Academic Journal
Development of enzyme-linked immunosorbent assay with nanogold-labeled probes for detection of diarrhetic shellfish toxins.
| Τίτλος: | Development of enzyme-linked immunosorbent assay with nanogold-labeled probes for detection of diarrhetic shellfish toxins. |
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| Μεταγλωττισμένος Τίτλος: | 腹泻性贝毒纳米金标记探针酶联免疫检测技术. |
| Συγγραφείς: | Zhao R; National Marine Environmental Monitoring Center, Dalian 116023, Liaoning, China., Han L; National Marine Environmental Monitoring Center, Dalian 116023, Liaoning, China., Liu Z; National Marine Environmental Monitoring Center, Dalian 116023, Liaoning, China., Zhang PP; National Marine Environmental Monitoring Center, Dalian 116023, Liaoning, China., Liang YB; National Marine Environmental Monitoring Center, Dalian 116023, Liaoning, China. |
| Πηγή: | Ying yong sheng tai xue bao = The journal of applied ecology [Ying Yong Sheng Tai Xue Bao] 2025 Nov 18; Vol. 36 (11), pp. 3549-3556. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Ying yong sheng tai xue bao bian ji wei yuan hui Country of Publication: China NLM ID: 9425159 Publication Model: Print Cited Medium: Print ISSN: 1001-9332 (Print) Linking ISSN: 10019332 NLM ISO Abbreviation: Ying Yong Sheng Tai Xue Bao Subsets: MEDLINE |
| Imprint Name(s): | Publication: Shenyang Shi : Ying yong sheng tai xue bao bian ji wei yuan hui Original Publication: Shenyang Shi : Ying yong sheng tai xue bao bian ji wei yuan hui, 1990- |
| Ιατρικοί όροι (MeSH): | Marine Toxins*/analysis , Food Contamination*/analysis , Shellfish*/analysis, Gold/chemistry ; Enzyme-Linked Immunosorbent Assay/methods ; Okadaic Acid/analysis ; Okadaic Acid/analogs & derivatives ; Metal Nanoparticles/chemistry ; Pyrans/analysis ; Animals ; Shellfish Poisoning |
| Περίληψη: | Diarrheal shellfish toxins (DSTs) are a type of liposoluble toxins produced by toxic algae in the ocean, with main components of okadaic acid (OA), dinotricin-1 (DTX-1), and dinotricin-2 (DTX-2). They can accumulate in shellfish through food chain. Human consumption of toxic shellfish can have adverse effects on health. Therefore, conducting DSTS detection can effectively ensure food safety. Base on the principle of competitive enzyme-linked immunosorbent assay (ELISA), referring to the seed growth method, we prepared goldnano rods using gold nanoparticles (AuNPs), and conjugated them with okadaic acid antibody-horseradish peroxidase (OA-HRP) to form nanoprobes, which together established a novel ELISA technology of goldnano rods labeled OA-HRP. The technology presented significant detection advantages with a detection limit of 2.32 ng·mL-1, a quantification limit of 2.97 ng·mL-1, and a sample recovery rate of 85.2% to 117.8%. Both the intra-batch and inter-batch varia-tion coefficients were below 20.0%. The proposed method demonstrated high specificity for okadaic acid (OA), dinophysistoxin-1 (DTX-1), and dinophysistoxin-2 (DTX-2), with no cross-reactions observed against six other lipophilic toxins-azaspiracid-1, azaspiracid-2, and azaspiracid-3 (AZA-1, AZA-2, AZA-3), pectenotoxin-2 (PTX-2), yessotoxin (YTX), and homo-yessotoxin (hYTX). Moreover, it showed good correlation with the determination results of liquid chromatography-tandem mass spectrometry (LC-MS/MS). The goldnano rods labeled OA-HRP direct ELISA method established here significantly improved the detection sensitivity of DSTs, which could be used for rapid quantitative detection and analysis of DSTs in seafood, with significant application prospects. |
| Contributed Indexing: | Keywords: diarrhetic shellfish toxin; enzyme-linked immunosorbent assay (ELISA); gold nanoparticle labeling; rapid detection technology Local Abstract: [Publisher, Chinese] 腹泻性贝毒(DSTs)是由海洋中有毒藻产生的一类脂溶性毒素,其主要组分为大田软海绵酸(OA)、鳍藻毒素-1(DTX-1)和鳍藻毒素-2(DTX-2),可通过食物链在贝类体内累积,人类食用有毒贝类会对健康产生不良影响,开展腹泻性贝毒检测可有效保障我国食品安全。本研究依据直接竞争酶联免疫技术(ELISA)原理,参考种子生长法,利用纳米金颗粒(AuNPs)制备纳米金棒,并与大田软海绵酸抗体-辣根过氧化物酶(OA-HRP)偶联合成纳米探针,建立纳米金棒标记-OA-HRP直接ELISA检测技术。该项技术具有明显检测优势,检出限为2.32 ng·mL-1,定量限为2.97 ng·mL-1,样本加标回收率为85.2%～117.8%,批内、批间变异系数均低于20%;该技术对大田软海绵酸(OA)、鳍藻毒素-1(DTX-1)和鳍藻毒素-2(DTX-2)显示出良好的特异性,与其他6种脂溶性毒素——原多甲藻酸(AZA-1、AZA-2、AZA-3)、扇贝毒素-2(PTX-2)、虾夷扇贝毒素(YTX)和类虾夷扇贝毒素(hYTX)无交叉反应,且与液相色谱-串联质谱法(LC-MS/MS)的测定结果具有较好的相关性。本研究建立的纳米金棒标记-OA-HRP直接ELISA技术明显提高了DSTs的检测灵敏度,可用于海产品DSTs的快速定量检测分析,具有重要的应用前景。. |
| Substance Nomenclature: | 0 (Marine Toxins) 7440-57-5 (Gold) 1W21G5Q4N2 (Okadaic Acid) 4Q51CVY9O2 (dinophysistoxin 1) 0 (Pyrans) |
| Entry Date(s): | Date Created: 20251215 Date Completed: 20251215 Latest Revision: 20260127 |
| Update Code: | 20260130 |
| DOI: | 10.13287/j.1001-9332.202511.031 |
| PMID: | 41392687 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1001-9332 |
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| DOI: | 10.13287/j.1001-9332.202511.031 |