Academic Journal
Aptamer-based colorimetric detection of ATP and PDGF-BB by silver nanoparticles-mediated signal amplification and urease activity inhibition.
| Τίτλος: | Aptamer-based colorimetric detection of ATP and PDGF-BB by silver nanoparticles-mediated signal amplification and urease activity inhibition. |
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| Συγγραφείς: | Wang F; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China., Tang Z; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China., Shan X; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China., Li Y; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China., Zhou F; Department of Radiation and Medical Oncology, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China., Ji X; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. xhu@whu.edu.cn., He Z; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. zhkhe@whu.edu.cn.; Department of Radiation and Medical Oncology, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China. zhkhe@whu.edu.cn. |
| Πηγή: | Mikrochimica acta [Mikrochim Acta] 2025 Nov 18; Vol. 192 (12), pp. 825. Date of Electronic Publication: 2025 Nov 18. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Wien ; New York : Springer-Verlag. |
| Ιατρικοί όροι (MeSH): | Colorimetry*/methods , Aptamers, Nucleotide*/chemistry , Silver*/chemistry , Adenosine Triphosphate*/blood , Adenosine Triphosphate*/analysis , Metal Nanoparticles*/chemistry , Urease*/antagonists & inhibitors , Urease*/metabolism , Becaplermin*/blood , Becaplermin*/analysis , Biosensing Techniques*/methods, Humans ; Limit of Detection |
| Περίληψη: | A novel colorimetric aptasensor based on silver nanoparticles (AgNPs) and Ag+-dependent urease inhibition has been designed. Sensitive detection of adenosine triphosphate (ATP) and platelet-derived growth factor BB (PDGF-BB) were achieved by employing the competitive assay and sandwich assay, respectively. The method conjugated the aptamer with magnetic beads to construct a magnetic recognition module, and employed poly-C DNA modified AgNPs as the signal amplification module. Target binding to the magnetic recognition module triggers magnetic capture of AgNPs, which were subsequently oxidized to release abundant Ag+. These ions efficiently inhibited urease activity, thus blocking urea hydrolysis and preventing pH increase, thereby achieving cascaded signal amplification. The colorimetric mechanism of this method relies on converting the resultant pH change into a color transition of the acid-base indicator phenol red, with the absorbance ratio of A (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Grant Information: | 22174101 National Natural Science Foundation of China; 22174102 National Natural Science Foundation of China |
| Contributed Indexing: | Keywords: Aptamer; Colorimetric assay; Silver nanoparticles; Urease catalysis |
| Substance Nomenclature: | 0 (Aptamers, Nucleotide) 3M4G523W1G (Silver) 8L70Q75FXE (Adenosine Triphosphate) EC 3.5.1.5 (Urease) 1B56C968OA (Becaplermin) |
| Entry Date(s): | Date Created: 20251118 Date Completed: 20251118 Latest Revision: 20261010 |
| Update Code: | 20261010 |
| DOI: | 10.1007/s00604-025-07686-w |
| PMID: | 41251980 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1436-5073 |
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| DOI: | 10.1007/s00604-025-07686-w |