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Non-Covalent Spin-Labeling of RNA With Short Hairpins Containing the Rigid Spin Label Çm.

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Τίτλος: Non-Covalent Spin-Labeling of RNA With Short Hairpins Containing the Rigid Spin Label Çm.
Συγγραφείς: Ahmad IM; Department of Chemistry, Science Institute, University of Iceland, Reykjavik, Iceland., Endeward B; Institute of Physical and Theoretical Chemistry, Goethe University, Frankfurt am Main, Germany., Prisner TF; Institute of Physical and Theoretical Chemistry, Goethe University, Frankfurt am Main, Germany., Sigurdsson ST; Department of Chemistry, Science Institute, University of Iceland, Reykjavik, Iceland.
Πηγή: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2026 Feb 16; Vol. 32 (7), pp. e03253. Date of Electronic Publication: 2025 Dec 26.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 9513783 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-3765 (Electronic) Linking ISSN: 09476539 NLM ISO Abbreviation: Chemistry Subsets: MEDLINE
Imprint Name(s): Original Publication: Weinheim, Germany : Wiley-VCH
Ιατρικοί όροι (MeSH): RNA*/chemistry , Spin Labels*, Electron Spin Resonance Spectroscopy/methods ; Oligonucleotides/chemistry ; Nucleic Acid Conformation
Περίληψη: Understanding the structure and dynamics of nucleic acids is essential for elucidating their complex biological functions. Electron Paramagnetic Resonance (EPR) spectroscopy is a valuable technique to extract such information. However, its application to nucleic acids requires the incorporation of spin labels, usually nitroxides. Rigid spin labels are more informative EPR probes than flexible ones, since they offer high-precision distance measurements and can provide information about the relative orientation of spin labels. However, the synthesis and incorporation of such rigid labels is nontrivial. Here we describe a strategy in which the rigid spin label Çm is incorporated into a small hairpin, which in turn can be used to label several different oligonucleotides noncovalently through helical stacking. We have studied the noncovalent assembly of spin-labeled RNA hairpins with RNA duplexes by both continuous wave (CW) and pulsed dipolar EPR spectroscopy, specifically pulsed electron-electron double resonance (PELDOR, also called DEER). Our data shows that short complementary overhangs facilitate efficient helical stacking, opening the possibility of using noncovalent labeling with Çm-modified hairpins in conjunction with pulsed dipolar EPR spectroscopy for structural studies of larger RNAs and RNA-protein complexes.
(© 2025 Wiley‐VCH GmbH.)
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Grant Information: 206708 Icelandic Research Fund; 206708 Icelandic Centre for Research
Contributed Indexing: Keywords: DEER; EPR spectroscopy; PELDOR; nucleic acids; spin labeling
Substance Nomenclature: 0 (Spin Labels)
63231-63-0 (RNA)
0 (Oligonucleotides)
Entry Date(s): Date Created: 20251226 Date Completed: 20260217 Latest Revision: 20260217
Update Code: 20260217
DOI: 10.1002/chem.202503253
PMID: 41452195
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1521-3765
DOI:10.1002/chem.202503253