due to vulnerability and marginalization, especially in a socio-ecologically diverse, relatively isolated region with limited public health infrastructure. This study aimed to perform a genetic characterization of circulating HCV among PWUCC in the municipality of Bragança, situated on the Brazilian Amazon coast, identifying viral genotypes, subtypes, resistance-associated substitutions (RAS)-naturally occurring mutations in the viral genome that can reduce the efficacy of direct-acting antiviral (DAA) agents-and predictions of phenotypic resistance.
Methods: Between 2016 and 2018, biological samples and epidemiological data were obtained from 165 PWUCC. Viral detection was performed using RT-PCR, while genotyping, subtyping, and RAS profiling were conducted through nucleotide sequencing and fragment analysis.
Results: In 165 PWUCC, 22 (13.3%) tested positive for HCV RNA. Most of them had not had access to public health services (91.5%), and more than half (57.0%) reported living in unstable housing conditions. HCV subtypes 1a (27.3%), 1b (40.9%), and 3a (31.8%) were detected. Evidence of resistance associated with DAAs, such as daclatasvir and dasabuvir, was detected in five PWUCC with HCV (22.7%).
Conclusions: The high prevalence of HCV infection, predominantly subtype 1b, and significant levels of resistance are very concerning. This demonstrates the urgent need for targeted public health interventions to expand access to testing, treatment, and effective antiviral therapy in this vulnerable population of the Brazilian Amazon.
References: J Hepatol. 2017 Aug;67(2):224-236. (PMID: 28343981)
Drug Resist Updat. 2018 Mar;37:17-39. (PMID: 29525636)
Bioinformatics. 2014 Nov 15;30(22):3276-8. (PMID: 25095880)
Pathogens. 2019 Nov 14;8(4):. (PMID: 31739623)
Viruses. 2021 May 17;13(5):. (PMID: 34067873)
Infect Genet Evol. 2020 Nov;85:104488. (PMID: 32745809)
Am J Epidemiol. 2002 Apr 1;155(7):645-53. (PMID: 11914192)
Gastroenterology. 2016 Jul;151(1):70-86. (PMID: 27080301)
Arch Virol. 2018 Mar;163(3):617-622. (PMID: 29164400)
Liver Int. 2017 Apr;37(4):514-528. (PMID: 28105744)
PLoS One. 2014 Jan 21;9(1):e86413. (PMID: 24466079)
J Med Virol. 2013 Oct;85(10):1741-5. (PMID: 23852735)
Braz J Med Biol Res. 2005 Jan;38(1):41-9. (PMID: 15665987)
Clin Infect Dis. 2014 Dec 15;59(12):1666-74. (PMID: 25266287)
Cad Saude Publica. 2015 Jul;31(7):1497-504. (PMID: 26248104)
Rev Soc Bras Med Trop. 2008 May-Jun;41(3):238-42. (PMID: 18719801)
PLoS One. 2019 May 7;14(5):e0216327. (PMID: 31063475)
Arch Womens Ment Health. 2010 Jun;13(3):185-91. (PMID: 19760050)
Curr Top Microbiol Immunol. 2013;369:167-98. (PMID: 23463201)
Viruses. 2018 Oct 06;10(10):. (PMID: 30301201)
BMC Infect Dis. 2019 Jul 17;19(1):634. (PMID: 31315569)
Nucleic Acids Res. 2014 Oct;42(18):e144. (PMID: 25120265)
Mem Inst Oswaldo Cruz. 2009 Sep;104(6):892-6. (PMID: 19876562)
J Hepatol. 2023 Jan;78(1):57-66. (PMID: 36031158)
Drug Alcohol Depend. 2007 Jun 15;89(1):1-12. (PMID: 17174481)
Clin Microbiol Infect. 2016 Oct;22(10):833-838. (PMID: 27521803)
Hepatology. 2015 Jan;61(1):56-65. (PMID: 25123381)
J Gastroenterol Hepatol. 2018 Dec;33(12):2029-2036. (PMID: 29864213)
Lancet Gastroenterol Hepatol. 2022 Apr;7(4):277-279. (PMID: 35026143)
Hepatology. 2015 Jan;61(1):77-87. (PMID: 25069599)
Nat Rev Dis Primers. 2017 Mar 02;3:17006. (PMID: 28252637)
Grant Information: MS/SVS-TC 318/2013 Ministério da Saúde/Secretaria de Vigilância em Saúde, Brasil; Processo 475142/2013-6 Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil
Contributed Indexing: Keywords: genotypes; health policies; key populations; resistance-associated substitutions; viral hepatitis
Substance Nomenclature: 0 (Crack Cocaine)
0 (Antiviral Agents)
0 (RNA, Viral)
Entry Date(s): Date Created: 20251231 Date Completed: 20251231 Latest Revision: 20261014
Update Code: 20261014
PubMed Central ID: PMC12735445
DOI: 10.3390/pathogens14121296
PMID: 41471251
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2076-0817
DOI:10.3390/pathogens14121296